MINDY4

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Synonyms
FAM188B
Plasmid
pcDNA FRT TO MINDY4 BirA HA
Parent Plasmid
pcDNA FRT TO c-BirA HA
Expressed
MINDY4_BirA_HA
DU Number
DU59100
Genbank
Q4G0A6-1
Sequence of Insert
AGATCTATGGACAGCCTCTTCGTGGAGGAGGTGGCCGCCTCCTTGGTCAGGGAGTTCCTCAGCAGAAAGGGCTTAAAGAAGACATGTGTGACCATGGACCAGGAACGCCCACGCTCTGACCTCAGCATAAACAACAGAAATGATCTTCGAAAGGTTTTGCATCTTGAATTTCTCTATAAGGAGAACAAGGCAAAGGAAAATCCTCTAAAAACAAGCCTTGAACTCATCACCAGATACTTTCTGGATCACTTTGGAAATACGGCTAACAATTTCACTCAAGATACCCCAATCCCTGCACTCTCAGTTCCAAAGAAAAATAACAAAGTGCCATCAAGATGCTCAGAGACTACACTGGTAAATATATATGACCTTTCAGATGAAGATGCAGGATGGAGAACATCATTGTCAGAAACAAGCAAAGCCAGACATGACAATCTTGATGGAGATGTACTTGGTAATTTTGTATCATCTAAAAGGCCCCCGCACAAAAGTAAGCCCATGCAGACGGTCCCGGGTGAAACTCCTGTGTTGACTTCTGCATGGGAGAAGATAGACAAGCTTCACTCGGAGCCTTCCTTGGATGTGAAGAGGATGGGAGAGAATTCCAGGCCAAAGTCTGGTCTGATTGTGCGAGGCATGATGTCTGGGCCCATCGCCAGCTCCCCACAGGATTCTTTTCACAGACACTATCTGAGACGGTCCTCACCGTCAAGCAGCTCCACCCAACCCCAAGAAGAGAGCCGGAAGGTCCCTGAGCTCTTTGTCTGCACCCAACAGGACATTCTGGCTTCGAGCAACAGCTCCCCCTCCAGGACCTCCCTGGGTCAGCTTAGTGAACTGACCGTAGAAAGGCAGAAAACCACTGCCAGCAGCCCTCCCCATCTGCCCAGCAAACGGCTGCCCCCATGGGACAGGGCCAGGCCGAGGGATCCCTCCGAGGACACCCCAGCAGTGGACGGCAGCACAGACACGGACAGGATGCCCTTGAAGCTCTACTTGCCTGGTGGTAATTCCAGGATGACCCAGGAGAGGCTGGAAAGAGCGTTCAAACGGCAGGGCAGCCAGCCCGCACCTGTCAGGAAAAATCAGTTGCTGCCGTCTGACAAGGTGGATGGTGAGCTGGGTGCCCTGCGGCTCGAGGATGTGGAGGATGAGTTGATAAGGGAAGAGGTCATCCTGTCGCCAGTCCCATCAGTGCTCAAGTTGCAGACAGCATCAAAACCAATTGACCTCTCAGTAGCAAAGGAAATAAAGACCCTTCTGTTTGGTTCCAGCTTTTGCTGTTTCAATGAAGAATGGAAACTTCAGAGTTTTTCCTTTAGTAACACAGCCTCATTAAAATACGGCATAGTGCAGAACAAGGGTGGTCCTTGCGGAGTCCTGGCAGCTGTCCAAGGCTGTGTCCTACAGAAACTCCTGTTTGAAGGAGATAGCAAAGCCGACTGTGCTCAGGGACTGCAGCCTTCAGATGCCCACCGGACCCGCTGCCTCGTCCTGGCCCTCGCAGACATTGTGTGGCGGGCAGGGGGCCGAGAGAGAGCCGTTGTTGCACTGGCTTCGAGAACACAGCAGTTCAGTCCAACAGGGAAATACAAAGCAGATGGAGTCTTAGAAACACTTACGCTTCACAGTTTGACCTGCTATGAGGACCTGGTGACTTTTCTTCAACAAAGCATTCATCAGTTTGAAGTGGGCCCCTATGGCTGCATCCTGCTCACCCTTTCTGCCATCCTGTCCAGGTCTACAGAGCTCATCCGCCAGGACTTTGATGTCCCCACCAGCCACCTGATTGGAGCACATGGCTACTGTACACAGGAACTTGTCAATCTGCTCCTGACTGGGAAAGCTGTGTCCAACGTTTTCAACGATGTGGTTGAGCTGGATTCTGGGGATGGGAACATCACACTTCTCAGAGGCATTGCTGCACGCAGTGATATTGGCTTCTTATCTCTCTTTGAGCATTACAACATGTGCCAGGTTGGCTGCTTCCTGAAGACCCCGAGGTTCCCCATCTGGGTGGTTTGCAGTGAGAGCCACTTCAGCATCCTCTTTAGCCTGCAGCCGGGGCTCCTGCGTGACTGGAGGACTGAGAGGCTCTTTGACTTGTACTACTACGATGGCCTGGCCAACCAGCAGGAGCAGATCCGGCTGACCATTGACACCACCCAAACCATCTCTGAGGACACAGACAACGACCTTGTCCCACCCCTCGAGCTCTGCATCAGAACCAAGTGGAAGGGGGCATCAGTGAACTGGAACGGCTCAGACCCCATCCTGGCGGCCGC
Amino Acid Sequence
MDSLFVEEVAASLVREFLSRKGLKKTCVTMDQERPRSDLSINNRNDLRKVLHLEFLYKENKAKENPLKTSLELITRYFLDHFGNTANNFTQDTPIPALSVPKKNNKVPSRCSETTLVNIYDLSDEDAGWRTSLSETSKARHDNLDGDVLGNFVSSKRPPHKSKPMQTVPGETPVLTSAWEKIDKLHSEPSLDVKRMGENSRPKSGLIVRGMMSGPIASSPQDSFHRHYLRRSSPSSSSTQPQEESRKVPELFVCTQQDILASSNSSPSRTSLGQLSELTVERQKTTASSPPHLPSKRLPPWDRARPRDPSEDTPAVDGSTDTDRMPLKLYLPGGNSRMTQERLERAFKRQGSQPAPVRKNQLLPSDKVDGELGALRLEDVEDELIREEVILSPVPSVLKLQTASKPIDLSVAKEIKTLLFGSSFCCFNEEWKLQSFSFSNTASLKYGIVQNKGGPCGVLAAVQGCVLQKLLFEGDSKADCAQGLQPSDAHRTRCLVLALADIVWRAGGRERAVVALASRTQQFSPTGKYKADGVLETLTLHSLTCYEDLVTFLQQSIHQFEVGPYGCILLTLSAILSRSTELIRQDFDVPTSHLIGAHGYCTQELVNLLLTGKAVSNVFNDVVELDSGDGNITLLRGIAARSDIGFLSLFEHYNMCQVGCFLKTPRFPIWVVCSESHFSILFSLQPGLLRDWRTERLFDLYYYDGLANQQEQIRLTIDTTQTISEDTDNDLVPPLELCIRTKWKGASVNWNGSDPILAAAMKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Kindly clone MINDY4 with BirA R118G_HA fusion in pCDNA5 FRT/TO (MINDY4_BirA R118G_HA). Kindly keep the HA tag at the C-terminus along with BirA and let the N-terminus of the protein be free.
100042 R 1000 1001 1047 19007
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