BIRC3 | Ubiquitylation Resource

Plasmid

pCMV-FLAG-1 BIRC3

Parent Plasmid

pCMV5 FLAG1

Expressed

FLAG BIRC3

DU Number

DU32470

Genbank

NP_001156.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGAACATAGTAGAAAACAGCATATTCTTATCAAATTTGATGAAA
AGCGCCAACACGTTTGAACTGAAATACGACTTGTCATGTGAACTGTACCG
AATGTCTACGTATTCCACTTTTCCTGCTGGGGTTCCTGTCTCAGAAAGGA
GTCTTGCTCGTGCTGGTTTCTATTACACTGGTGTGAATGACAAGGTCAAA
TGCTTCTGTTGTGGCCTGATGCTGGATAACTGGAAAAGAGGAGACAGTCC
TACTGAAAAGCATAAAAAGTTGTATCCTAGCTGCAGATTCGTTCAGAGTC
TAAATTCCGTTAACAACTTGGAAGCTACCTCTCAGCCTACTTTTCCTTCT
TCAGTAACAAATTCCACACACTCATTACTTCCGGGTACAGAAAACAGTGG
ATATTTCCGTGGCTCTTATTCAAACTCTCCATCAAATCCTGTAAACTCCA
GAGCAAATCAAGATTTTTCTGCCTTGATGAGAAGTTCCTACCACTGTGCA
ATGAATAACGAAAATGCCAGATTACTTACTTTTCAGACATGGCCATTGAC
TTTTCTGTCGCCAACAGATCTGGCAAAAGCAGGCTTTTACTACATAGGAC
CTGGAGACAGAGTGGCTTGCTTTGCCTGTGGTGGAAAATTGAGCAATTGG
GAACCGAAGGATAATGCTATGTCAGAACACCTGAGACATTTTCCCAAATG
CCCATTTATAGAAAATCAGCTTCAAGACACTTCAAGATACACAGTTTCTA
ATCTGAGCATGCAGACACATGCAGCCCGCTTTAAAACATTCTTTAACTGG
CCCTCTAGTGTTCTAGTTAATCCTGAGCAGCTTGCAAGTGCGGGTTTTTA
TTATGTGGGTAACAGTGATGATGTCAAATGCTTTTGCTGTGATGGTGGAC
TCAGGTGTTGGGAATCTGGAGATGATCCATGGGTTCAACATGCCAAGTGG
TTTCCAAGGTGTGAGTACTTGATAAGAATTAAAGGACAGGAGTTCATCCG
TCAAGTTCAAGCCAGTTACCCTCATCTACTTGAACAGCTGCTATCCACAT
CAGACAGCCCAGGAGATGAAAATGCAGAGTCATCAATTATCCATTTTGAA
CCTGGAGAAGACCATTCAGAAGATGCAATCATGATGAATACTCCTGTGAT
TAATGCTGCCGTGGAAATGGGCTTTAGTAGAAGCCTGGTAAAACAGACAG
TTCAGAGAAAAATCCTAGCAACTGGAGAGAATTATAGACTAGTCAATGAT
CTTGTGTTAGACTTACTCAATGCAGAAGATGAAATAAGGGAAGAGGAGAG
AGAAAGAGCAACTGAGGAAAAAGAATCAAATGATTTATTATTAATCCGGA
AGAATAGAATGGCACTTTTTCAACATTTGACTTGTGTAATTCCAATCCTG
GATAGTCTACTAACTGCCGGAATTATTAATGAACAAGAACATGATGTTAT
TAAACAGAAGACACAGACGTCTTTACAAGCAAGAGAACTGATTGATACGA
TTTTAGTAAAAGGAAATATTGCAGCCACTGTATTCAGAAACTCTCTGCAA
GAAGCTGAAGCTGTGTTATATGAGCATTTATTTGTGCAACAGGACATAAA
ATATATTCCCACAGAAGATGTTTCAGATCTACCAGTGGAAGAACAATTGC
GGAGACTACAAGAAGAAAGAACATGTAAAGTGTGTATGGACAAAGAAGTG
TCCATAGTGTTTATTCCTTGTGGTCATCTAGTAGTATGCAAAGATTGTGC
TCCTTCTTTAAGAAAGTGTCCTATTTGTAGGAGTACAATCAAGGGTACAG
TTCGTACATTTCTTTCATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTF
PAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKL
YPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYS
NSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDL
AKAGFYYIGPGDRVACFACGGKLSNWEPKDNAMSEHLRHFPKCPFIENQL
QDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDD
VKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYP
HLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMG
FSRSLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEK
ESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTS
LQARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDV
SDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCP
ICRSTIKGTVRTFLS*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression

Estimated MW of expressed protein : 69639 Daltons

100042 R 1000 1001 1047 19007