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Plasmid
pFBDMb 6His TEVG mouse USP1 delta E222-Q419 and L607-V735 C90A/mouse WDR48
Parent Plasmid
pFBDMb
Expressed
6His TEVG mouse USP1 delta E222-Q419 and L607-V735 C90A
DU Number
DU58269
Genbank
NM_001301414.1
Tag
6His
Species
Mouse
Sequence of Insert
View Sequence
GGATCCGAAACCATGCATCACCATCACCATCACGAAAACCTGTATTTTCAGGGCATGCCTGGCGTCATACCTAGTGAAAGTAATGGGCTTTCAAGAGGCAGTCCATCCAAGAAAAACAGACTGTCTCTGAAGTTTTTTCAGAAAAAGGAAACTAAAAGAGCCTTAGACTTTACTGATTCTCAAGAAAATGAAGAAAAAACTTCTGAATATAGAGGATCTGAGATTGACCAAGTTGTTCCTGCTGCACAGTCCTCCCCAGTAAGCTGCGAGAAGAGAGAGAACTTGTTACCATTTGTGGGATTGAATAACCTTGGCAACACTGCCTATCTGAATAGTATTCTTCAGGTGTTATACTTTTGTCCTGGCTTTAAGACTGGAGTGAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTGAAGGATGACTCTAATCAGAAGGATAAGGGAAGCTGCAAAGAAGAGTCTTTGGCAAGTTATGAGCTTATATGTAGTTTACAGTCCTTAATAATTTCAGTTGAACAGCTTCAGGCTAGTTTTCTCTTAAATCCAGAGAAATATACAGATGAACTTGCTACACAGCCAAGGCGACTGCTGAACACACTCAGGGAACTCAACCCTATGTATGAAGGATTTCTACAGCATGATGCACAGGAAGTGCTACAGTGTATTCTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAAGAAATTGGCTTTGAGCTAGTAGAGAAACTGTTTCAAGGTCAGCTCGTGTTAAGGACTCGGTGTTTGGAATGTGAAAGCTTAACAGAAAGAAGAGAAGATTTCCAGGATATTAGTGTTCCAGTGCAAGAAGATGAGCTTTCCAAGGTCGAGGAGAGCTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCTTGAGATGGGCAATTTCACAGTTTGCTTCAGTGGAGAGAATTGTAGGAGAGGATAAATACTTCTGTGAAAATTGCCATCATTATACGGAAGCAGAGCGAAGTCTCCTGTTTGATAAAATGCCTGAAGTTATCACTATTCATCTGAAGTGCTTTGCTGCTAGTGGCTTGGAGTTTGACTGTTACGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAACTGTCACTAGAAGAATGGAGCACAAAGCCGACGAATGACAGCTATGGATTATTTGCCGTTGTGATGCACAGTGGCATTACTATTAGTAGTGGGCACTATACCGCATCTGTTAAAGTCACTGACCTTAACAGTCTAGAGGTGCAAAGCTTAAAGGAGTACGAGGGGAAGTGGTTGCTCTTTGACGATTCTGAGGTAAAAGTGACAGAAGAGAAAGACTTTCTGAATTCTCTCTCCCCTTCTACATCCCCTACGTCTACTCCCTACTTGCTATTCTATAAAAAATTATAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHENLYFQGMPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKTSEYRGSEIDQVVPAAQSSPVSCEKRENLLPFVGLNNL
GNTAYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLR
ELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTL
RWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHS
GITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*
GNTAYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLR
ELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTL
RWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHS
GITISSGHYTASVKVTDLNSLEVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Mutagenesis of DU58112
100042 R 1000 1001 1047 19007
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