MYCBP2 | Ubiquitylation Resource

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Plasmid

pSc-B-MYCBP2 Met1-K2339 part corrected

Parent Plasmid

pSC-B

DU Number

DU29549

Tag

Myc

Species

Human

Sequence of Insert

View Sequence

GCTAGCATGCCGGTTCCCGACGGCTCCGTGGCTGCTGCGGGGCTGGGGCT
GGGGCTACCCGCCGCGGACTCCCGGGGTCACTACCAGCTGCTGCTGTCAG
GCCGGGCCCTGGCCGACCGCTACCGGAGGATTTATACCGCTGCGCTCAAT
GACAGGGACCAGGGGGGCGGCAGCGCTGGACACCCAGCCTCCAGGAATAA
GAAAATTTTAAATAAGAAGAAATTGAAAAGAAAACAGAAGAGCAAATCAA
AAGTGAAGACAAGAAGCAAGTCTGAAAACTTAGAGAATACAGTAATCATA
CCAGATATCAAACTACATAGCAATCCTTCTGCTTTCAATATTTACTGTAA
TGTACGCCATTGCGTTCTGGAATGGCAGAAGAAGGAAATATCATTGGCAG
CCGCATCTAAGAACTCTGTGCAGAGTGGAGAATCAGATAGTGATGAAGAA
GAGGAATCCAAAGAGCCCCCTATCAAGCTTCCAAAGATTATTGAGGTTGG
CCTTTGTGAAGTTTTTGAATTGATCAAAGAGACACGATTTTCTCATCCAT
CCCTGTGTCTCAGGAGTCTCCAAGCCCTGCTCAACGTGCTGCAGGGCCAG
CAGCCAGAAGGCCTCCAGTCTGAGCCACCTGAGGTCCTAGAGTCTCTCTT
CCAGCTTCTTTTGGAAATCACCGTTCGAAGTACTGGGATGAATGACAGCA
CAGGACAGTCCTTAACAGCACTTTCCTGTGCTTGCCTCTTTAGTCTGGTG
GCTTCTTGGGGAGAAACAGGAAGGACACTTCAGGCCATCTCTGCTATCCT
CACCAACAATGGAAGCCATGCTTGCCAAACTATTCAGGTGCCAACAATTC
TAAATTCGCTACAGAGAAGTGTACAAGCAGTTTTGGTGGGAAAAATTCAA
ATTCAGGACTGGTTTAGTAATGGCATTAAGAAAGCAGCTTTAATGCACAA
GTGGCCATTAAAAGAAATATCTGTTGATGAAGATGACCAATGTCTACTTC
AGAATGATGGATTTTTTCTTTATCTATTATGCAAGGATGGATTATATAAA
ATAGGCTCTGGATACAGTGGAACAGTTAGGGGCCATATATACAATTCTAC
ATCCCGTATTAGAAACAGAAAAGAAAAAAAGTCTTGGTTAGGGTATGCTC
AGGGTTATTTATTATATAGAGATGTGAATAACCACAGCATGACAGCCATA
AGGATAAGCCCTGAAACACTGGAGCAAGATGGTACTGTGATGTTACCAGA
TTGCCACACTGAAGGTCAAAATATTTTATTCACTGATGGAGAATATATTA
ATCAGATAGCTGCTTCAAGAGATGATGGCTTTGTTGTCAGAATATTTGCC
ACAAGCACTGAACCTGTTCTACAGCAAGAATTGCAACTTAAACTGGCTAG
AAAATGCTTACATGCCTGTGGTATCTCACTATTCGATCTGGAAAAGGACT
TGCATATTATAAGTACAGGATTTGATGAGGAGTCAGCAATTCTTGGTGCA
GGACGAGAGTTTGCGCTAATGAAAACAGCAAATGGAAAGATATATTACAC
