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Plasmid
pBabeD puro FLAG-BFP-GABARAPL2 KO BamHI
Parent Plasmid
pBabeD puro FLAG
Expressed
FLAG-BFP-GABARAPL2
DU Number
DU65062
Genbank
NP_009216.1 NM_007285.6 GI:27374999
Tag
FLAG
BFP
Species
Human
Sequence of Insert
View Sequence
agatctATGAGCGAGCTGATTAAGGAGAACATGCACATGAAGCTGTACAT
GGAGGGCACCGTGGACAACCATCACTTCAAGTGCACATCCGAGGGCGAAG
GCAAGCCCTACGAGGGCACCCAGACCATGAGAATCAAGGTGGTCGAGGGC
GGCCCTCTCCCCTTCGCCTTCGACATCCTGGCTACTAGCTTCCTCTACGG
CAGCAAGACCTTCATCAACCACACCCAGGGCATCCCCGACTTCTTCAAGC
AGTCCTTCCCTGAGGGCTTCACATGGGAGAGAGTCACCACATACGAAGAC
GGGGGCGTGCTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCT
CATCTACAACGTCAAGATCAGAGGGGTGAACTTCACATCCAACGGCCCTG
TGATGCAGAAGAAAACACTCGGCTGGGAGGCCTTCACCGAGACGCTGTAC
CCCGCTGACGGCGGCCTGGAAGGCAGAAACGACATGGCCCTGAAGCTCGT
GGGCGGGAGCCATCTGATCGCAAACATCAAGACCACATATAGATCCAAGA
AACCCGCTAAGAACCTCAAGATGCCTGGCGTCTACTATGTGGACTACAGA
CTGGAAAGAATCAAGGAGGCCAACAACGAGACCTACGTCGAGCAGCACGA
GGTGGCAGTGGCCAGATACTGCGACCTCCCTAGCAAACTGGGGCACAAGC
TTAATggatcc
GGAGGGCACCGTGGACAACCATCACTTCAAGTGCACATCCGAGGGCGAAG
GCAAGCCCTACGAGGGCACCCAGACCATGAGAATCAAGGTGGTCGAGGGC
GGCCCTCTCCCCTTCGCCTTCGACATCCTGGCTACTAGCTTCCTCTACGG
CAGCAAGACCTTCATCAACCACACCCAGGGCATCCCCGACTTCTTCAAGC
AGTCCTTCCCTGAGGGCTTCACATGGGAGAGAGTCACCACATACGAAGAC
GGGGGCGTGCTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCT
CATCTACAACGTCAAGATCAGAGGGGTGAACTTCACATCCAACGGCCCTG
TGATGCAGAAGAAAACACTCGGCTGGGAGGCCTTCACCGAGACGCTGTAC
CCCGCTGACGGCGGCCTGGAAGGCAGAAACGACATGGCCCTGAAGCTCGT
GGGCGGGAGCCATCTGATCGCAAACATCAAGACCACATATAGATCCAAGA
AACCCGCTAAGAACCTCAAGATGCCTGGCGTCTACTATGTGGACTACAGA
CTGGAAAGAATCAAGGAGGCCAACAACGAGACCTACGTCGAGCAGCACGA
GGTGGCAGTGGCCAGATACTGCGACCTCCCTAGCAAACTGGGGCACAAGC
TTAATggatcc
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQ
TMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFT
WERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLG
WEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKM
PGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLNGSMKWM
FKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSD
ITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGF
LYVAYSGENTFGF*
TMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFT
WERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLG
WEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKM
PGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLNGSMKWM
FKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSD
ITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGF
LYVAYSGENTFGF*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Vector 65044 (Parental 37983). Internal BamHI site (at R67 of GABARAPL2 sequence) knocked out silently
100042 R 1000 1001 1047 19007
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