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Plasmid
pET15 6HIS 3C UBC13 E55R + replacement of D94/K94 with LPPKGA S96R
Parent Plasmid
pET15 6His 3C
Expressed
6His 3C UBC13 E55R + replacement of D93/K94 with LPPKGA S96R
DU Number
DU27544
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCGCCGGGCTGCCCCGCAGGATCATCAAGGAAACCCAGCGTTTGCTGGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCCGTTATTTTCATGTGGTCATTGCTGGCCCTCAGGATTCCCCCTTTGAGGGAGGGACTTTTAAACTTAGACTATTCCTTCCAGAAGAATACCCAATGGCAGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACAAGTTGGGAAGAATATGTTTAGATATTTTGAAATTACCTCCAAAGGGTGCTTGGCGCCCAGCACTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAGTGCTCCCAATCCAGATGATCCATTAGCAAATGATGTAGCGGAGCAGTGGAAGACCAACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCCATGAATAATATTTAAGAATTC
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLEVLFQGPGSAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLRLFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKLPPKGAWRPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI*
Vector Type
Bacterial
Antibiotic
Amp
Comments
S100R mutant is actually S96R of full length clone. S100R numbering comes from the insertion of extra amino acids as stated above
100042 R 1000 1001 1047 19007
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