MYCBP2 | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-Myc-MYCBP2 Leu3348-Phe4640

Parent Plasmid

pcDNA5-FRT/TO-Myc

Expressed

Myc-MYCBP2 Leu3348-Phe4640

DU Number

DU28845

Tag

Myc

Species

Human

Sequence of Insert

View Sequence

ggatccCTTCCTGTGAAAATGCCTTGTCTCTACCTGCAGACATTAGCTAG
GCATCATCATGAAAATTTTGTGGGCTATCAAGATGACAATCTATTCCAGG
ATGAAATGAGATATCTACGTTCAACATCTGTACCTGCCCCGTATATATCA
GTAACTCCTGATGCAAGTCCTAATGTATTTGAAGAGCCAGAGAGCAATAT
GAAGTCTATGCCACCAAGTTTAGAAACCAGTCCCATAACTGATACTGATC
TTGCAAAGAGAACTGTCTTCCAAAGATCATACTCAGTTGTTGCTTCCGAA
TATGATAAACAACACTCCATTTTACCTGCACGAGTTAAAGCTATTCCTAG
AAGAAGAGTTAACAGTGGAGACACTGAAGTTGGTTCTTCCCTTTTGAGAC
ATCCGTCTCCTGAGCTTTCTCGGCTAATCTCAGCCCACAGCTCTCTTTCT
AAAGGAGAACGAAATTTCCAGTGGCCAGTTTTAGCTTTTGTTATACAACA
TCATGATCTAGAAGGTCTTGAAATAGCAATGAAACAGGCCCTAAGGAAAT
CTGCTTGTCGAGTTTTTGCTATGGAGGCTTTCAACTGGCTTCTGTGTAAT
GTCATCCAAACCACTTCTCTCCATGATATTCTGTGGCATTTTGTGGCATC
ACTGACTCCTGCACCAGTGGAACCAGAGGAAGAAGAGGATGAAGAAAATA
AAACAAGCAAAGAAAATTCAGAACAAGAGAAAGATACAAGAGTATGTGAA
CATCCACTCTCAGACATAGTGATTGCCGGGGAAGCTGCTCATCCTTTACC
ACACACCTTTCACCGCTTGCTGCAGACCATCTCAGACCTTATGATGTCTC
TCCCCAGCGGCAGTTCATTACAGCAAATGGCCCTGAGGTGCTGGAGTCTC
AAATTCAAGCAATCTGATCACCAGTTCCTTCATCAGAGCAACGTCTTTCA
TCACATTAACAATATTTTGTCAAAGTCAGATGATGGCGATAGTGAAGAGA
GTTTTAGCATCAGTATACAGTCTGGCTTTGAAGCTATGAGTCAGGAATTA
TGCATAGTAATGTGCTTAAAGGACTTAACCAGCATTGTTGACATAAAAAC
TTCAAGCCGACCTGCCATGATTGGCAGTTTGACAGACGGCTCCACAGAAA
CCTTTTGGGAATCAGGAGATGAAGATAAAAACAAAACTAAGAACATCACC
ATCAACTGTGTAAAAGGAATCAATGCCCGCTATGTGTCTGTTCACGTGGA
CAATTCCCGAGATCTTGGGAATAAAGTTACCTCAATGACCTTCTTAACTG
GCAAAGCAGTAGAAGATTTGTGCAGAATAAAGCAGGTTGATCTGGATTCC
AGGCACATTGGCTGGGTAACAAGTGAACTTCCAGGAGGGGATAATCACAT
CATAAAAATTGAATTAAAAGGCCCAGAAAATACACTGAGAGTTCGACAAG
TCAAAGTCCTGGGCTGGAAAGATGGTGAAAGCACAAAAATAGCTGGCCAG
ATTTCAGCCAGTGTGGCCCAGCAGAGGAACTGTGAAGCTGAGACTCTGCG
AGTATTCAGACTGATTACGTCTCAAGTATTTGGAAAGCTCATCTCTGGAG
ATGCTGAACCTACACCAGAACAAGAGGAAAAAGCACTATTGTCATCACCT
GAAGGAGAAGAAAAAGTATACAATGCAACATCAGATGCTGACCTGAAAGA
ACATATGGTTGGAATCATATTCAGCAGGAGTAAGCTGACTAACTTACAAA
AACAGGTGTGTGCTCATATTGTCCAAGCTATTCGCATGGAAGCTACCAGA
GTCCGTGAAGAATGGGAACATGCTATATCAAGCAAAGAAAATGCCAATTC
TCAGCCAAATGATGAAGATGCCTCCTCTGATGCCTACTGCTTTGAGCTGC
TCTCTATGGTTTTAGCACTGAGTGGCTCTAACGTTGGCCGGCAATATCTG
GCTCAACAGCTAACCCTGCTTCAGGATCTCTTCTCGCTGCTTCACACAGC
CTCTCCTAGAGTCCAGAGACAGGTAACCTCTTTACTAAGAAGAGTTTTGC
CTGAAGTAACCCCTAGTCGTCTGGCCAGCATCATAGGAGTGAAATCCCTC
CCCCCAGCAGATATCAGTGATATCATTCACTCAACAGAGAAAGGAGACTG
GAATAAGCTGGGTATCTTGGACATGTTTCTAGGATGCATTGCCAAAGCAC
TCACTGTACAGCTAAAAGCCAAAGGAACCACCATCACTGGAACAGCTGGT
ACCACTGTGGGCAAAGGAGTTACAACAGTTACTCTTCCGATGATTTTCAA
TTCCAGTTATCTCCGACGAGGTGAAAGTCATTGGTGGATGAAGGGCTCAA
CCCCTACCCAGATCTCAGAGATCATCATTAAACTTATCAAGGATATGGCA
GCAGGTCATCTGTCAGAAGCTTGGTCCCGAGTGACAAAAAATGCTATTGC
AGAAACCATCATTGCCTTGACCAAGATGGAAGAAGAATTTAGGTCTCCAG
TGAGATGTATTGCAACAACTAGACTCTGGCTTGCTCTCGCATCCCTATGT
GTTCTTGATCAGGACCACGTAGATCGTCTCTCCTCGGGGAGATGGATGGG
AAAGGATGGACAACAAAAACAAATGCCTATGTGTGATAACCATGATGATG
GTGAAACTGCAGCAATCATTTTATGCAATGTCTGTGGAAATTTATGTACA
GACTGTGACAGATTCCTTCACCTTCATCGAAGAACCAAAACTCATCAAAG
ACAGGTCTTCAAAGAAGAAGAAGAAGCTATAAAGGTTGACCTTCATGAAG
GTTGTGGTAGAACCAAATTGTTCTGGTTGATGGCACTGGCAGATTCTAAA
ACAATGAAGGCAATGGTGGAATTCCGAGAACACACAGGCAAACCCACCAC
GAGTAGCTCAGAAGCATGTCGCTTCTGTGGTTCCAGGAGTGGAACAGAGT
TATCTGCTGTTGGCAGTGTTTGTTCTGATGCAGATTGCCAGGAATACGCT
AAGATAGCCTGTAGTAAGACGCATCCTTGTGGCCATCCATGCGGGGGTGT
TAAAAACGAAGAGCACTGTCTGCCCTGTCTACACGGCTGTGACAAAAGTG
CCACAAGCCTGAAGCAAGACGCCGATGACATGTGCATGATATGTTTCACC
GAAGCGCTCTCGGCAGCACCAGCCATTCAGCTGGATTGTAGTCACATATT
CCACTTACAGTGCTGTCGGCGAGTATTAGAAAATCGATGGCTTGGCCCAA
GGATAACATTTGGATTTATATCTTGTCCCATTTGCAAGAACAAAATTAAT
CACATAGTACTAAAAGACCTACTTGATCCAATAAAAGAACTCTATGAGGA
TGTCAGAAGAAAAGCCTTAATGAGATTGGAATATGAAGGTCTGCATAAGA
GTGAAGCTATCACAACTCCTGGTGTGAGGTTTTATAATGACCCAGCTGGC
TATGCAATGAATAGATATGCATATTATGTGTGCTACAAATGCAGAAAGGC
ATATTTTGGTGGTGAAGCTCGCTGCGATGCTGAGGCTGGACGGGGAGATG
ATTATGATCCCAGAGAGCTCATTTGTGGTGCCTGTTCTGATGTTTCCAGG
GCTCAGATGTGTCCCAAACATGGCACAGACTTTTTGGAATATAAATGTCG
CTACTGCTGTTCAGTGGCTGTTTTTTTCTGTTTTGGAACAACACATTTTT
GTAATGCTTGTCATGATGATTTTCAAAGAATGACTAGCATTCCTAAGGAA
GAACTACCACACTGTCCTGCAGGTCCCAAAGGCAAGCAGTTAGAAGGAAC
TGAATGTCCACTCCATGTTGTTCATCCACCCACTGGGGAAGAGTTTGCTC
TGGGATGTGGAGTGTGCAGAAATGCCCACACTTTTTAGgcggccgc

