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Plasmid
pGEX Mindy3 C139A (Drosophila)
Expressed
GST-C3-Mindy3 C139A (Drosophila)
DU Number
DU59950
Genbank
NM_133110.3
Tag
GST
Species
Drosophila Melanogaster
Sequence of Insert
View Sequence
GGATCCATGAACGAGAAAATCGTGCGGGAGCAGCGCGGCGGCGAAGATTCCCCATCCTCCGTCTCGGCGAAAAGTGCAACGGCAGCGGCGTCAGCGTCGACAGCGTCGATGACGTTTGCCTCGGCGGCCTTGGAATCGCACAAGACGACCACCATCACGACCGCCAGCAGCAGCCCTCGCACCAGTGGAGCAAGTGCATCCGCCTCGTCATCCTCTCGATCCGCATCTGCACCTGCATCGTCGTCCAGCCAGCAGGAAAAGCAACCGATGCTGAATGCCACCGATATGCGCGAACTGCGCGAAATCAAGCAGTTGCTGTGGGGCGACAATGTGCGCGAAGATGTATTCAAGCGTTGGTCTCAAGGATTCGAGTTTAGTAAAGTGGAACCGTCTGCATTGGTGCAGAAGCAGGGCGGTCCTGCTGCTGTGATAGCGCCGGTGCAGGCGTACTTGCTTAAGATTATTATCATGGACTTGCCAGGCATCAAGCTCTCGGAGATTTCCCTCGACAAATCGCAGAACCTGCTGATCCAGGCATTGTGTGACATCCTGAAGAATTGTCGGGCGCCGCGGTACCGCATCGTCCATCTCTTGCGCCGACGAGGAAACGCTACAGAGGCTGGATCAACCAAAAAGCGCTCACCGGCTGGCGAGGAAGAATCTGCTTTAGCTGGCCAAGCGGCAGGATCTTCCGAAGAGGTGGAGGAGGCGGCAGAAGCCACTCCCGCCTCGGTCAGCAAGTTGTCCCAGGCCCTGCAGCTCGAACACGATATGCACCAGGAACTGTCGCCGGATGAGTTCCACGAGCGCCTGCACACGCTCCACTTTAAGAATATCGCAGCCGTGGCCCGCTACTATATGGAGAATTACGACCAGCTGGCGCACACATACGGCGTGCTGCTGTTCATGTACTCGGTGTTCCTCACCAAGGGCTTGGAGCTGGTTGCGGCCGATATATCGGACACGTCGGAGCCCCTGATACACAGCACATACGGCTATGGCGGCCAGTCGCTTATTAACCTGATGCTAACTGGGCGAGCAGTTGCCCATGTCTGGGATAATGAGCAGGACGTTGGCGGACTGAAGCTGCGTGGCATCTGTGAGCAGAGCGACATCGGCTTTATAACACTGATGGAGGAAATGCGTTACTGCACGGTGGGCTCCTTCTTCAAGAACCCACGCTATCCCGTATGGGTGATGGGGTCCGATACGCATTTAACCGTTCTGTTCAGCAACGAAAAGAGGCTTGTCTCACCAGAGACGCCCTCGGAAACTGGTCGTCGGATCTTCAAATCGTACGACCCGGAGGGCAACAATTTCATATCGACCACAATGCTGCGCGAAGTTCTCATCGCCTTGAATCTGGTCAGCGAGCCGGCCTATGTCGCCCTCATGCAGAAGCGTCTTGATCCGGAGAACTTGGGCATTATACTGCTAAATGCGTTCATGGACGAGTTCTTCCCGCTGGAGAGCCGCTCCACGCCGGATACGTTCGAGCTGATGCACTACAACGGCATACCGGGCTCCAATGAGAACAACAAGGTGCGCTACTATTGCGGCACGGCCATTTTGCTGGAAGGCGATCTGAAGTCGGTCTGCACCTCGAACCCGATGGTCACCTGCCTGCAGACCAAGTGGCCAAATATCGAAATAAACTGGCACGACGGCCATATGCCCTCCTTGAATTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNEKIVREQRGGEDSPSSVSAKSATAAASASTASMTFASAALESHKTTTITTASSSPRTSGASASASSSSRSASAPASSSSQQEKQPMLNATDMRELREIKQLLWGDNVREDVFKRWSQGFEFSKVEPSALVQKQGGPAAVIAPVQAYLLKIIIMDLPGIKLSEISLDKSQNLLIQALCDILKNCRAPRYRIVHLLRRRGNATEAGSTKKRSPAGEEESALAGQAAGSSEEVEEAAEATPASVSKLSQALQLEHDMHQELSPDEFHERLHTLHFKNIAAVARYYMENYDQLAHTYGVLLFMYSVFLTKGLELVAADISDTSEPLIHSTYGYGGQSLINLMLTGRAVAHVWDNEQDVGGLKLRGICEQSDIGFITLMEEMRYCTVGSFFKNPRYPVWVMGSDTHLTVLFSNEKRLVSPETPSETGRRIFKSYDPEGNNFISTTMLREVLIALNLVSEPAYVALMQKRLDPENLGIILLNAFMDEFFPLESRSTPDTFELMHYNGIPGSNENNKVRYYCGTAILLEGDLKSVCTSNPMVTCLQTKWPNIEINWHDGHMPSLN*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please mutate C139 to alanine in 59102. Cheers!
100042 R 1000 1001 1047 19007
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