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A20 (mouse)

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Plasmid
pGEX6P-1-A20 C103S (mouse)
Parent Plasmid
pGEX6P1
Expressed
GST-A20 C103S (mouse)
DU Number
DU8469
Genbank
AAH60221
Tag
GST
Species
Mouse
Sequence of Insert
GGATCCATGGCTGAACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATAT
GCGGAAAGCTGTGAAGATACGAGAGAGAACCCCAGAAGACATTTTCAAAC
CTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACACGCTG
GAGATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCA
CAAAGCACTTATTGACAGAAGTGTCCAGGCTTCCCTGGAAAGCCAGAAGA
AGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGGCTCTGAAAACCAAT
GGTGATGGAAACTCCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGT
TCAGGATACTGACCTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGG
AGACAGACACTCGGAACTTTAAATTCCGCTGGCAGCTGGAATCTCTGAAA
TCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACTGGAA
TGACGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAG
CCCGAAGTGGACTTCAGTACAATTCCCTGGAAGAAATCCACATATTTGTC
CTCAGCAACATCCTCAGAAGACCCATCATTGTCATTTCAGACAAAATGCT
AAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGA
TTTATCTGCCTCTTCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATC
GTCCTAGGCTATGACAGCCAGCACTTTGTACCCCTGGTGACCCTGAAGGA
CAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGGGGTA
GGTTTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATG
AAGGAAAAGCTTCTAAAGGAGTACTTGATAGTGATGGAGATCCCTGTGCA
AGGCTGGGACCACGGCACGACTCACCTGATCAACGCTGCAAAATTGGATG
AAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTT
GTTCAGCACGAATACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAG
AGCGGCACATGCGCAGAACCCCTTGGAGCCTTCCACACCCCAGCTATCAC
TCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTCCGTG
AACACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCA
GAGCAAGCTCCCAAAGCTGAACTCGAAGCTAGGCCCTGAAGGACTCCCAG
GCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGGAAACCGCTGGAGGA
CCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGAC
CACTGCAATGAAGTGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGC
AGCATAATGGATTCTGTGAGCGTTGCCACGCCCGGCAGATTAATGCCAGC
CACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCACTCG
GACCTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGC
CCAGCTCCAGCCTCACTTCCAGTATCCCTGCCTCCTGTCACCAACGCTCC
AAGTCTGACCCCTCACAACTCATCCAAAGTCTCACTCCACACTCTTGCCA
CCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGA
CTCCAGGAGACAGAGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATG
TATTTTGGGACTCCAGAAAACAAGGGCTTTTGCACTCTATGTTTCATCGA
ATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGTTCCC
CGGCCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGC
ACACTCGGAAGCACCATGTTTGAAGGGTACTGTCAGAAGTGTTTCATCGA
AGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAACGGAAGAACAGCTGA
GATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGG
CTGAAATGTGCCCGGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGA
GGAACTCTGTATGGAGTGCCAGCACCTAAGCCAACGAGTAGGTTCTGTGG
CCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGG
GCCCCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAA
TGAGTGCTACCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEQLLPQALYLSNMRKAV
KIRERTPEDIFKPTNGIIYHFKTMHRYTLEMFRTCQFCPQFREIIHKALI
DRSVQASLESQKKLNWCREVRKLVALKTNGDGNSLMHAACQYMWGVQDTD
LVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRNWNDEWD
NLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSLE
SGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPE
LRAVPLVNRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDH
GTTHLINAAKLDEANLPKEINLVDDYFELVQHEYKKWQENSDQARRAAHA
QNPLEPSTPQLSLMDIKCETPNCPFFMSVNTQPLFHECSERRQKNQSKLP
KLNSKLGPEGLPGVGLGSSNWSPEETAGGPHSAPPTAPSLFLFSETTAMK
CRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQACLQDVTRTFNG
ICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHRTGN
VSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYREN
KQSVTASEKAGSPAPRFQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQ
RFHEARRTEEQLRSSQHRDMPRTTQVASRLKCARASCKNILACRSEELCM
ECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGYCNECYQ
FKQMYG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for bacterial expression.

Estimated MW of expressed protein : 114502 Daltons
100042 R 1000 1001 1047 19007
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