Amino Acid Sequence

View Sequence

MEQKLISEEDLGSLPVKMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYL
RSTSVPAPYISVTPDASPNVFEEPESNMKSMPPSLETSPITDTDLAKRTV
FQRSYSVVASEYDKQHSILPARVKAIPRRRVNSGDTEVGSSLLRHPSPEL
SRLISAHSSLSKGERNFQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVF
AMEAFNWLLCNVIQTTSLHDILWHFVASLTPAPVEPEEEEDEENKTSKEN
SEQEKDTRVCEHPLSDIVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSS
LQQMALRCWSLKFKQSDHQFLHQSNVFHHINNILSKSDDGDSEESFSISI
QSGFEAMSQELCIVMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESG
DEDKNKTKNITINCVKGINARYVSVHVDNSRDLGNKVTSMTFLTGKAVED
LCRIKQVDLDSRHIGWVTSELPGGDNHIIKIELKGPENTLRVRQVKVLGW
KDGESTKIAGQISASVAQQRNCEAETLRVFRLITSQVFGKLISGDAEPTP
EQEEKALLSSPEGEEKVYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAH
IVQAIRMEATRVREEWEHAISSKENANSQPNDEDASSDAYCFELLSMVLA
LSGSNVGRQYLAQQLTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPS
RLASIIGVKSLPPADISDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLK
AKGTTITGTAGTTVGKGVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQIS
EIIIKLIKDMAAGHLSEAWSRVTKNAIAETIIALTKMEEEFRSPVRCIAT
TRLWLALASLCVLDQDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAI
ILCNVCGNLCTDCDRFLHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTK
LFWLMALADSKTMKAMVEFREHTGKPTTSSSEACRFCGSRSGTELSAVGS
VCSDADCQEYAKIACSKTHPCGHPCGGVKNEEHCLPCLHGCDKSATSLKQ
DADDMCMICFTEALSAAPAIQLDCSHIFHLQCCRRVLENRWLGPRITFGF
ISCPICKNKINHIVLKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITT
PGVRFYNDPAGYAMNRYAYYVCYKCRKAYFGGEARCDAEAGRGDDYDPRE
LICGACSDVSRAQMCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCNACHD
DFQRMTSIPKEELPHCPAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVC
RNAHTF*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Parental 44556

100042 R 1000 1001 1047 19007

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