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Plasmid
pCAGGS puro 3FLAG mouse USP26 C295S
Parent Plasmid
pCAGGS puro 3FLAG
Expressed
3FLAG mouse USP26 C295S
DU Number
DU63654
Genbank
AF285570.1 GI:13603822
Tag
3FLAG
Species
Mouse
Sequence of Insert
View Sequence
GAATTCGCCACCATGGAGCCCATATTGATAAATGCTCAAGTCCAGATGTGGAGTGCAAAGGCAGGAATGTCCAAGTCAAGAAATGCACTCATTGAAACATGTGTAGGAAAACGAGAAGTTAAACTTATTCTCTATTTCAGCACTGGAAAGATTAAGACTTTGCAACTACACGATAATATTAAAAGTGTGGTCCTTCAAACCTATGGCGAAGACCAGAATTACCTACATTTGACTTTTAAAAATAATGATTTCTTGTTTGTTGAGAAACTCACCACCACAGATGCCAGAAGACTGAAGAGATTTCTAGACAAAACCTCTCAAGGTAGTATTCGGCCAGCCAGAAGTGATGAGAGATGTGGTGAGCCTAGCACAAGTGCACAGGAGTTGAATGGCTCTGGAAGTTCATGTGAAACAAATAGTGAGTGCTTTGAATCACCCAAAGAAAGTGAAATGTGCATGTTTCGTGAGTTGTCTTTGCTTCCATCCTCATCAACCTTTCTTCACAATGTAGGATTATTAGAAAACCAATTCATAAAGAGGAAAAGATTTTTCTCTGATTTAGCAAAAAATGAAAAACAGAGCAACCTGAAGGACAGTATCAGGGACTTTGAGGCAAATTTAGTGGTGTGTATCTCTAATGAAAAGGGAAAAGAAAGGAATGTAAGAGAAGTAGACATCAGTAAGCCAGGGTTTGGATTTCCATTTGAGACCAACTATCCTGAAGATAGTGGTGTGGATGTTCGTGATCTTAATGATCTCATTACAAAATTATTTTCTCCAGTTCTGTTGGAAACACACTGTATTGAGAACGGCCTAGAGTGGCATGAATATATGAAGACATACTTGCTTTACCCAGAGAAATTGTGGCAAGGCCTGCCTAATGTGGGAAACACCAGCTATATAAATGTTGTATTACAGTCTCTATGCTCAATACCACTGTTTATTAATGATTTATTCAACCAGGGTTTCCCATGGATTAAAGCTCCCAAAGATGATTTTAACATGCTCTTGATGCAACTGCTTGTTTTGAAAGATATTTACAACGCAAGATTTAGACAGAAGTTACTTATAGGTATTACAAAAGCCCTCCCCATATTTGGAGAGATATTTGCTGTTGACAGGCAGAATGATGCTCATGAGTTTTTAAGTCTCTGTTTAGTTCAGTTGAAGGAGACTTTCCAAAGAGTAACCATGATGTGGCAGTCTGAAAATGATTCGGGGGATTTTTACTTACTTAAAGACATTTTTGCTGATTATGCTACTATCAACAAAATGCCCGTTTGTCCTGTTACCAATAATTTTGAATTTGAGTTGCTAAGCTCCATTTTTTGTAAAGCTTGTGGCCTGACTCTTTTTAAGGGAGAACCAAGTAGATACCTTTCTATCAACATTCCCCAAGGAGGGAAAGACATGTCCATCCAGTCCACTTTAGATCTTTTCTTTAGTGCAGAGGAGCTTGAGCATAGGTGTGAAAAGTGTTTGTACAACAAATCTGTTTCATTTCACAGGTTTGGCCGGCTACCCAGGGTAATTATTGTTCATCTGAAACGCTATCACTTTAATGAGTCATGGGTAATGAAGAAGGATGAGCGGCCCATCCTTGTTTCCAAATACTTAAGGCTGTCTTGTCACTGTAGCAAAAGCACAAAACCGCCCCCACCCCTTCGCCCAGGTGAACATGTTAAGAATCTTGACTTATTAAAACCCCTTGAAGTGTTGGGTTCCGAAATACTCAAATTGCCTTTTAATTCAGTGAGGACCTCTAGATCCAAGGGTTTCGAAACTATAAACATCACATCAAACAGGGAGTCAGAAGCACAAAGTGGGAAAAGAGTCTCTGAAGTGTTGAGTGGAAAAGTGCAGCAGGAAAATTCAGGGAAAGGTGACACAGCACATATAGTTGGGTCAGAACTTACAAAGGAGACTGAGAAACTCAAGAAACATGAGGAAGAGCATAGACCCAGTGATTTAGATTCTGGTAGTATCAGGGAGGCCCAAAAGTACCAACAGGCTGAGAAATGTAACGAAGGGAGAAGTGATAAGCAGATTTCCCTAGAGGCACTTACTCAAAGCCGTCCAAAACCAATCTCCCAGGAACAGACAGAAAACCTTGGGAAAACTACACTGTCACATACCCAGGATAGTAGTCAGAGTTCACAGAGCTCATCAGATTCCAGTAAGAGCTCCCGATGCAGTGATGATCTCGATAAGAAGGCAAAGCCTACACGCAAGGTGGATCCAACAAAGTTTAATAAAAAAGAAGATAATGTTTACAGGCTTGTTAATATTATCAACCATATTGGGAACAGTCCCAATGGAGGCCACTACATCAATGATGCCTTTGACTTCAAGAGGCAGAGTTGGTTCACTTATAGTGATCTACATGTAACAAGAACCCAAGAGGACTTTGTATATAGGGGTCGGAGTTCTACTGGGTATGTCTTCTTTTACATGCATAATGATATATTTGAAGAGCTCTTGGCAAAGGAAACTCAGTCTACCAGCACATCCAAGGGTTAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDHDGDYKDHDIDYKDDDDKGSPEFATMEPILINAQVQMWSAKAGMSKSRNALIETCVGKREVKLILYFSTGKIKTLQLHDNIKSVVLQTYGEDQNY
LHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSQGSIRPARSDERCGEPSTSAQELNGSGSSCETNSECFESPKESEMCMFRELSLLPSSSTFLHNVGLLE
NQFIKRKRFFSDLAKNEKQSNLKDSIRDFEANLVVCISNEKGKERNVREVDISKPGFGFPFETNYPEDSGVDVRDLNDLITKLFSPVLLETHCIENGLEW
HEYMKTYLLYPEKLWQGLPNVGNTSYINVVLQSLCSIPLFINDLFNQGFPWIKAPKDDFNMLLMQLLVLKDIYNARFRQKLLIGITKALPIFGEIFAVDR
QNDAHEFLSLCLVQLKETFQRVTMMWQSENDSGDFYLLKDIFADYATINKMPVCPVTNNFEFELLSSIFCKACGLTLFKGEPSRYLSINIPQGGKDMSIQ
STLDLFFSAEELEHRCEKCLYNKSVSFHRFGRLPRVIIVHLKRYHFNESWVMKKDERPILVSKYLRLSCHCSKSTKPPPPLRPGEHVKNLDLLKPLEVLG
SEILKLPFNSVRTSRSKGFETINITSNRESEAQSGKRVSEVLSGKVQQENSGKGDTAHIVGSELTKETEKLKKHEEEHRPSDLDSGSIREAQKYQQAEKC
NEGRSDKQISLEALTQSRPKPISQEQTENLGKTTLSHTQDSSQSSQSSSDSSKSSRCSDDLDKKAKPTRKVDPTKFNKKEDNVYRLVNIINHIGNSPNGG
HYINDAFDFKRQSWFTYSDLHVTRTQEDFVYRGRSSTGYVFFYMHNDIFEELLAKETQSTSTSKG*
LHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSQGSIRPARSDERCGEPSTSAQELNGSGSSCETNSECFESPKESEMCMFRELSLLPSSSTFLHNVGLLE
NQFIKRKRFFSDLAKNEKQSNLKDSIRDFEANLVVCISNEKGKERNVREVDISKPGFGFPFETNYPEDSGVDVRDLNDLITKLFSPVLLETHCIENGLEW
HEYMKTYLLYPEKLWQGLPNVGNTSYINVVLQSLCSIPLFINDLFNQGFPWIKAPKDDFNMLLMQLLVLKDIYNARFRQKLLIGITKALPIFGEIFAVDR
QNDAHEFLSLCLVQLKETFQRVTMMWQSENDSGDFYLLKDIFADYATINKMPVCPVTNNFEFELLSSIFCKACGLTLFKGEPSRYLSINIPQGGKDMSIQ
STLDLFFSAEELEHRCEKCLYNKSVSFHRFGRLPRVIIVHLKRYHFNESWVMKKDERPILVSKYLRLSCHCSKSTKPPPPLRPGEHVKNLDLLKPLEVLG
SEILKLPFNSVRTSRSKGFETINITSNRESEAQSGKRVSEVLSGKVQQENSGKGDTAHIVGSELTKETEKLKKHEEEHRPSDLDSGSIREAQKYQQAEKC
NEGRSDKQISLEALTQSRPKPISQEQTENLGKTTLSHTQDSSQSSQSSSDSSKSSRCSDDLDKKAKPTRKVDPTKFNKKEDNVYRLVNIINHIGNSPNGG
HYINDAFDFKRQSWFTYSDLHVTRTQEDFVYRGRSSTGYVFFYMHNDIFEELLAKETQSTSTSKG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
catalytically inactive mouse USP26 (C295S) in pCAGGS puro backbone.
100042 R 1000 1001 1047 19007
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