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MEKK1

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Synonyms
MAP3K1
Plasmid
pGFP TAPP new MEKK1 D1-622 human
Parent Plasmid
pGFP TAP BN
Expressed
GFP-TAP-new MEKK1 D1-622 human
DU Number
DU3256
Genbank
AF042838
Tag
GFP
TAP
Species
Human
Sequence of Insert
GGATCCATGGTTACTACAGTACCCCATGTGTTTTCAAAACTGTTAGAAAT
GCTGAGTGTTTCCAGTTCCACTCACTTCACCAGGATGCGTCGCCGTTTGA
TGGCTATTGCAGATGAGGTGGAAATTGCCGAAGCCATCCAGTTGGGCGTA
GAAGACACTTTGGATGGTCAACAGGACAGCTTCTTGCAGGCATCTGTTCC
CAACAACTATCTGGAAACCACAGAGAACAGTTCCCCTGAGTGCACAATCC
ATTTAGAGAAAACTGGAAAAGGATTATGTGCTACAAAATTGAGTGCCAGT
TCAGAGGACATTTCTGAGAGACTGGCCAGCATTTCAGTAGGACCTTCTAG
TTCAACAACAACAACAACAACAACAGAGCAACCAAAGCCAATGGTTCAAA
CAAAAGGCAGACCCCACAGTCAGTGTTTGAACTCCTCTCCTTTATCTCAT
CATTCCCAATTAATGTTTCCAGCCTTGTCAACCCCTTCTTCTTCTACCCC
ATCTGTACCAGCTGGCACTGCAACAGATGTCTCTAAGCATAGACTTCAGG
GATTCATTCCCTGCAGAATACCTTCTGCATCTCCTCAAACACAGCGCAAG
TTTTCTCTACAATTCCACAGAAACTGTCCTGAAAACAAAGACTCAGATAA
ACTTTCCCCAGTCTTTACTCAGTCAAGACCCTTGCCCTCCAGTAACATAC
ACAGGCCAAAGCCATCTCGACCTACCCCAGGTAATACAAGTAAACAGGGA
GATCCCTCAAAAAATAGCATGACACTTGATCTGAACAGTAGTTCCAAATG
TGATGACAGCTTTGGCTGTAGCAGCAATAGTAGTAATGCTGTTATACCCA
GTGACGAGACAGTGTTCACCCCAGTAGAGGAGAAATGCAGATTAGATGTC
AATACAGAGCTCAACTCCAGTATTGAGGACCTTCTTGAAGCATCTATGCC
TTCAAGTGATACAACAGTAACTTTTAAGTCAGAAGTTGCTGTCCTGTCTC
CTGAAAAGGCTGAAAATGATGATACCTACAAAGATGATGTGAATCATAAT
CAAAAGTGCAAAGAGAAGATGGAAGCTGAAGAAGAAGAAGCTTTAGCAAT
TGCCATGGCAATGTCAGCGTCTCAGGATGCCCTCCCCATAGTTCCTCAGC
TGCAGGTTGAAAATGGAGAAGATATCATCATTATTCAACAGGATACACCA
GAGACTCTACCAGGACATACCAAAGCAAAACAACCGTATAGAGAAGACAC
TGAATGGCTGAAAGGTCAACAGATAGGCCTTGGAGCATTTTCTTCTTGTT
ATCAGGCTCAAGATGTGGGAACTGGAACTTTAATGGCTGTTAAACAGGTG
ACTTATGTCAGAAACACATCTTCTGAGCAAGAAGAAGTAGTAGAAGCACT
AAGAGAAGAGATAAGAATGATGAGCCATCTGAATCATCCAAACATCATTA
GGATGTTGGGAGCCACGTGTGAGAAGAGCAATTACAATCTCTTCATTGAA
TGGATGGCAGGGGGATCGGTGGCTCATTTGCTGAGTAAATATGGAGCCTT
CAAAGAATCAGTAGTTATTAACTACACTGAACAGTTACTCCGTGGCCTTT
CGTATCTCCATGAAAACCAAATCATTCACAGAGATGTCAAAGGTGCCAAT
TTGCTAATTGACAGCACTGGTCAGAGACTAAGAATTGCAGATTTTGGAGC
TGCAGCCAGGTTGGCATCAAAAGGAACTGGTGCAGGAGAGTTTCAGGGAC
AATTACTGGGGACAATTGCATTTATGGCACCTGAGGTACTAAGAGGTCAA
CAGTATGGAAGGAGCTGTGATGTATGGAGTGTTGGCTGTGCTATTATAGA
AATGGCTTGTGCAAAACCACCATGGAATGCAGAAAAACACTCCAATCATC
TTGCTTTGATATTTAAGATTGCTAGTGCAACTACTGCTCCATCGATCCCT
TCACATTTGTCTCCTGGTTTACGAGATGTGGCTCTTCGTTGTTTAGAACT
TCAACCTCAGGACAGACCTCCATCAAGAGAGCTACTGAAGCATCCAGTCT
TTCGTACTACATGGCATCATCACCATCACCATTGAGAATTCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMVT
TVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLD
GQQDSFLQASVPNNYLETTENSSPECTIHLEKTGKGLCATKLSASSEDIS
ERLASISVGPSSSTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLM
FPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQF
HRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKN
SMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELN
SSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKE
KMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPG
HTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRN
TSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGG
SVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDS
TGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRS
CDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSP
GLRDVALRCLELQPQDRPPSRELLKHPVFRTTWHHHHHH*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal GFP TAPP/ C terminal 6His for mammalian expression n.b. numbering in name relates to Celera protein hCP1814173. M1 of hCP1814173 is M145 in AF042838
100042 R 1000 1001 1047 19007
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