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Plasmid
pcDNA5D FRT/TO Flag RNF216 P826A P827A codon opt
Parent Plasmid
pcDNA5D FRT/TO FLAG
Expressed
FLAG RNF216 P826A P827A
DU Number
DU27055
Genbank
NM_207111.3
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGAAGAGGGCAACAACAACGAGGAAGTGATCCACCTGAACAACTTCCACTGCCACCGGGGCCAAGAGTGGATCAACCTGAGAGATGGCCCCATCACCATCAGCGACAGCAGCGACGAAGAAAGAATCCCCATGCTGGTCACCCCTGCTCCTCAGCAGCACGAGGAAGAAGATCTGGACGACGACGTGATCCTGACCGAGACAAACAAGCCCCAGCGGAGCAGACCCAACCTGATTAAGCCTGCTGCTCAGTGGCAGGACCTGAAGAGACTGGGCGAAGAGAGGCCCAAGAAGTCCAGAGCCGCCTTCGAGAGCGACAAGAGCAGCTACTTCAGCGTGTGCAACAACCCTCTGTTCGACTCTGGCGCCCAGGACGACAGCGAGGATGATTACGGCGAGTTCCTGGATCTGGGCCCTCCTGGCATCAGCGAGTTTACAAAGCCTAGCGGCCAGACCGAGAGAGAGCCTAAGCCTGGACCTAGCCATAACCAGGCCGCCAACGACATCGTGAACCCCAGATCCGAGCAGAAAGTGATCATCCTGGAAGAAGGCAGCCTGCTGTATACCGAGAGCGATCCCCTGGAAACCCAGAACCAGAGCAGCGAGGACAGCGAGACAGAGCTGCTGAGCAATCTGGGAGAGTCTGCCGCTCTGGCCGATGACCAGGCCATTGAGGAAGATTGCTGGCTGGATCACCCTTACTTCCAGAGCCTGAACCAGCAGCCTCGCGAGATCACCAATCAGGTGGTGCCCCAAGAGAGACAGCCTGAGGCTGAACTGGGCAGACTGCTGTTCCAGCACGAGTTCCCCGGACCTGCCTTTCCTAGACCTGAACCTCAGCAAGGCGGCATCTCTGGCCCATCTTCTCCCCAACCTGCTCACCCTCTGGGCGAGTTTGAAGATCAGCAGCTGGCCTCCGACGACGAGGAACCTGGACCTGCATTCCCCATGCAAGAGAGCCAAGAGCCAAACCTGGAAAACATCTGGGGACAAGAGGCCGCCGAGGTGGACCAAGAACTGGTGGAACTGCTGGTCAAAGAGACAGAGGCCAGATTTCCCGACGTGGCCAACGGCTTCATCGAGGAAATCATCCACTTCAAGAACTACTACGACCTGAACGTGCTGTGCAACTTCCTGCTGGAAAACCCTGACTACCCTAAGAGAGAGGACCGGATCATCATCAACCCCAGCTCTAGCCTGCTGGCCTCTCAGGACGAGACTAAGCTGCCCAAGATCGACTTCTTCGACTACAGCAAGCTGACCCCTCTGGACCAGCGGTGCTTTATCCAGGCAGCCGATCTGCTGATGGCCGACTTCAAGGTGCTGTCCAGCCAGGACATTAAGTGGGCCCTGCACGAGCTGAAGGGCCACTACGCCATTACCAGAAAGGCCCTGAGCGACGCCATCAAGAAGTGGCAAGAGCTGAGCCCTGAGACAAGCGGCAAGCGGAAGAAACGGAAGCAGATGAACCAGTACAGCTACATCGATTTCAAGTTCGAGCAGGGCGACATCAAGATCGAGAAGCGGATGTTCTTTTTGGAGAACAAGCGGCGGCACTGCAGAAGCTACGACAGAAGGGCTCTGCTGCCTGCCGTGCAGCAAGAGCAAGAGTTCTATGAGCAGAAAATCAAAGAAATGGCCGAACACGAGGACTTTTTGCTGGCCCTGCAGATGAATGAGGAACAGTACCAGAAGGACGGCCAGCTGATCGAGTGCAGATGTTGCTACGGGGAGTTCCCATTCGAGGAACTGACCCAGTGTGCCGACGCTCACCTGTTCTGCAAAGAGTGCCTGATCAGATACGCCCAAGAGGCTGTGTTCGGCTCCGGCAAGCTGGAACTGAGCTGTATGGAAGGCAGCTGCACCTGTAGCTTCCCCACCAGCGAGCTGGAAAAGGTGCTGCCTCAGACCATCCTGTACAAGTACTACGAGCGGAAGGCCGAGGAAGAGGTGGCCGCTGCTTATGCCGATGAGCTTGTGCGGTGTCCCTCCTGCTCTTTCCCTGCACTGCTGGATAGCGACGTGAAGAGATTCAGCTGCCCCAATCCTCACTGCCGGAAAGAAACCTGCCGGAAGTGTCAAGGCCTGTGGAAAGAACACAACGGCCTGACCTGCGAAGAACTGGCCGAGAAGGACGATATCAAGTACCGGACCAGCATCGAAGAGAAGATGACCGCCGCCAGAATCCGGAAGTGCCACAAGTGTGGAACCGGCCTGATCAAGAGCGAGGGCTGCAACAGAATGAGCTGCAGATGCGGAGCCCAGATGTGCTACCTGTGCCGGGTGTCCATCAACGGCTACGACCACTTCTGTCAGCACCCTAGAAGTCCCGGCGCTCCCTGTCAAGAGTGCAGCAGATGTAGCCTGTGGACAGACCCCACCGAGGACGACGAGAAGCTGATTGAGGAAATCCAGAAAGAGGCCGAAGAGGAACAGAAGCGCAAGAACGGCGAGAACACCTTCAAGCGGATCGGCGCTGCACTGGAAAAGCCCGTGGAAAAAGTGCAGAGAGTGGAAGCCCTGCCTCGGCCTGTTCCACAGAATCTGCCTCAGCCACAGATGCCTCCATACGCCTTTGCTCACCCACCATTTCCGCTGCCTCCAGTGCGGCCCGTGTTCAACAATTTCCCTCTGAACATGGGCCCCATTCCAGCTCCATACGTGCCACCTCTGCCTAACGTGCGCGTGAACTACGACTTCGGCCCCATCCACATGCCTCTGGAACACAACCTGCCTATGCACTTCGGACCCCAGCCTCGGCACAGATTCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGAALEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Codon optimised sequence
100042 R 1000 1001 1047 19007
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