NMNAT2 | Ubiquitylation Resource

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Plasmid

pcDNA5D FRT/TO- NMNAT2 ThrLess-C-FLAG

Parent Plasmid

pcDNA5D FRT/TO-C-FLAG

Expressed

NMNAT2-FLAG all Thr to Ala

DU Number

DU29084

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

CTCGAGGCCGCCATGGCCGAGGCCGCCAAGGCCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC

Amino Acid Sequence

View Sequence

MAEAAKAHVILLACGSFNPIAKGHIQMFERARDYLHKAGRFIVIGGIVSP
VHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDAWQAACSVLE
HHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPIYQNSNVAAKPAAA
KILGKVGESLSRICCVRPPVERFAFVDENANLGAVMRYEEIELRILLLCG
SDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADADRIMNHSSILRKY
KNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDYLSQPVIDYILKSQ
LYINASGAAADYKDDDDK*

Vector Type

Mammalian

Antibiotic

Amp

Comments

all 19 Thr residues mutated to Ala. Parental 41573

100042 R 1000 1001 1047 19007

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