This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Synonyms
UBA3, Ubiquitin-Activating Enzyme E1 Homolog
Plasmid
pOPC UBA3 6His, GST APPBP1
Parent Plasmid
pOPC
Expressed
UBA3 6His, GST APPBP1
DU Number
DU61958
Genbank
NP_003959.3, NP_003896
Tag
GST
Species
Human
Sequence of Insert
View Sequence
DNA sequence of UBA3 insert
TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGGA
TGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGG
AACCATGTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCTGA
TTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGTAAAG
TTCTAGTCATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTG
GCCTTGTCTGGTTTTAGACAGATTCATGTTATAGATATGGACACTATAGA
TGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATTGGAA
GACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAAT
TGCAATGTAGTTCCACATTTCAACAAGATTCAAGATTTTAACGACACTTT
CTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATCGCCA
GAAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGT
GTCTTAGATCCAAGCTCCATTGTCCCTTTGATAGATGGGGGGACAGAAGG
TTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAATGACTGCTTGTATCG
AATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACC
ATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGAT
GTTGCAGTGGCCTAAGGAGCAGCCTTTTGGAGAAGGGGTTCCATTAGATG
GAGATGATCCTGAACATATACAATGGATTTTCCAAAAATCCCTAGAGAGA
GCATCACAATATAATATTAGGGGTGTTACGTATAGGCTCACTCAAGGGGT
AGTAAAAAGAATCATTCCTGCAGTAGCTTCCACAAATGCAGTCATTGCAG
CTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAAGTGCATACATTCCC
TTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACAC
ATTTGAAGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTC
AAAATATTCAGTTTTCTCCATCAGCTAAACTACAGGAGGTTTTGGATTAT
CTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCAGCCATCACAGCCAC
CCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCTATTG
AAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAAGAATTGGGGCTT
GTTGATGGACAAGAACTGGCGGTTGCTGATGTCACCACCCCACAGACTGT
ACTATTCAAACTTCATTTTACTTCTCATCATCACCATCACCATTAAGGAT
CC
DNA sequence of GST APPBP1
CAATTGAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCC
TATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTC
TTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGAT
GAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCC
CAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGG
CCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCA
AAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAG
ATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAG
TTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGT
TTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTT
CATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCC
TGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATC
CCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTT
GCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGG
ATCTGGTTCCGCGTGGATCCATGGCGCAGCTGGGAAAGCTGCTCAAGGAG
CAGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCATGGGCAAGAGGC
TTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTG
AAATTCTTAAAAACTTGGTACTACCAGGTATTGGTTCGTTTACAATTATT
GATGGAAATCAGGTCAGCGGAGAAGATGCTGGAAACAATTTCTTCCTTCA
AAGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTAC
AAGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAA
AACCTTCTAGACAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGT
TGCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCT
GGAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGT
TATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGA
TAATGCATTAGAGGATCTACGACTAGATAAGCCATTTCCTGAACTGAGAG
AACATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAGGACCACAGT
CATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATAG
TGAAACAAATGGACGAATACCTAAAACGTATAAAGAAAAAGAGGACTTCA
GAGATTTGATTAGACAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAA
GATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAA
TACAACTCAGATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCT
GCATAAATATCACCAAACAGACTCCATCATTTTGGATTTTAGCTCGTGCC
TTAAAGGAATTTGTGGCCAAAGAGGGTCAAGGAAATTTACCTGTTCGAGG
CACAATTCCTGATATGATTGCAGATTCAGGCAAATATATAAAACTGCAAA
ACGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCAT
GTTGCCAAATTGCTGCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGA
GAAAGAATTAAAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAA
GATGTCGATCCTTAGCTGAAGAATATGGTTTGGATACAATTAACAAGGAT
GAAATTATTTCTAGCATGGACAATCCAGATAATGAAATAGTGTTGTACTT
AATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGTAGATATCCAG
GAGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGT
CTCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGA
TTATGTCCACGAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTG
CTGCATTCTTGGGGGGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACC
AAACAATTTGTAATTTTTAATAATACTTACATTTACAGTGGCATGTCACA
AACTTCAGCAACTTTCCAGTTGTAGAAGCTT
TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGGA
TGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGG
AACCATGTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCTGA
TTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGTAAAG
TTCTAGTCATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTG
GCCTTGTCTGGTTTTAGACAGATTCATGTTATAGATATGGACACTATAGA
TGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATTGGAA
GACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAAT
TGCAATGTAGTTCCACATTTCAACAAGATTCAAGATTTTAACGACACTTT
CTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATCGCCA
GAAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGT
GTCTTAGATCCAAGCTCCATTGTCCCTTTGATAGATGGGGGGACAGAAGG
TTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAATGACTGCTTGTATCG
AATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACC
ATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGAT
GTTGCAGTGGCCTAAGGAGCAGCCTTTTGGAGAAGGGGTTCCATTAGATG
GAGATGATCCTGAACATATACAATGGATTTTCCAAAAATCCCTAGAGAGA
GCATCACAATATAATATTAGGGGTGTTACGTATAGGCTCACTCAAGGGGT
AGTAAAAAGAATCATTCCTGCAGTAGCTTCCACAAATGCAGTCATTGCAG
CTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAAGTGCATACATTCCC
TTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACAC
ATTTGAAGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTC
AAAATATTCAGTTTTCTCCATCAGCTAAACTACAGGAGGTTTTGGATTAT
CTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCAGCCATCACAGCCAC
CCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCTATTG
AAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAAGAATTGGGGCTT
GTTGATGGACAAGAACTGGCGGTTGCTGATGTCACCACCCCACAGACTGT
ACTATTCAAACTTCATTTTACTTCTCATCATCACCATCACCATTAAGGAT
CC
DNA sequence of GST APPBP1
CAATTGAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCC
TATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTC
TTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGAT
GAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCC
CAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGG
CCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCA
AAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAG
ATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAG
TTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGT
TTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTT
CATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCC
TGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATC
CCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTT
GCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGG
ATCTGGTTCCGCGTGGATCCATGGCGCAGCTGGGAAAGCTGCTCAAGGAG
CAGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCATGGGCAAGAGGC
TTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTG
AAATTCTTAAAAACTTGGTACTACCAGGTATTGGTTCGTTTACAATTATT
GATGGAAATCAGGTCAGCGGAGAAGATGCTGGAAACAATTTCTTCCTTCA
AAGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTAC
AAGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAA
AACCTTCTAGACAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGT
TGCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCT
GGAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGT
TATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGA
TAATGCATTAGAGGATCTACGACTAGATAAGCCATTTCCTGAACTGAGAG
AACATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAGGACCACAGT
CATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATAG
TGAAACAAATGGACGAATACCTAAAACGTATAAAGAAAAAGAGGACTTCA
GAGATTTGATTAGACAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAA
GATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAA
TACAACTCAGATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCT
GCATAAATATCACCAAACAGACTCCATCATTTTGGATTTTAGCTCGTGCC
TTAAAGGAATTTGTGGCCAAAGAGGGTCAAGGAAATTTACCTGTTCGAGG
CACAATTCCTGATATGATTGCAGATTCAGGCAAATATATAAAACTGCAAA
ACGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCAT
GTTGCCAAATTGCTGCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGA
GAAAGAATTAAAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAA
GATGTCGATCCTTAGCTGAAGAATATGGTTTGGATACAATTAACAAGGAT
GAAATTATTTCTAGCATGGACAATCCAGATAATGAAATAGTGTTGTACTT
AATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGTAGATATCCAG
GAGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGT
CTCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGA
TTATGTCCACGAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTG
CTGCATTCTTGGGGGGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACC
AAACAATTTGTAATTTTTAATAATACTTACATTTACAGTGGCATGTCACA
AACTTCAGCAACTTTCCAGTTGTAGAAGCTT
Amino Acid Sequence
View Sequence
Protein sequence of UBA3
MAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCK
VLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIG
RPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIA
RRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACI
ECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLD
GDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIA
AVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLP
QNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSI
EERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTSHHHHHH*
Protein sequence of GST APPBP1
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMAQLGKLLKEQKYDRQLRLWGDHG
QEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNF
FLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFT
VVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIES
HPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQ
WYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNT
ALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLP
VRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPES
ISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIV
LYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMV
KDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSG
MSQTSATFQL*
MAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCK
VLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIG
RPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIA
RRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACI
ECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLD
GDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIA
AVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLP
QNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSI
EERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTSHHHHHH*
Protein sequence of GST APPBP1
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMAQLGKLLKEQKYDRQLRLWGDHG
QEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNF
FLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFT
VVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIES
HPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQ
WYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNT
ALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLP
VRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPES
ISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIV
LYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMV
KDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSG
MSQTSATFQL*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Correction of DU32498 to add Lac op, LacI and correct premature ATG.
Estimated MW of UBA3 : 50163 Daltons
Estimated MW of GST APPBP1 : 86536 Daltons