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Plasmid
pFBDual GST-Cullin2, 6His-Rbx1
Parent Plasmid
pFastbacDual 6P1
Expressed
6His-Rbx1
DU Number
DU21546
Genbank
NM_014248.2
Tag
GST
Species
Human
Sequence of Insert
View Sequence
gctagcATGCATCATCACCATCACCATATGGCGGCAGCGATGGATGTGGA
TACCCCGAGCGGCACCAACAGCGGCGCGGGCAAGAAGCGCTTTGAAGTGA
AAAAGTGGAATGCAGTAGCCCTCTGGGCCTGGGATATTGTGGTTGATAAC
TGTGCCATCTGCAGGAACCACATTATGGATCTTTGCATAGAATGTCAAGC
TAACCAGGCGTCCGCTACTTCAGAAGAGTGTACTGTCGCATGGGGAGTCT
GTAACCATGCTTTTCACTTCCACTGCATCTCTCGCTGGCTCAAAACACGA
CAGGTGTGTCCATTGGACAACAGAGAGTGGGAATTCCAAAAGTATGGGCA
CTAGggtacc
TACCCCGAGCGGCACCAACAGCGGCGCGGGCAAGAAGCGCTTTGAAGTGA
AAAAGTGGAATGCAGTAGCCCTCTGGGCCTGGGATATTGTGGTTGATAAC
TGTGCCATCTGCAGGAACCACATTATGGATCTTTGCATAGAATGTCAAGC
TAACCAGGCGTCCGCTACTTCAGAAGAGTGTACTGTCGCATGGGGAGTCT
GTAACCATGCTTTTCACTTCCACTGCATCTCTCGCTGGCTCAAAACACGA
CAGGTGTGTCCATTGGACAACAGAGAGTGGGAATTCCAAAAGTATGGGCA
CTAGggtacc
Amino Acid Sequence
View Sequence
MHHHHHHMAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCA
ICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQV
CPLDNREWEFQKYGH*
ICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQV
CPLDNREWEFQKYGH*
Vector Type
Baculovirus
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
(Cullin2 was subcloned BamHI-Not1 from SC20032 into the PPH driven cassette of SC20978 (pFBDual-GST6P-1 derived) thereby replacing the Cullin3 coding region). 6HIS-Rbx1 was previously subcloned Nhe1-Kpn1into the PP10 driven cassette.