TGGCAAATACCAGAGTCTTGGAATCAAACAAGGTGGTCCTTCAGCAGGAA
AATGGGTTGAGCTACCAATTACAAAATCTCCAAAGATAGTACACTTCTCA
GTTGGACACGATGGCTCTCACGCCCTTTTAGTTGCAGAAGATGGGAGCAT
ATTCTTTACAGGATCTGCTAGTAAAGGAGAAGATGGAGAATCAACTAAGA
GCAGACGGCAATCCAAACCTTATAAACCTAAAAAGATAATTAAGATGGAA
GGAAAGATTGTGGTATATACAGCCTGCAATAATGGAAGTAGTTCTGTTAT
TTCTAAAGATGGAGAACTCTACATGTTTGGAAAAGATGCCATTTACTCTG
ATAGTTCAAGTTTGGTAACTGATTTGAAGGGCCATTTTGTAACTCAGGTA
GCTATGGGCAAAGCTCACACTTGTGTTTTAATGAAGAATGGAGAGGTGTG
GACATTTGGTGTAAATAATAAAGGACAGTGTGGACGAGATACTGGTGCCA
TGAACCAAGGTGGGAAAGGGTTTGGAGTTGAAAATATGGCAACAGCAATG
GATGAAGACCTGGAAGAAGAACTAGATGAAAAAGATGAGAAGTCTATGAT
GTGCCCTCCAGGCATGCACAAATGGAAGCTGGAGCAGTGCATGGTTTGCA
CTGTCTGTGGAGACTGTACAGGTTATGGAGCCAGCTGTGTCAGTAGTGGA
CGGCCAGACAGAGTCCCCGGAGGGATCTGTGGTTGTGGTTCCGGAGAATC
TGGTTGTGCTGTGTGTGGATGTTGCAAGGCCTGTGCAAGAGAGTTAGATG
GTCAAGAGGCAAGACAAAGAGGAATTCTTGATGCAGTGAAAGAAATGATA
CCTTTAGATCTTCTTTTAGCTGTCCCAGTGCCCGGGGTTAACATTGAAGA
ACACCTTCAGTTACGACAAGAAGAAAAACGGCAACGTGTAATCAGAAGGC
ACAGATTAGAGGAAGGAAGAGATGGAAGTGGAGAAAGAGGCGAAAAGGAT
GCAAGCAAAATCACAACATACCCTCCAGGCTCTGTGCGATTTGACTGTGA
GCTCCGGGCAGTCCAAGTCAGCTGTGGATTTCACCATTCAGTGGTTTTAA
TGGAAAATGGAGATGTCTATACATTTGGTTATGGGCAGCATGGGCAGCTA
GGACATGGAGATGTCAACTCCAGGGGATGTCCCACTCTTGTTCAAGCATT
GCCAGGCCCTAGCACACAAGTCACTGCAGGCAGCAACCATACGGCAGTAC
TTTTAATGGATGGACAGGTCTTCACATTTGGAAGTTTTTCTAAAGGACAA
CTGGGCAGACCAATTTTGGATGTGaCATATTGGAATGCAAAGCCAGCTCC
CATGCCTAACATTGGATCAAAATATGGAAGAAAAGCTACTTGGATAGGTG
CAAGTGGGGACCAAACTTTTTTACGAATTGATGAAGCACTTATTAATTCT
CATGTACTTGCTACATCAGAAATTTTTGCCAGTAAACACATAATAGGCTT
GGTACCTGCTTCTATATCAGAACCTCCTCCATTTAAATGCCTTCTGATAA
ATAAAGTGGATGGGAGTTGTAAAACTTTTAATGACTCAGAACAAGAGGAT
CTGCAAGGATTTGGTGTGTGTCTTGATCCTGTATACGATGTAATTTGGAG
GTTTCGACCAAATACTAGAGAGCTGTGGTGTTACAATGCGGTGGTTGCTG
ATGCCAGGCTTCCCTCTGCAGCAGACATGCAGTCCAGATGTAGTATCCTA
AGTCCTGAACTTGCCTTACCAACAGGATCAAGGGCCCTCACTACCCGATC
