This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
BRSS, CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, USPL2
Plasmid
pGEX6P-1-CYLD
Parent Plasmid
pGEX6P1
Expressed
GST-CYLD
DU Number
DU1745
Genbank
NP_001035814.1
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCagttcaggcttatggagccaagaaaaagtcacttcaccctactg
ggaagagcggattttttacttgcttcttcaagaatgcagcgttacagaca
aacaaacacaaaagctccttaaagtaccgaagggaagtataggacagtat
attcaagatcgttctgtggggcattcaaggattccttctgcaaaaggcaa
gaaaaatcagattggattaaaaattctagagcaacctcatgcagttctct
ttgttgatgaaaaggatgttgtagagataaatgaaaagttcacagagtta
cttttggcaattaccaattgtgaggagaggttcagcctgtttaaaaacag
aaacagactaagtaaaggcctccaaatagacgtgggctgtcctgtgaaag
tacagctgagatctggggaagaaaaatttcctggagttgtacgcttcaga
ggacccctgttagcagagaggacagtctccggaatattctttggagttga
attgctggaagaaggtcgtggtcaaggtttcactgacggggtgtaccaag
ggaaacagctttttcagtgtgatgaagattgtggcgtgtttgttgcattg
gacaagctagaactcatagaagatgatgacactgcattggaaagtgatta
cgcaggtcctggggacacaatgcaggtcgaacttcctcctttggaaataa
actccagagtttctttgaaggttggagaaacaatagaatctggaacagtt
atattctgtgatgttttgccaggaaaagaaagcttaggatattttgttgg
tgtggacatggataaccctattggcaactgggatggaagatttgatggag
tgcagctttgtagttttgcgtgtgttgaaagtacaattctattgcacatc
aatgatatcatcccagagagtgtgacgcaggaaaggaggcctcccaaact
tgcctttatgtcaagaggtgttggggacaaaggttcatccagtcataata
aaccaaaggctacaggatctacctcagaccctggaaatagaaacagatct
gaattattttataccttaaatgggtcttctgttgactcacaaccacaatc
caaatcaaaaaatacatggtacattgatgaagttgcagaagaccctgcaa
aatctcttacagagatatctacagactttgaccgttcttcaccaccactc
cagcctcctcctgtgaactcactgaccaccgagaacagattccactcttt
accattcagtctcaccaagatgcccaataccaatggaagtattggccaca
gtccactttctctgtcagcccagtctgtaatggaagagctaaacactgca
cccgtccaagagagtccacccttggccatgcctcctgggaactcacatgg
tctagaagtgggctcattggctgaagttaaggagaaccctcctttctatg
gggtaatccgttggatcggtcagccaccaggactgaatgaagtgctcgct
ggactggaactggaagatgagtgtgcaggctgtacggatggaaccttcag
aggcactcggtatttcacctgtgccctgaagaaggcgctgtttgtgaaac
tgaagagctgcaggcctgactctaggtttgcatcattgcagccggtttcc
aatcagattgagcgctgtaactctttagcatttggaggctacttaagtga
agtagtagaagaaaatactccaccaaaaatggaaaaagaaggcttggaga
taatgattgggaagaagaaaggcatccagggtcattacaattcttgttac
ttagactcaaccttattctgcttatttgcttttagttctgttctggacac
tgtgttacttagacccaaagaaaagaacgatgtagaatattatagtgaaa
cccaagagctactggggacagaaattgttaatcctctgagaatatatgga
tatgtgtgtgccacaaaaattatgaaactgaggaaaatacttgaaaaggt
ggaggctgcatcaggatttacctctgaagaaaaagatcctgaggaattct
tgaatattctgtttcatcatattttaagggtagaacctttgctaaaaata
agatcagcaggtcaaaaggtacaagattgttacttctatcaaatttttat
ggaaaaaaatgagaaagttggcgttcccacaattcagcagttgttagaat
ggtcttttatcaacagtaacctgaaatttgcagaggcaccatcatgtctg
attattcagatgcctcgatttggaaaagactttaaactatttaaaaaaat
ttttccttctctggaattaaatataacagatttacttgaagacactccca
gacagtgccggatatgtggagggcttgcaatgtatgagtgtagagaatgc
tacgacgatccggacatctcagctggaaaaatcaagcagttttgtaaaac
ctgcaacactcaagtccaccttcatccgaagaggctgaatcataaatata
