HOIP | Ubiquitylation Resource

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Plasmid

pGEX6P-2-HOIP

Parent Plasmid

pGEX6P2

Expressed

GST HOIP

DU Number

DU15972

Genbank

NM_017999.4

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCGATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCG
CGAGGAGCTGGCGAGCGCCCTGAGGAGGGATTCCGGGCAGGCGTTTTCCC
TGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCGC
TACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGA
GCCCCGAAACTACCTCAACACCCTGTCCACGGCTCTGAACATCCTGGAGA
AATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGTACTGGCGTGGT
GTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGG
GGGCCGAGATGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATG
GGTTGAGCTTCCCCGAAGGGCAGGAGGAGCCAGATGAGCACCAGGTTGCT
ACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTATT
GCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACA
AGGTTGAAGATGATATGCTGCAGCTTTCAGAATTTGACCCCCTATTGAGA
GAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCAGGCTCCACTCC
TGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCAT
CCTGTAAACAGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACAC
CCATCCCGTGCTCATCACCTCCGCCAGACCCTGCCTGGGGTCCTGCAGGG
TACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAGT
CGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCT
GCAAGTGCTCATTTGCCCTGGCACTGTGCTGCCTGTGCCATGCTAAATGA
GCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCGAGGCTGTAAGG
GGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCCTAGAACCT
GATCTTGCACGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGA
GGCAGCTGCTGTGCTATGTTCCATATGTGAGCGACCTCGGCTGGCCCAGC
CTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACCC
CTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTA
CTGTATTCACTGTACCTTCTGCAACTCGAGCCCTGGCTGGGTGTGTGTTA
TGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATGCCCCCCGGCCC
TATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACG
CCTTAGTGCCCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCC
AAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAA
GGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTA
CTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACG
TCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGT
GCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAA
CCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGC
AGGAGCTCCAGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCA
TTGTTCCAGCACGGAGGTGATGTGTCACGGGCCCTGACTGAGCTACAGCG
CCAACGCCTAGAGCCCTTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGC
CCACCCCTTCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTT
TTGGCAGTCTACGCACTCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTC
ACTGCTGCAGGAGACACCCAGGAACTATGAGTTGGGGGATGTGGTAGAAG
CTGTGAGGCACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAG
GAGTGTGCCGTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCT
GACTTCCTGTGAGTGCACCATCTGTCCTGACTGCTTCCGCCAGCACTTCA
CCATCGCCTTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGT
GGCCGCCCCGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTAC
CCTTGACATCCAGCTTCGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGT
TCCATAAGAAGCTGACCGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTG
TGGTGTGCCCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGA
GGCAACTTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCC
AGTGGGAGGAGCAGCACCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGG
AAACGCATGAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATCT
TCAGGAAAACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGG
CCCGAGGAGGCTGCATGCACTTTCACTGTACCCAGTGCCGCCACCAGTTC
TGCAGCGGCTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGAGCC
TAACTGCAGGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAGACTGCC
TCTTCTACCTGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAG
GACAATAACGTCATGTTTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGT
CCCTGGAGGCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGC
TCAGGGACGAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTG
TGCCAGGCACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACTC
GCTGGACCCAGCCACCTTGTATGAGGTGGAAGAGCTGGAGACGGCCACTG
AGCGCTACCTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCT
GCTTACCAGGCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGG
ACAGAGTATCCCCCGCAGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMPGEEEER
AFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVR
CNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRST
VDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRT
ELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPS
VPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTL
PGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAA
CAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQS
CTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASA
QSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPK
PGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEI
FSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAW
LDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRA
LTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGR
AELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPH
NRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQL
LSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIY
EREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQ
GLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAK
NKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPP
AGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVS
LINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLT
EEVPLGQSIPRRRK*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 147449 Daltons

100042 R 1000 1001 1047 19007

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