TCATGCAGCTTTGCACATTTTAGGTTGTCTTGATACCTTGGCAGCTATGC
AGGACTTAAAAATGGGTGTTGCAAGTACAGAGGAAGAGACTCAAGCAGTA
ATGAAGGTTTATTCTAAAGAAGATTATAGTGTGGTAAACAGGTTTGAAAG
TCATGGAGGAGGCTGGGGTTATTCTGCCCATTCAGTAGAAGCTATACGTT
TCAGTGCCGACACTGATATTTTACTTGGTGGTCTTGGTCTGTTTGGAGGT
AGAGGAGAATATACTGCTAAAATTAAGCTGTTTGAATTGGGTCCTGATGG
AGGAGATCATGAAACTGATGGTGACCTTCTTGCAGAGACTGATGTATTGG
CTTATGACTGTGCTGCTAGAGAAAAATATGCAATGATGTTTGATGAGCCT
GTTCTCCTGCAAGCTGGGTGGTGGTATGTGGCATGGGCCCGAGTGTCAGG
ACCCAGCAGTGACTGTGGATCTCATGGACAGGCATCTATTACCACAGATG
ATGGGGTTGTTTTCCAGTTCAAGAGTTCAAAGAAATCAAATAATGGTACA
GATGTTAATGCGGGTCAGATACCTCAGTTATTATACAGACTTCCAACCAG
TGATGGCAGTGCTTCAAAAGGCAAACAGCAAACCAGTGAACCTGTACACA
TTTTAAAGAGGTCTTTTGCAAGAACTGTCTCAGTGGAATGTTTTGAGTCA
TTGTTGAGTATTCTTCACTGGAGCTGGACCACCTTAGTCTTAGGAGTTGA
AGAACTTAGAGGATTAAAAGGATTCCAGTTCACAGCTACACTCCTAGATT
TAGAGAGACTGCGCTTTGTGGGTACCTGTTGTCTGAGGTTATTGCGTGTC
TATACCTGTGAAATTTACCCAGTGTCAGCTACAGGAAAAGCAGTTGTAGA
AGAAACTAGCAAATTAGCAGAGTGTATTGGAAAAACCAGAACTTTGTTAA
GAAAAATTTTATCAGAAGGAGTTGATCACTGCATGGTGAAATTGGATAAT
GATCCTCAAGGATATCTCAGTCAACCCTTGAGTCTTCTAGAAGCTGTCCT
TCAGGAATGTCATAATACTTTCACTGCCTGCTTTCATTCTTTCTACCCAA
CTCCTGCCTTACAGTGGGCTTGCCTTTGTGATCTGCTGAATTGTTTGGAT
CAGGATATCCAAGAAGCAAACTTCAAGACATCAAGTAGCCGACTCCTTGC
AGCTGTTATGTCAGCTCTGTGTCACACGTCTGTTAAGCTGACTTCCATCT
TCCCGATTGCGTATGATGGAGAAGTATTACTACGATCAATTGTTAAACAA
GTTAGTACAGAGAACGACTCAACACTAGTTCATCGTTTTCCCCTTTTGGT
GGCACATATGGAAAAACTCAGCCAGAGTGAAGAGAATATCTCAGGGATGA
CAAGCTTCCGTGAAGTTCTGGAGAAAATGCTGGTCATTGTTGTGCTACCA
GTCAGGAACAGCCTGAGGAGAGAAAATGAACTCTTCTCCTCCCACCTCGT
CTCTAACACCTGTGGATTACTGGCCAGCATTGTCAGTGAACTGACAGTGT
CAGCCCTGGGATCTGAGGTTGATGGACTTAATTCTCTTCACTCTGTAAAA
GCTAGTGCTAACCGATTTACAAAAACAAGTCAGGGCAGAAGTTGGAACAC
TGGGAACGGGTCCCCTGATGCAATCTGTTTTTCAGTAGACAAACCTGGAA
TAGTTGTGGTTGGTTTCTCTGTCTATGGAGGAGGTGGAATTCATGAATAT
GAATTAGAGGTGTTAGTTGATGATAGTGAACATGCAGGAGATTCAACTCA
