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ISG15

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Plasmid
pSc-B-ISG15 (aa1-154) C78S T3C intein overlap
Parent Plasmid
pSC-B
DU Number
DU29472
Species
Human
Sequence of Insert
GCTAGCATGGGTTGCTCTTCCGGCGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATTCCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCACCCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCTGGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATCCGACGAACCTCTGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTGCGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGAGGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGGAGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTGTTCATGAATCTGCGCCTGTGCATCTGCGGAGATGCACTAGT
Amino Acid Sequence
MGCSSGGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRL
AVHPSGVALQDRVPLASQGLGPGSTVLLVVDKSDEPLNILVRNNKGRSST
YEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPL
STVFMNLRLCICGDAL
Vector Type
Other
Antibiotic
Amp; Kan
Comments
GCSSG inserted after Met1 and 3' end overlaps intein (to Spe1 site) and includes T3C mutation. No stop. For cloning into Nhe1 Spe1 sites of pTXB1
100042 R 1000 1001 1047 19007
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