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Plasmid
pET6His-SUMO-NMNAT2 (N9 Thr to Ala)
Parent Plasmid
pET6His-SUMO
Expressed
6His-SUMO-NMNAT2 (N9 Thr to Ala)
DU Number
DU29094
Tag
6His
SUMO
Species
Human
Sequence of Insert
View Sequence
CTCGAGGCCGCCATGGCCGAGGCCGCCAAGGCCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCAT
CCTCTCCAATGTCAACACACCTTCCATGACACCTGTGATCGGACAGCCAC
AAAACGAGACCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCACCAAG
CCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATG
CCAATCTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACACAGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCAT
CCTCTCCAATGTCAACACACCTTCCATGACACCTGTGATCGGACAGCCAC
AAAACGAGACCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCACCAAG
CCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATG
CCAATCTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACACAGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVEAAMAEAAKAHVILLACGSFNPIAKGHIQMFERARDYLHK
AGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWEC
YQDAWQAACSVLEHHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPI
YQNSNVATKPTAAKILGKVGESLSRICCVRPPVERFTFVDENANLGTVMR
YEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADT
DRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLALQHGDGHVVDY
LSQPVIDYILKSQLYINASGAAA*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVEAAMAEAAKAHVILLACGSFNPIAKGHIQMFERARDYLHK
AGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWEC
YQDAWQAACSVLEHHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPI
YQNSNVATKPTAAKILGKVGESLSRICCVRPPVERFTFVDENANLGTVMR
YEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADT
DRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLALQHGDGHVVDY
LSQPVIDYILKSQLYINASGAAA*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N-terminal 9 Thr residues mutated to Ala. 45322 parental. linker still to be deleted
100042 R 1000 1001 1047 19007
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