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Plasmid
pcDNA5D FRT/TO- NMNAT2 N-ThrLess-C-FLAG
Parent Plasmid
pcDNA5D FRT/TO-C-FLAG
Expressed
NMNAT2-FLAG Thr to Ala (9 N-terminal)
DU Number
DU29082
Tag
6His
Species
Human
Sequence of Insert
View Sequence
CTCGAGGCCGCCATGGCCGAGGCCGCCAAGGCCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCAT
CCTCTCCAATGTCAACACACCTTCCATGACACCTGTGATCGGACAGCCAC
AAAACGAGACCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCACCAAG
CCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATG
CCAATCTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACACAGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCAT
CCTCTCCAATGTCAACACACCTTCCATGACACCTGTGATCGGACAGCCAC
AAAACGAGACCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCACCAAG
CCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATG
CCAATCTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACACAGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC
Amino Acid Sequence
View Sequence
MAEAAKAHVILLACGSFNPIAKGHIQMFERARDYLHKAGRFIVIGGIVSP
VHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDAWQAACSVLE
HHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPIYQNSNVATKPTAA
KILGKVGESLSRICCVRPPVERFTFVDENANLGTVMRYEEIELRILLLCG
SDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADTDRIMNHSSILRKY
KNNIMVVKDDINHPMSVVSSTKSRLALQHGDGHVVDYLSQPVIDYILKSQ
LYINASGAAADFKDDDDK*
VHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDAWQAACSVLE
HHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPIYQNSNVATKPTAA
KILGKVGESLSRICCVRPPVERFTFVDENANLGTVMRYEEIELRILLLCG
SDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADTDRIMNHSSILRKY
KNNIMVVKDDINHPMSVVSSTKSRLALQHGDGHVVDYLSQPVIDYILKSQ
LYINASGAAADFKDDDDK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N-terminal 9 Thr residues mutated to Ala. Parental 41573
100042 R 1000 1001 1047 19007
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