USP7 | Ubiquitylation Resource

Plasmid

pCMV-FLAG-1 USP7 50-550

Parent Plasmid

pCMV5 FLAG1

Expressed

FLAG USP7 50-550

DU Number

DU32699

Genbank

NP_003461.2

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACACCAG
TTGGCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGAC
TGAGTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGCGAAATCTGCCATGG
AAGATTATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAG
CGTAGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGT
CTTGCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAA
AAGTCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGA
TTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGA
AAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCG
GATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGT
CGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGA
CGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACC
GAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTT
CTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAA
AGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAG
GAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCAC
CTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCT
ATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTAT
TATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATT
TGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACG
CTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACA
TTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCA
GACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGT
TACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAAT
TATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACA
TTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTG
ATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAAT
TATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTA
CATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGG
TCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAG
AAAAGGATCTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSP
PCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVL
KIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDK
VTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQL
RKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETL
DSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVD
YRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQE
AEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQ
KTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRC
TKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQ
QLVERLQEEKRI*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression

Estimated MW of expressed protein : 59367 Daltons

100042 R 1000 1001 1047 19007