CHFR | Ubiquitylation Resource

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Synonyms

E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains

Plasmid

pS-B-CHFR isoform 1

Parent Plasmid

pS-B

DU Number

DU29278

Genbank

NM_001161344.1 → NP_001154816.1

Species

Human

Sequence of Insert

View Sequence

ggatccATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGAAGCTAACAAGGAAAATGTGTTCCATGGGACCAAAGATACC
TCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTC
GCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCGACGT
CAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCA
GGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCC
TAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCT
CAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAG
GATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGA
CCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAATGCCC
AAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATG
GAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGT
GAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCT
GGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGG
ATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCA
GCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCA
GGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTT
TCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGA
CAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTC
CTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAG
GGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGT
CAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCC
ACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAG
CGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCA
GCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCT
GCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGC
GTGCTGAACAACAACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGC
AACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTGGCTC
TCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACC
GTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCA
GTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCC
GTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCAC
CACGCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAACTA
Agcggccgc

Amino Acid Sequence

View Sequence

MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSF
PSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTG
DVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEANKENVFHGTKDTSG
AGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGR
ERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQ
EDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEE
TLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERIC
KNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSD
EEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGE
PGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAERE
QDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVL
NNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVL
CYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHA
MKFNHICEQTRFKN*

Vector Type

Bacterial

Antibiotic

Amp; Kan

Comments

Silent change at R382 (AGA > AGG) compared to NCBI ref

100042 R 1000 1001 1047 19007

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