acccagtgtcacttcccaaagacttacccgactgggactggagacacggc
tgcatcccttgccagaatatggagttatttgctgttctctgcatagaaac
aagccactatgttgcttttgtgaagtatgggaaggacgattctgcctggc
tcttctttgacagcatggccgatcgggatggtggtcagaatggcttcaac
attcctcaagtcaccccatgcccagaagtaggagagtacttgaagatgtc
tctggaagacctgcattccttggactccaggagaatccaaggctgtgcac
gaagactgctttgtgatgcatatatgtgcatgtaccagagtccaacaatg
agtttgtacaaataaGGATCC
ggaagagcggattttttacttgcttcttcaagaatgcagcgttacagaca
aacaaacacaaaagctccttaaagtaccgaagggaagtataggacagtat
attcaagatcgttctgtggggcattcaaggattccttctgcaaaaggcaa
gaaaaatcagattggattaaaaattctagagcaacctcatgcagttctct
ttgttgatgaaaaggatgttgtagagataaatgaaaagttcacagagtta
cttttggcaattaccaattgtgaggagaggttcagcctgtttaaaaacag
aaacagactaagtaaaggcctccaaatagacgtgggctgtcctgtgaaag
tacagctgagatctggggaagaaaaatttcctggagttgtacgcttcaga
ggacccctgttagcagagaggacagtctccggaatattctttggagttga
attgctggaagaaggtcgtggtcaaggtttcactgacggggtgtaccaag
ggaaacagctttttcagtgtgatgaagattgtggcgtgtttgttgcattg
gacaagctagaactcatagaagatgatgacactgcattggaaagtgatta
cgcaggtcctggggacacaatgcaggtcgaacttcctcctttggaaataa
actccagagtttctttgaaggttggagaaacaatagaatctggaacagtt
atattctgtgatgttttgccaggaaaagaaagcttaggatattttgttgg
tgtggacatggataaccctattggcaactgggatggaagatttgatggag
tgcagctttgtagttttgcgtgtgttgaaagtacaattctattgcacatc
aatgatatcatcccagagagtgtgacgcaggaaaggaggcctcccaaact
tgcctttatgtcaagaggtgttggggacaaaggttcatccagtcataata
aaccaaaggctacaggatctacctcagaccctggaaatagaaacagatct
gaattattttataccttaaatgggtcttctgttgactcacaaccacaatc
caaatcaaaaaatacatggtacattgatgaagttgcagaagaccctgcaa
aatctcttacagagatatctacagactttgaccgttcttcaccaccactc
cagcctcctcctgtgaactcactgaccaccgagaacagattccactcttt
accattcagtctcaccaagatgcccaataccaatggaagtattggccaca
gtccactttctctgtcagcccagtctgtaatggaagagctaaacactgca
cccgtccaagagagtccacccttggccatgcctcctgggaactcacatgg
tctagaagtgggctcattggctgaagttaaggagaaccctcctttctatg
gggtaatccgttggatcggtcagccaccaggactgaatgaagtgctcgct
ggactggaactggaagatgagtgtgcaggctgtacggatggaaccttcag
aggcactcggtatttcacctgtgccctgaagaaggcgctgtttgtgaaac
tgaagagctgcaggcctgactctaggtttgcatcattgcagccggtttcc
aatcagattgagcgctgtaactctttagcatttggaggctacttaagtga
agtagtagaagaaaatactccaccaaaaatggaaaaagaaggcttggaga
taatgattgggaagaagaaaggcatccagggtcattacaattcttgttac
ttagactcaaccttattctgcttatttgcttttagttctgttctggacac
tgtgttacttagacccaaagaaaagaacgatgtagaatattatagtgaaa
cccaagagctactggggacagaaattgttaatcctctgagaatatatgga
tatgtgtgtgccacaaaaattatgaaactgaggaaaatacttgaaaaggt
ggaggctgcatcaggatttacctctgaagaaaaagatcctgaggaattct
tgaatattctgtttcatcatattttaagggtagaacctttgctaaaaata
agatcagcaggtcaaaaggtacaagattgttacttctatcaaatttttat
ggaaaaaaatgagaaagttggcgttcccacaattcagcagttgttagaat
ggtcttttatcaacagtaacctgaaatttgcagaggcaccatcatgtctg
attattcagatgcctcgatttggaaaagactttaaactatttaaaaaaat
ttttccttctctggaattaaatataacagatttacttgaagacactccca
gacagtgccggatatgtggagggcttgcaatgtatgagtgtagagaatgc
tacgacgatccggacatctcagctggaaaaatcaagcagttttgtaaaac
