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Plasmid
pET6His-SUMO-NMNAT2 (C10 Thr to Ala)
Parent Plasmid
pET6His-SUMO
Expressed
6His-SUMO-NMNAT2 (C10 Thr to Ala)
DU Number
DU29095
Tag
6His
SUMO
Species
Human
Sequence of Insert
View Sequence
CTCGAGGCCGCCATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCACCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
CTGCGGCAGCTTCAATCCCATCACCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVEAAMTETTKTHVILLACGSFNPITKGHIQMFERARDYLHK
TGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWEC
YQDTWQTTCSVLEHHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPI
YQNSNVAAKPAAAKILGKVGESLSRICCVRPPVERFAFVDENANLGAVMR
YEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADA
DRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDY
LSQPVIDYILKSQLYINASGAAA*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVEAAMTETTKTHVILLACGSFNPITKGHIQMFERARDYLHK
TGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWEC
YQDTWQTTCSVLEHHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPI
YQNSNVAAKPAAAKILGKVGESLSRICCVRPPVERFAFVDENANLGAVMR
YEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADA
DRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDY
LSQPVIDYILKSQLYINASGAAA*
Vector Type
Bacterial
Antibiotic
Amp
Comments
C-terminal 10 Thr residues mutated to Ala. Parental 45322. Linker still to be deleted
100042 R 1000 1001 1047 19007
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