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Synonyms
PIG53, NEDD4-1, RPF1
Plasmid
pcDNA5 FRT/TO GFP NEDD4
Parent Plasmid
pcDNA5 FRT/TO GFP
Expressed
GFP-NEDD4
DU Number
DU19742
Genbank
NM_006154.2
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA GTTGATTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMATCAV
EVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVL
TSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQV
DVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSG
SEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGR
TYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETES
VDNQESSENWEIIREDEATMYSSQAFPSPPPSSNLDVPTHLAEELNARLT
IFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEE
KQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTS
DSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKI
PAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRL
ENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDS
YRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYG
LFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFF
IRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEEL
FGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGF
FELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQ
WFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWG
TPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMATCAV
EVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVL
TSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQV
DVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSG
SEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGR
TYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETES
VDNQESSENWEIIREDEATMYSSQAFPSPPPSSNLDVPTHLAEELNARLT
IFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEE
KQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTS
DSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKI
PAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRL
ENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDS
YRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYG
LFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFF
IRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEEL
FGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGF
FELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQ
WFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWG
TPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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