IRAK2 | Ubiquitylation Resource

Plasmid

pFastbacDual 6HIS TEV GST 3C AVI IRAK2 192-507

Parent Plasmid

pFastbac

Expressed

6HIS TEV GST 3C AVI IRAK2 192-507

DU Number

DU56367

Tag

GST

6His

AVI

Species

Human

Sequence of Insert

View Sequence

GGCCTGAACGATATTTTTGAAGCGCAGAAAATTGAATGGCATGAAGGCAGCGGCGGCAGCGGCCTGGGATCCGCTGGAGACAGCCTTTTCTGGAGTGAGG
CAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATT
CGTCTTCAAGAAGCTCAGAGAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATTTGTCTTAGATGCTGCCACCCC
AATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAGTTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGTC
AGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAG
CAACGTCAAGAGCTCTAATGTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTCAAAATAC
ACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCGTATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGCT
GTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGCCCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAG
CAGCACCGCCTCGCTCTGCTCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAGGCTTCCG
GAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTGTGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAAG
AGCGGCTCCGAGGTCGGGAGTAGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGCTCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAA
TTCCAGCCTTGATGCCTCCTCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAATGGGGAAGGAAGGCTGCGGGTC
ATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGTGTTGGCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAGG
CCAAAAGGAAACTGATGGAGAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATTGAGCTCTTTGGCCCCTGAgcggccgc

Amino Acid Sequence

View Sequence

MHHHHHHENLYFQGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCP
KERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAI
PQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPGLNDIFEAQKIEWHEGSGGSGLGSAGDSLFWSEADVVQATDDFNQNRKISQGTFADVY
RGHRHGKPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEY
LHGLEIIHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLK
DLLLSDIPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRE*

Vector Type

Baculovirus

Antibiotic

Amp

Comments

Please insert Avitag sequence (GLNDIFEAQKIEWHE) between 3C cleavage site and protein with 6 aa spacer (e.g. GSGGSG). DU51963 might be suitable template.

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