HOIL | Ubiquitylation Resource

Synonyms

XAP3; XAP4; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; HOIL-1; ZRANB4; C20orf18; UBCE7IP3; RBCK1

Plasmid

pET15b 6His HOIL

Parent Plasmid

pET15b 6His 3C

Expressed

6His 3C HOIL

DU Number

DU32155

Genbank

NM_031229.4 NP_112506.2

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCGATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAG
CCTCACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTG
CCATCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCT
GAGGTCTCCCCAACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGC
TCAGATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGACAG
TGGCGTCTCTCAAGGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTC
TTGCAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCAGGAGACCCT
GCACTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGC
TGTCAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGG
CAGCTGCGGATGCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCC
GCGGGGCCCTCTGGAGCCAGGCCCCCCAAAGCCCGGGGTCCCCCAGGAAC
CCGGACGGGGGCAGCCAGATGCAGTGCCTGAGCCCCCACCGGTGGGCTGG
CAGTGCCCCGGGTGCACCTTCATCAACAAGCCCACGCGGCCTGGCTGTGA
GATGTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACC
AGCCCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTG
CGTCAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCA
GCACGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCG
AGTGCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTG
CGTGAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCG
CAACAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACT
CGTGCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCT
GAGGATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCG
CAGTGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCT
TCTTTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTC
AACTGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTA
TCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGA
CGACAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGC
CCCCAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCG
CTGCACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCCCACGCT
GGGGCCCTGGGGGCCCAGGAGACACCAGCGGGGGCTGCCGCTGCAGGGTA
AATGGGATTCCTTGCCACCCAAGCTGTCAGAACTGCCACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMDEKTKKAEEMALSL
TRAVAGGDEQVAMKCAIWLAEQRVPLSVQLKPEVSPTQDIRLWVSVEDAQ
MHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLARDQETLH
SHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFKDLTLQPR
GPLEPGPPKPGVPQEPGRGQPDAVPEPPPVGWQCPGCTFINKPTRPGCEM
CCRARPEAYQVPASYQPDEEERARLAGEEEALRQYQQRKQQQQEGNYLQH
VQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRN
SQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRS
AFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQ
EDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGCDWIRC
TVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter 6HIS for bacterial expression

Estimated MW of expressed : 61184 Daltons

100042 R 1000 1001 1047 19007