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Synonyms
SHANK-associated RH domain interactor, DKFZp434N1923, SIPL1, hSIPL1, sharpin, shank-associated RH domain-interacting protein, shank-interacting protein-like 1
Plasmid
pGEX6P-1-Sharpin Transcript Variant 1
Parent Plasmid
pGEX6P1
Expressed
GST Sharpin
DU Number
DU65992
Genbank
NM_030974.4 → NP_112236.3
Tag
GST
Species
Human
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFINAPDRPGCEMCSTQRPCTWDPLAAAST*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
RT-PCR product comprising C-terminal 260bp section (comprising NZF domain missing from 32976) flanked Xho-Not1 (using native Xho1 site at nt 903) ligated to Xho-Not cut 32976 to build TV1
Silent change compared to NCBI ref at Arg52 CGG>AGG