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Plasmid
pET43 DU FAN1 373-end
Parent Plasmid
pET43D 6His
Expressed
NUS-6xHis-S.TAG-Prec-FAN1 373-end
DU Number
DU37212
Genbank
NM_014967.4
Tag
6His
Species
Human
Sequence of Insert
View Sequence
AGATCTATGCCTTACTACCTTCGGAGTTTCCTTGTGGTGCTGAAAACCGTACTTGAGAATGAAGATGATATGTTGCTCTTTGATGAGCAGGAGAAGGGAATTGTAACTAAATTTTATCAGTTATCAGCTACTGGTCAGAAGTTATATGTAAGGCTCTTTCAACGTAAATTAAGCTGGATTAAGATGACCAAATTAGAGTATGAAGAGATTGCCTTAGACTTAACACCTGTGATTGAAGAATTGACGAATGCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGAAGTGCTTGAACTCCTTTCTGCTCCTGAACTAAAATCCCTAGCCAAGACCTTCCACTTGGTGAATCCCAATGGACAGAAACAGCAGCTGGTGGACGCCTTTCTCAAATTGGCCAAACAGCGTTCAGTCTGCACTTGGGGCAAGAATAAGCCTGGAATTGGTGCAGTGATTTTAAAAAGAGCCAAAGCCTTGGCTGGACAGTCAGTACGAATCTGTAAAGGCCCCAGGGCTGTGTTTTCCCGCATCTTGCTACTGTTTTCGTTGACCGACTCAATGGAAGATGAAGACGCCGCTTGTGGAGGTCAGGGACAGCTTTCAACAGTCCTGTTGGTCAACCTCGGCCGAATGGAGTTTCCTAGTTACACCATCAATCGGAAAACCCACATCTTCCAAGACAGAGATGATCTTATCAGATATGCAGCAGCCACGCACATGCTGAGTGACATTTCTTCCGCAATGGCCAATGGGAACTGGGAAGAAGCTAAGGAGCTCGCTCAGTGTGCAAAAAGGGATTGGAACAGACTGAAAAACCACCCTTCTCTGAGATGCCACGAAGATTTACCACTCTTCCTGCGGTGTTTCACTGTTGGGTGGATTTATACAAGGATTTTGTCTCGGTTTGTGGAAATACTGCAGAGACTTCACATGTATGAGGAAGCCGTCAGAGAACTTGAAAGCCTTTTGTCTCAGAGAATTTATTGTCCTGACAGCAGAGGCCGATGGTGGGATCGACTGGCCCTTAATTTACACCAGCACTTGAAGCGCCTGGAACCGACTATCAAGTGCATCACAGAGGGGCTGGCGGATCCGGAAGTCAGAACGGGACACCGCCTTTCACTGTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGTAAAAAGTTCAAGCACCTCTTCCAGCAGCTCCCAGAAATGGCTGTGCAAGATGTGAAACACGTGACCATCACAGGCAGGCTGTGCCCACAGCGTGGGATGTGCAAGTCTGTGTTTGTGATGGAGGCCGGGGAGGCCGCTGACCCCACCACGGTCCTGTGCTCTGTGGAGGAGCTGGCACTGGCCCATTACAGACGCAGCGGTTTTGACCAGGGGATTCATGGCGAAGGGTCCACCTTCAGCACCCTGTATGGCCTCCTCCTGTGGGACATCATCTTCATGGATGGGATTCCGGATGTCTTCAGAAACGCCTGTCAGGCATTCCCCCTGGACTTGTGCACAGACAGCTTCTTCACAAGCAGACGCCCAGCCCTTGAGGCCAGGCTGCAGCTGATTCATGATGCCCCCGAGGAGAGCCTGCGGGCCTGGGTGGCAGCCACGTGGCATGAGCAGGAAGGCAGAGTGGCTTCCCTTGTCAGCTGGGATCGCTTCACGTCTCTTCAGCAAGCTCAGGATCTTGTCTCCTGCCTGGGGGGCCCTGTGCTCAGTGGTGTGTGCAGGCACCTGGCTGCTGACTTTCGACACTGTCGAGGGGGCCTCCCCGACCTGGTGGTGTGGAACTCCCAGAGCCGTCACTTTAAGCTGGTGGAAGTTAAAGGCCCCAATGATCGTCTTTCACATAAGCAGATGATCTGGCTGGCTGAACTGCAGAAGCTGGGGGCTGAAGTAGAAGTCTGCCACGTGGTTGCAGTTGGAGCTAAGAGCCAAAGCCTTAGCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARLEVLFQGPGSMPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTESELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAKQRSVCTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLTDSMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRYAAATHMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPLFLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRGRWWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRESPSCKKFKHLFQQLPEMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAADPTTVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGIPDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAATWHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCRGGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLGAEVEVCHVVAVGAKSQSLS*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Estimated MW of expressed protein : 135096.34 Daltons
All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.