This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
UBP
Plasmid
pGEX6P1 human USP1 M1-V60 rat USP2 M271-M618(end) C289A
Parent Plasmid
pGEX6P1
Expressed
GST 3C human USP1 M1-V60 rat USP2 M271-M618(end) C289A
DU Number
DU28829
Genbank
BC085719.1 (USP2) NM_001017415.1 (USP1)
Tag
GST
Sequence of Insert
View Sequence
GGATCCATGCCTGGTGTCATACCTAGTGAAAGTAATGGACTTTCAAGAGG
TAGCCCTTCAAAGAAAAACAGACTTTCCTTAAAGTTTTTTCAGAAAAAGG
AAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGAAAAA
GCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGGATCCATGAATTC
AAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGGCCT
TCATGAACTCAATTCTTCAGTGTCTGAGCAACACCCGCGAGTTGAGAGAT
TACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCATACCAGCAGTGC
ACACACAGCTCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGA
CGTCATCCCCCAATGATGTGGTGAGCCCATCTGAGTTCAAGACCCAGATC
CAGAGATATGCACCACGCTTCGTTGGCTATAATCAGCAGGATGCTCAGGA
ATTCCTCCGTTTCCTTCTGGATGGCCTCCACAACGAGGTGAACCGGGTGG
CAGCAAGGCCTAAGCCCAGCCCTGAGAGCCTTGATCATCTCCCTGATGAA
GAGAAAGGGCGACAGATGTGGAGGAAGTATCTAGAAAGGGAAGACAGTCG
GATTGGGGATCTCTTCGTTGGGCAGCTAAAGAGCTCCCTGACGTGCACCG
ACTGCGGCTACTGCTCTACAGTGTTCGATCCCTTCTGGGACCTCTCGTTG
CCCATTGCAAAGAGAGGCTATCCTGAAGTGACGTTAATGGATTGTATGAG
GCTCTTCACCAAAGAGGATGTGTTGGATGGGGATGAGAAACCAACATGCT
GCCGCTGCCGGGCCAGAAAACGATGTATAAAGAAGTTCTCTGTCCAGAGG
TTCCCAAAGATCTTGGTGCTCCACCTGAAGCGGTTCTCAGAATCCAGGAT
ACGAACCAGCAAGCTCACAACATTTGTGAATTTCCCACTAAGAGACCTGG
ACTTGAGAGAATTTGCTTCAGAAAACACCAACCATGCTGTTTACAACCTG
TATGCTGTGTCCAATCACTCCGGAACCACCATGGGAGGTCACTATACAGC
CTACTGCCGAAGTCCGGTTACGGGCGAATGGCACACTTTCAATGACTCCA
GTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTATTTG
CTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATGTAGGCGGCCGC
TAGCCCTTCAAAGAAAAACAGACTTTCCTTAAAGTTTTTTCAGAAAAAGG
AAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGAAAAA
GCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGGATCCATGAATTC
AAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGGCCT
TCATGAACTCAATTCTTCAGTGTCTGAGCAACACCCGCGAGTTGAGAGAT
TACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCATACCAGCAGTGC
ACACACAGCTCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGA
CGTCATCCCCCAATGATGTGGTGAGCCCATCTGAGTTCAAGACCCAGATC
CAGAGATATGCACCACGCTTCGTTGGCTATAATCAGCAGGATGCTCAGGA
ATTCCTCCGTTTCCTTCTGGATGGCCTCCACAACGAGGTGAACCGGGTGG
CAGCAAGGCCTAAGCCCAGCCCTGAGAGCCTTGATCATCTCCCTGATGAA
GAGAAAGGGCGACAGATGTGGAGGAAGTATCTAGAAAGGGAAGACAGTCG
GATTGGGGATCTCTTCGTTGGGCAGCTAAAGAGCTCCCTGACGTGCACCG
ACTGCGGCTACTGCTCTACAGTGTTCGATCCCTTCTGGGACCTCTCGTTG
CCCATTGCAAAGAGAGGCTATCCTGAAGTGACGTTAATGGATTGTATGAG
GCTCTTCACCAAAGAGGATGTGTTGGATGGGGATGAGAAACCAACATGCT
GCCGCTGCCGGGCCAGAAAACGATGTATAAAGAAGTTCTCTGTCCAGAGG
TTCCCAAAGATCTTGGTGCTCCACCTGAAGCGGTTCTCAGAATCCAGGAT
ACGAACCAGCAAGCTCACAACATTTGTGAATTTCCCACTAAGAGACCTGG
ACTTGAGAGAATTTGCTTCAGAAAACACCAACCATGCTGTTTACAACCTG
TATGCTGTGTCCAATCACTCCGGAACCACCATGGGAGGTCACTATACAGC
CTACTGCCGAAGTCCGGTTACGGGCGAATGGCACACTTTCAATGACTCCA
GTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTATTTG
CTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATGTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPGVIPSESNGLSRGSPSK
KNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVGSMNSKSAQ
GLAGLRNLGNTAFMNSILQCLSNTRELRDYCLQRLYMRDLGHTSSAHTAL
MEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRF
LLDGLHNEVNRVAARPKPSPESLDHLPDEEKGRQMWRKYLEREDSRIGDL
FVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTK
EDVLDGDEKPTCCRCRARKRCIKKFSVQRFPKILVLHLKRFSESRIRTSK
LTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRS
PVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPGVIPSESNGLSRGSPSK
KNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVGSMNSKSAQ
GLAGLRNLGNTAFMNSILQCLSNTRELRDYCLQRLYMRDLGHTSSAHTAL
MEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRF
LLDGLHNEVNRVAARPKPSPESLDHLPDEEKGRQMWRKYLEREDSRIGDL
FVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTK
EDVLDGDEKPTCCRCRARKRCIKKFSVQRFPKILVLHLKRFSESRIRTSK
LTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRS
PVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM*
Vector Type
Bacterial
Antibiotic
Amp
Comments
C289A rUSP2
100042 R 1000 1001 1047 19007
Download datasheet