OPTINEURIN | Ubiquitylation Resource

Plasmid

pCMV-FLAG Optineurin M98K

Parent Plasmid

pCMV5

Expressed

FLAG-Optineurin M98K

DU Number

DU8909

Genbank

NP_001008212

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAG
CCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACC
TGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTG
ACCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCAT
GAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAG
AACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAAAG
GCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAA
AGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTC
CCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTG
AGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCA
GCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTA
GGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGT
CCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAATATAG
GAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAA
CTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAG
AGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGA
AAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCA
CAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAA
GTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGA
GGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAG
AAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTG
AATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGT
GGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATG
AAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTCTTCAA
GAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTC
AGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAAC
TGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAG
CAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAG
GGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAGAGCAGCGA
GAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTT
CTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGAT
GGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGG
CTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGG
AATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACAT
AGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEE
LLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQ
SKEAKERLKALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQE
KDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAE
GSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLC
LREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEE
KGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALER
KNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVL
QMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALAS
KQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEK
EQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGA
EDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 67187 Daltons

100042 R 1000 1001 1047 19007