optineurin | Ubiquitylation Resource

Plasmid

pCMV5 Optineurin HA

Parent Plasmid

pCMV5

Expressed

Optineurin HA

DU Number

DU12345

Genbank

NP_001008212

Tag

HA

Species

Human

Sequence of Insert

View Sequence

GAATTCGCCACCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGA
GGACAGCCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACC
CAAACCTGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAG
CTCCTGACCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCA
AGCCATGAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGA
AGGAAGAACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGT
CTAATGGCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAA
ACTAAAAGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCA
GGCTTCCCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAG
GTGGTGAGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGA
ACTGCAGCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTG
AAATTAGGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAG
CATAGTCCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAA
ATATAGGAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGG
AGTTAACTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAG
GTGGAGAGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGA
TTTTGAAAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGG
GGAGCACAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGA
AGCGAAGTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCT
TCAAGAGGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGAC
TTCAAGAAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCA
GAGTTGAATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCT
ACAAGTGGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAG
AGGATGAAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTT
CTTCAAGAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAA
AGAGTCAGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAAC
TGGAACTGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAA
ATGAAGCAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCAT
CCTCAGGGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAGAG
CAGCGAGAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTG
GCAGTTCTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTC
CTTGATGGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACC
AGCAGGCTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAA
CAGCGGAATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCC
TGACATAGACACGTTACAGATTCACGTGATGGATTGCATCATTATGTACC
CATACGATGTGCCAGATTACGCCTAAGGATCC

Amino Acid Sequence

View Sequence

MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTE
NHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMAL
SHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRL
QAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPG
PTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERL
EVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVE
ALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNE
KQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEH
NNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQT
IAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLL
KENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNI
PIHSCPKCGEVLPDIDTLQIHVMDCIIMYPYDVPDYA*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter HA for mammalian expression.

Estimated MW of expressed protein : 67135 Daltons

100042 R 1000 1001 1047 19007