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Plasmid
pcDNA5D FRT/TO-HA-OTUB1-BirA R118G
Parent Plasmid
pcDNA5D FRT/TO-HA-MCS-BirA R118G
Expressed
HA-OTUB1-BirA R118G
DU Number
DU28357
Genbank
NM_017670
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCGGCGGAGGAACCTCAGCAGCAGAAGCAGGAGCCGCTGGG
CAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGG
CTCAGCAGGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTG
TCAGAGCGGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGA
CAACATCTATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACA
TCCGCAAGACCAGGCCTGACGGCAACTGTTTCTATCGGGCTTTCGGATTC
TCCCACTTGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCAA
GGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTG
AATTCACAATTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAG
GTGGAGAAGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCA
GAGCACCTCCGACTACCTTGTGGTCTACCTGCGGCTGCTCACCTCGGGCT
ACCTGCAGCGCGAGAGCAAGTTCTTCGAGCACTTCATCGAGGGTGGACGG
ACTGTCAAGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGAG
CGACCACATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCC
AGGTGGAGTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATC
TTCCCTGAGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACA
CTACGATATCCTCTACAAAGCGGCCGC
CAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGG
CTCAGCAGGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTG
TCAGAGCGGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGA
CAACATCTATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACA
TCCGCAAGACCAGGCCTGACGGCAACTGTTTCTATCGGGCTTTCGGATTC
TCCCACTTGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCAA
GGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTG
AATTCACAATTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAG
GTGGAGAAGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCA
GAGCACCTCCGACTACCTTGTGGTCTACCTGCGGCTGCTCACCTCGGGCT
ACCTGCAGCGCGAGAGCAAGTTCTTCGAGCACTTCATCGAGGGTGGACGG
ACTGTCAAGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGAG
CGACCACATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCC
AGGTGGAGTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATC
TTCCCTGAGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACA
CTACGATATCCTCTACAAAGCGGCCGC
Amino Acid Sequence
View Sequence
MYPYDVPDYAGSMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQ
QEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPD
GNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDF
HNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESK
FFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDR
GEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYKAAAMKDNTVPLKLIALL
ANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPI
QLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQA
GRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRV
EESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSR
WEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEI
SLRSAEK*
QEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPD
GNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDF
HNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESK
FFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDR
GEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYKAAAMKDNTVPLKLIALL
ANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPI
QLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQA
GRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRV
EESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSR
WEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEI
SLRSAEK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental 49114
100042 R 1000 1001 1047 19007
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