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A20

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Plasmid
pCMV-HA-2 A20
Parent Plasmid
pCMV5 HA2
Expressed
HA A20
DU Number
DU32903
Genbank
BC114480
Tag
HA
Species
Human
Sequence of Insert
GCGGCCGCGATGGCTGAACAAGTCCTTCCTCAGGCTTTGTATTTGAGCAA
TATGCGGAAAGCTGTGAAGATACGGGAGAGAACTCCAGAAGACATTTTTA
AACCTACTAATGGGATCATTCATCATTTTAAAACCATGCACCGATACACA
CTGGAAATGTTCAGAACTTGCCAGTTTTGTCCTCAGTTTCGGGAGATCAT
CCACAAAGCCCTCATCGACAGAAACATCCAGGCCACCCTGGAAAGCCAGA
AGAAACTCAACTGGTGTCGAGAAGTCCGGAAGCTTGTGGCGCTGAAAACG
AACGGTGACGGCAATTGCCTCATGCATGCCACTTCTCAGTACATGTGGGG
CGTTCAGGACACAGACTTGGTACTGAGGAAGGCGCTGTTCAGCACGCTCA
AGGAAACAGACACACGCAACTTTAAATTCCGCTGGCAACTGGAGTCTCTC
AAATCTCAGGAATTTGTTGAAACGGGGCTTTGCTATGATACTCGGAACTG
GAATGATGAATGGGACAATCTTATCAAAATGGCTTCCACAGACACACCCA
TGGCCCGAAGTGGACTTCAGTACAACTCACTGGAAGAAATACACATATTT
GTCCTTTGCAACATCCTCAGAAGGCCAATCATTGTCATTTCAGACAAAAT
GCTAAGAAGTTTGGAATCAGGTTCCAATTTCGCCCCTTTGAAAGTGGGTG
GAATTTACTTGCCTCTCCACTGGCCTGCCCAGGAATGCTACAGATACCCC
ATTGTTCTCGGCTATGACAGCCATCATTTTGTACCCTTGGTGACCCTGAA
GGACAGTGGGCCTGAAATCCGAGCTGTTCCACTTGTTAACAGAGACCGGG
GAAGATTTGAAGACTTAAAAGTTCACTTTTTGACAGATCCTGAAAATGAG
ATGAAGGAGAAGCTCTTAAAAGAGTACTTAATGGTGATAGAAATCCCCGT
CCAAGGCTGGGACCATGGCACAACTCATCTCATCAATGCCGCAAAGTTGG
ATGAAGCTAACTTACCAAAAGAAATCAATCTGGTAGATGATTACTTTGAA
CTTGTTCAGCATGAGTACAAGAAATGGCAGGAAAACAGCGAGCAGGGGAG
GAGAGAGGGGCACGCCCAGAATCCCATGGAACCTTCCGTGCCCCAGCTTT
CTCTCATGGATGTAAAATGTGAAACGCCCAACTGCCCCTTCTTCATGTCT
GTGAACACCCAGCCTTTATGCCATGAGTGCTCAGAGAGGCGGCAAAAGAA
TCAAAACAAACTCCCAAAGCTGAACTCCAAGCCGGGCCCTGAGGGGCTCC
CTGGCATGGCGCTCGGGGCCTCTCGGGGAGAAGCCTATGAGCCCTTGGCG
TGGAACCCTGAGGAGTCCACTGGGGGGCCTCATTCGGCCCCACCGACAGC
ACCCAGCCCTTTTCTGTTCAGTGAGACCACTGCCATGAAGTGCAGGAGCC
CCGGCTGCCCCTTCACACTGAATGTGCAGCACAACGGATTTTGTGAACGT
TGCCACAACGCCCGGCAACTTCACGCCAGCCACGCCCCAGACCACACAAG
GCACTTGGATCCCGGGAAGTGCCAAGCCTGCCTCCAGGATGTTACCAGGA
CATTTAATGGGATCTGCAGTACTTGCTTCAAAAGGACTACAGCAGAGGCC
TCCTCCAGCCTCAGCACCAGCCTCCCTCCTTCCTGTCACCAGCGTTCCAA
GTCAGATCCCTCGCGGCTCGTCCGGAGCCCCTCCCCGCATTCTTGCCACA
GAGCTGGAAACGACGCCCCTGCTGGCTGCCTGTCTCAAGCTGCACGGACT
CCTGGGGACAGGACGGGGACGAGCAAGTGCAGAAAAGCCGGCTGCGTGTA
TTTTGGGACTCCAGAAAACAAGGGCTTTTGCACACTGTGTTTCATCGAGT
ACAGAGAAAACAAACATTTTGCTGCTGCCTCAGGGAAAGTCAGTCCCACA
GCGTCCAGGTTCCAGAACACCATTCCGTGCCTGGGGAGGGAATGCGGCAC
CCTTGGAAGCACCATGTTTGAAGGATACTGCCAGAAGTGTTTCATTGAAG
CTCAGAATCAGAGATTTCATGAGGCCAAAAGGACAGAAGAGCAACTGAGA
TCGAGCCAGCGCAGAGATGTGCCTCGAACCACACAAAGCACCTCAAGGCC
CAAGTGCGCCCGGGCCTCCTGCAAGAACATCCTGGCCTGCCGCAGCGAGG
AGCTCTGCATGGAGTGTCAGCATCCCAACCAGAGGATGGGCCCTGGGGCC
CACCGGGGTGAGCCTGCCCCCGAAGACCCCCCCAAGCAGCGTTGCCGGGC
CCCCGCCTGTGATCATTTTGGCAATGCCAAGTGCAACGGCTACTGCAACG
AATGCTTTCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MYPYDVPDYAGIPGIPGSTRAAAMAEQVLPQALYLSNMRKAVKIRERTPE
DIFKPTNGIIHHFKTMHRYTLEMFRTCQFCPQFREIIHKALIDRNIQATL
ESQKKLNWCREVRKLVALKTNGDGNCLMHATSQYMWGVQDTDLVLRKALF
STLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRNWNDEWDNLIKMAST
DTPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSLESGSNFAPL
KVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLVN
RDRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINA
AKLDEANLPKEINLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSV
PQLSLMDVKCETPNCPFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGP
EGLPGMALGASRGEAYEPLAWNPEESTGGPHSAPPTAPSPFLFSETTAMK
CRSPGCPFTLNVQHNGFCERCHNARQLHASHAPDHTRHLDPGKCQACLQD
VTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQRSKSDPSRLVRSPSPH
SCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFCTLC
FIEYRENKHFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKC
FIEAQNQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILAC
RSEELCMECQHPNQRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNG
YCNECFQFKQMYG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter HA for mammalian expression

Estimated MW of expressed protein : 91975 Daltons
100042 R 1000 1001 1047 19007
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