TTCCCACAGATGGACATCTCTGGAATTAGTGAAAGGAACGTACACAACGG
ATGACTCACCCAGTGATATAGCTGAGATCAGACTTGACAAAGTGGTTCCT
TTAAAGGAAAATGTTAAATATGCTGTGCGCTTGAGGAACTATGGAAGCCG
TACAGCCAATGGAGATGGAGGAATGACCACAGTTCAGTGCCCTGATGGTG
TGACATTCACATTCAGCACGTGCAGCTTGAGCAGTAACGGCACAAACCAA
ACCAGAGGACAGATCCCACAGATACTCTACTATAGGAGTGAATTTGATGG
AGATTTACAATCCCAACTTCTGAGTAAAGCCAATGAAGAAGATAAAAACT
GTAGCAGAGCATTGTCTGTTGTAAGCACTGTCGTTCGAGCCTCTAAGGAC
CTCCTGCACAGAGCTCTTGCTGTGGATGCTGATGACATTCCAGAACTGCT
GAGTTCTTCCAGTCTGTTTTCCATGCTGCTCCCCCTTATTATAGCCTACA
TAGGACCAGTAGCTGCTGCTATTCCCAAGGTGGCTGTAGAAGTCTTTGGC
CTTGTCCAACAATTGCTTCCGTCAGTTGCCATTTTGAATCAGAAGTATGC
ACCGCCTGCCTTCAACCCTAATCAGTCGACAGATAGCACCACAGGAAACC
AGCCTGAACAGGGCCTCTCTGCTTGTACAACCTCCAGTCACTATGCTGTC
ATAGAGAGTGAGCACCCGTATAAACCTGCCTGTGTGATGCATTACAAGGT
GACATTCCCAGAATGCGTGAGGTGGATGACAATCGAATTTGACCCTCAGT
GTGGTACTGCACAGTCAGAAGATGTCCTTCGTTTGTTGATTCCTGTCAGA
ACTGTTCAGAATTCAGGATATGGACCAAAATTGACATCTGTTCATGAAAA
TCTTAATTCATGGATAGAATTAAAGAAATTTTCAGGATCCTCTGGGTGGC
CTACTATGGTTTTGGTGTTGCCAGGAAATGAGGCCCTTTTTTCATTGGAG
ACTGCATCAGATTATGTGAAAGATGACAAAGCTTCTTTCTATGGTTTTAA
GTGTTTTGCAATTGGATATGAATTTAGCCCTGGACCTGATGAGGGAGTCA
TCCAATTGGAAAAAGAATTAGCCAATCTTGGTGGGGTTTGTGCAGCAGCT
CTGATGAAGAAGGACCTAGCACTTCCTATTGGTAATGAATTAGAAGAAGA
CCTTGAAATTCTTGAGGAGGCTGCATTGCAGGTGTGCAAAACCCATTCTG
GAATTCTTGGAAAGGGTCTAGCTCTTTCTCATTCACCAACTATATTAGAA
GCACTTGAGGGAAATTTACCACTCCAAATCCAAAGCAATGAACAGTCTTT
TCTGGATGATTTTATTGCCTGTGTCCCAGGATCAAGTGGTGGAAGGCTTG
CAAGGTGGCTTCAGCCAGATTCATATGCGGATCCTCAGAAAACATCTTTG
ATCCTGAATAAGGATGATATTCGTTGTGGTTGGCCTACCACCATAACTGT
TCAAACAAAAGACCAGTATGGGGATGTGGTACATGTTCCCAATATGAAGG
TGGAAGTGAAAGCTGTCCCTGTTTCTCAGAAAAAAATGTCTTTACAACAA
GATCAAGCAAAGAAACCTCAAAGGATTCCTGGCAGTCCTGCAGTAACAGC
TGCATCTTCTAATACTGACATGACTTATGGAGGGCTGGCATCACCAAAGC
TAGATGTTTCATATGAACCAATGATAGTGAAGGAAGCTCGATATATTGCC
ATAACAATGATGAAGCTCGAG