ctgcaacactcaagtccaccttcatccgaagaggctgaatcataaatata
acccagtgtcacttcccaaagacttacccgactgggactggagacacggc
tgcatcccttgccagaatatggagttatttgctgttctctgcatagaaac
aagccactatgttgcttttgtgaagtatgggaaggacgattctgcctggc
tcttctttgacagcatggccgatcgggatggtggtcagaatggcttcaac
attcctcaagtcaccccatgcccagaagtaggagagtacttgaagatgtc
tctggaagacctgcattccttggactccaggagaatccaaggctgtgcac
gaagactgctttgtgatgcatatatgtgcatgtaccagagtccaacaatg
agtttgtacaaataaGGATCC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSSGLWSQEKVTSPYWEERI
FYLLLQECSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRIPSAKGKKNQI
GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERFSLFKNRNRLS
KGLQIDVGCPVKVQLRSGEEKFPGVVRFRGPLLAERTVSGIFFGVELLEE
GRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDTALESDYAGPG
DTMQVELPPLEINSRVSLKVGETIESGTVIFCDVLPGKESLGYFVGVDMD
NPIGNWDGRFDGVQLCSFACVESTILLHINDIIPESVTQERRPPKLAFMS
RGVGDKGSSSHNKPKATGSTSDPGNRNRSELFYTLNGSSVDSQPQSKSKN
TWYIDEVAEDPAKSLTEISTDFDRSSPPLQPPPVNSLTTENRFHSLPFSL
TKMPNTNGSIGHSPLSLSAQSVMEELNTAPVQESPPLAMPPGNSHGLEVG
SLAEVKENPPFYGVIRWIGQPPGLNEVLAGLELEDECAGCTDGTFRGTRY
FTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAFGGYLSEVVEE
NTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSVLDTVLLR
PKEKNDVEYYSETQELLGTEIVNPLRIYGYVCATKIMKLRKILEKVEAAS
GFTSEEKDPEEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNE
KVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSL
ELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCNTQ
VHLHPKRLNHKYNPVSLPKDLPDWDWRHGCIPCQNMELFAVLCIETSHYV
AFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDL
HSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSSGLWSQEKVTSPYWEERI
FYLLLQECSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRIPSAKGKKNQI
GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERFSLFKNRNRLS
KGLQIDVGCPVKVQLRSGEEKFPGVVRFRGPLLAERTVSGIFFGVELLEE
GRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDTALESDYAGPG
DTMQVELPPLEINSRVSLKVGETIESGTVIFCDVLPGKESLGYFVGVDMD
NPIGNWDGRFDGVQLCSFACVESTILLHINDIIPESVTQERRPPKLAFMS
RGVGDKGSSSHNKPKATGSTSDPGNRNRSELFYTLNGSSVDSQPQSKSKN
TWYIDEVAEDPAKSLTEISTDFDRSSPPLQPPPVNSLTTENRFHSLPFSL
TKMPNTNGSIGHSPLSLSAQSVMEELNTAPVQESPPLAMPPGNSHGLEVG
SLAEVKENPPFYGVIRWIGQPPGLNEVLAGLELEDECAGCTDGTFRGTRY
FTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAFGGYLSEVVEE
NTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSVLDTVLLR
PKEKNDVEYYSETQELLGTEIVNPLRIYGYVCATKIMKLRKILEKVEAAS
GFTSEEKDPEEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNE
KVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSL
ELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCNTQ
VHLHPKRLNHKYNPVSLPKDLPDWDWRHGCIPCQNMELFAVLCIETSHYV
AFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDL
HSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK*
Vector Type
Bacterial
Antibiotic
Amp
Comments
contains R641G CF NCBI ref
100042 R 1000 1001 1047 19007
Download datasheet