Amino Acid Sequence

View Sequence

MPVPDGSVAAAGLGLGLPAADSRGHYQLLLSGRALADRYRRIYTAALNDR
DQGGGSAGHPASRNKKILNKKKLKRKQKSKSKVKTRSKSENLENTVIIPD
IKLHSNPSAFNIYCNVRHCVLEWQKKEISLAAASKNSVQSGESDSDEEEE
SKEPPIKLPKIIEVGLCEVFELIKETRFSHPSLCLRSLQALLNVLQGQQP
EGLQSEPPEVLESLFQLLLEITVRSTGMNDSTGQSLTALSCACLFSLVAS
WGETGRTLQAISAILTNNGSHACQTIQVPTILNSLQRSVQAVLVGKIQIQ
DWFSNGIKKAALMHKWPLKEISVDEDDQCLLQNDGFFLYLLCKDGLYKIG
SGYSGTVRGHIYNSTSRIRNRKEKKSWLGYAQGYLLYRDVNNHSMTAIRI
SPETLEQDGTVMLPDCHTEGQNILFTDGEYINQIAASRDDGFVVRIFATS
TEPVLQQELQLKLARKCLHACGISLFDLEKDLHIISTGFDEESAILGAGR
EFALMKTANGKIYYTGKYQSLGIKQGGPSAGKWVELPITKSPKIVHFSVG
HDGSHALLVAEDGSIFFTGSASKGEDGESTKSRRQSKPYKPKKIIKMEGK
IVVYTACNNGSSSVISKDGELYMFGKDAIYSDSSSLVTDLKGHFVTQVAM
GKAHTCVLMKNGEVWTFGVNNKGQCGRDTGAMNQGGKGFGVENMATAMDE
DLEEELDEKDEKSMMCPPGMHKWKLEQCMVCTVCGDCTGYGASCVSSGRP
DRVPGGICGCGSGESGCAVCGCCKACARELDGQEARQRGILDAVKEMIPL
DLLLAVPVPGVNIEEHLQLRQEEKRQRVIRRHRLEEGRDGSGERGEKDAS
KITTYPPGSVRFDCELRAVQVSCGFHHSVVLMENGDVYTFGYGQHGQLGH
GDVNSRGCPTLVQALPGPSTQVTAGSNHTAVLLMDGQVFTFGSFSKGQLG
RPILDVTYWNAKPAPMPNIGSKYGRKATWIGASGDQTFLRIDEALINSHV
LATSEIFASKHIIGLVPASISEPPPFKCLLINKVDGSCKTFNDSEQEDLQ
GFGVCLDPVYDVIWRFRPNTRELWCYNAVVADARLPSAADMQSRCSILSP
ELALPTGSRALTTRSHAALHILGCLDTLAAMQDLKMGVASTEEETQAVMK
VYSKEDYSVVNRFESHGGGWGYSAHSVEAIRFSADTDILLGGLGLFGGRG
EYTAKIKLFELGPDGGDHETDGDLLAETDVLAYDCAAREKYAMMFDEPVL
LQAGWWYVAWARVSGPSSDCGSHGQASITTDDGVVFQFKSSKKSNNGTDV
NAGQIPQLLYRLPTSDGSASKGKQQTSEPVHILKRSFARTVSVECFESLL
SILHWSWTTLVLGVEELRGLKGFQFTATLLDLERLRFVGTCCLRLLRVYT
CEIYPVSATGKAVVEETSKLAECIGKTRTLLRKILSEGVDHCMVKLDNDP
QGYLSQPLSLLEAVLQECHNTFTACFHSFYPTPALQWACLCDLLNCLDQD
IQEANFKTSSSRLLAAVMSALCHTSVKLTSIFPIAYDGEVLLRSIVKQVS
TENDSTLVHRFPLLVAHMEKLSQSEENISGMTSFREVLEKMLVIVVLPVR
NSLRRENELFSSHLVSNTCGLLASIVSELTVSALGSEVDGLNSLHSVKAS
ANRFTKTSQGRSWNTGNGSPDAICFSVDKPGIVVVGFSVYGGGGIHEYEL
EVLVDDSEHAGDSTHSHRWTSLELVKGTYTTDDSPSDIAEIRLDKVVPLK
ENVKYAVRLRNYGSRTANGDGGMTTVQCPDGVTFTFSTCSLSSNGTNQTR
GQIPQILYYRSEFDGDLQSQLLSKANEEDKNCSRALSVVSTVVRASKDLL
HRALAVDADDIPELLSSSSLFSMLLPLIIAYIGPVAAAIPKVAVEVFGLV
QQLLPSVAILNQKYAPPAFNPNQSTDSTTGNQPEQGLSACTTSSHYAVIE
SEHPYKPACVMHYKVTFPECVRWMTIEFDPQCGTAQSEDVLRLLIPVRTV
QNSGYGPKLTSVHENLNSWIELKKFSGSSGWPTMVLVLPGNEALFSLETA
SDYVKDDKASFYGFKCFAIGYEFSPGPDEGVIQLEKELANLGGVCAAALM
KKDLALPIGNELEEDLEILEEAALQVCKTHSGILGKGLALSHSPTILEAL
EGNLPLQIQSNEQSFLDDFIACVPGSSGGRLARWLQPDSYADPQKTSLIL
NKDDIRCGWPTTITVQTKDQYGDVVHVPNMKVEVKAVPVSQKKMSLQQDQ
AKKPQRIPGSPAVTAASSNTDMTYGGLASPKLDVSYEPMIVKEARYIAIT
MMK

Vector Type

Other

Antibiotic

Amp; Kan

Comments

Non-silent mutations in 29461 precursor have been corrected restoring V323, M715, M1280, V2268 - but there is a new mutation to correct at P993T (with respect to full-length Virdee group sequence)

aa Met1-Lys2339 amplified from 26850 with NW5195/96 with the following changes relative to Virdee group ref sequence (Virdee MYCBP2 ref attached below):
Contains listed SNP A1667V (equates to aa1631 in this intermediate with the deletion listed below) - this SNP equates to A1705V in NM_015057.4

Silent mutations:
K125 (AAA > AAG), Y1096 (TAT > TAC), L1739 (TTG > TTA), C2006 (TGT > TGC),
Clone also contains a deletion comprising G839-K874 of Virdee Group ref sequence.

There is an artificial Xho1 site at nt 6966 (to facilitate downstream joining of the two half lengths of full-length MYCBP2 once all corrections to first half have been made).
outwith the sequences described this clone contains a 5' extension comprising overlap with FRT/TO-MYC Nhe1 and a 3' extension comprising overlap to FRT/TO-MYC Xho1 in DU58354 to join the two half lengths with NEB builder

100042 R 1000 1001 1047 19007

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