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Plasmid
pET15 6His MBP TEV RNF214
Parent Plasmid
pET15 6HIS MBP DU
Expressed
6His MBP TEV RNF214
DU Number
DU53099
Genbank
NM_001077239.1 GI:116235455
Tag
6His
MBP
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCAGCGTCTGAGGTTGCTGGTGTTGTGGCCAATGCCCCCAGTCCTCCGGAATCTTCTAGTTTATGTGCTTCCAAATCAGACGAAGGTCTCCCAGATGGTCTAAGCACCAAAGACTCTGCACAGAAGCAGAAGAACTCGCCTCTGTTGAGTGTAAGTAGCCAAACAATAACCAAGGAGAATAACAGAAATGTCCATTTGGAGCACTCAGAGCAGAATCCTGGTTCATCAGCAGGTGACACCTCAGCAGCGCACCAGGTGGTTTTAGGAGAAAACTTGATAGCCACAGCCCTTTGTCTTTCTGGCAGTGGGTCTCAGTCTGATTTGAAGGATGTGGCCAGCACAGCAGGAGAGGAGGGGGACACAAGCCTTCGGGAGAGCCTCCATCCAGTCACTCGGTCTCTTAAGGCAGGGTGCCATACTAAGCAGCTTGCCTCCAGGAATTGCTCTGAAGAGAAATCCCCACAAACCTCCATCCTAAAGGAAGGTAACAGGGACACAAGCTTGGATTTCCGACCTGTAGTGTCTCCAGCAAATGGGGTTGAAGGAGTCCGAGTGGATCAGGATGATGATCAAGATAGCTCTTCCCTGAAGCTTTCTCAGAACATTGCTGTACAGACTGACTTTAAGACAGCTGATTCAGAGGTAAACACAGATCAAGATATTGAAAAGAATTTGGATAAAATGATGACAGAGAGAACCCTGTTGAAAGAGCGTTACCAGGAGGTCCTGGACAAACAGAGGCAAGTGGAGAATCAGCTCCAAGTGCAATTAAAGCAGCTTCAGCAAAGGAGAGAAGAGGAAATGAAGAATCACCAGGAGATATTAAAGGCTATTCAGGATGTGACAATAAAGCGGGAAGAAACAAAGAAGAAGATAGAGAAAGAGAAGAAGGAGTTTTTGCAGAAGGAGCAGGATCTGAAAGCTGAAATTGAGAAGCTTTGTGAGAAGGGCAGAAGAGAGGTGTGGGAAATGGAACTGGATAGACTCAAGAATCAGGATGGCGAAATAAATAGGAACATTATGGAAGAGACTGAACGGGCCTGGAAGGCAGAGATCTTATCACTAGAGAGCCGGAAAGAGTTACTGGTACTGAAACTAGAAGAAGCAGAAAAAGAGGCAGAATTGCACCTTACTTACCTCAAGTCAACTCCCCCAACACTGGAGACAGTTCGTTCCAAACAGGAGTGGGAGACGAGACTGAATGGAGTTCGGATAATGAAAAAGAATGTTCGTGACCAATTTAATAGTCATATCCAGTTAGTGAGGAACGGAGCCAAGCTGAGCAGCCTTCCTCAAATCCCTACTCCCACTTTACCTCCACCCCCATCAGAGACAGACTTCATGCTTCAGGTGTTTCAACCCAGTCCCTCTCTGGCTCCTCGGATGCCCTTCTCCATTGGGCAGGTCACAATGCCCATGGTTATGCCCAGTGCAGATCCCCGCTCCTTGTCTTTCCCAATCCTGAACCCTGCCCTTTCCCAGCCCAGCCAGCCTTCCTCACCCCTTCCTGGCTCCCATGGCAGAAATAGCCCTGGCTTGGGTTCCCTTGTCAGCCCCCACGGTCCACACATGCCCCCTGCCGCCTCCATCCCACCTCCCCCAGGCTTGGGCGGTGTTAAGGCTTCTGCTGAAACTCCCCGGCCCCAACCAGTAGACAAACTGGAGAAGATCCTGGAGAAGCTGCTGACCCGGTTCCCACAGTGCAATAAGGCCCAGATGACCAACATTCTTCAGCAGATCAAGACAGCACGTACCACCATGGCAGGCCTGACCATGGAGGAACTTATCCAGTTGGTTGCTGCACGACTGGCAGAACATGAGCGGGTGGCAGCAAGTACTCAGCCACTTGGTCGCATCCGGGCCTTGTTCCCTGCTCCACTGGCCCAAATCAGTACCCCAATGTTCTTGCCTTCTGCCCAAGTTTCATATCCTGGAAGGTCTTCACATGCTCCAGCCACCTGTAAGCTATGTCTAATGTGCCAGAAACTCGTCCAGCCCAGTGAGCTGCATCCAATGGCGTGTACCCATGTATTGCACAAGGAGTGTATCAAATTCTGGGCCCAGACCAACACAAATGACACTTGTCCCTTTTGTCCAACTCTTAAATGA
Amino Acid Sequence
View Sequence
MGSSHHHHHHGSSMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQ
DKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNA
GAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT
DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLY
FQGHMGSMAASEVAGVVANAPSPPESSSLCASKSDEGLPDGLSTKDSAQKQKNSPLLSVSSQTITKENNRNVHLEHSEQNPGSSAGDTSAAHQVVLGENL
IATALCLSGSGSQSDLKDVASTAGEEGDTSLRESLHPVTRSLKAGCHTKQLASRNCSEEKSPQTSILKEGNRDTSLDFRPVVSPANGVEGVRVDQDDDQD
SSSLKLSQNIAVQTDFKTADSEVNTDQDIEKNLDKMMTERTLLKERYQEVLDKQRQVENQLQVQLKQLQQRREEEMKNHQEILKAIQDVTIKREETKKKI
EKEKKEFLQKEQDLKAEIEKLCEKGRREVWEMELDRLKNQDGEINRNIMEETERAWKAEILSLESRKELLVLKLEEAEKEAELHLTYLKSTPPTLETVRS
KQEWETRLNGVRIMKKNVRDQFNSHIQLVRNGAKLSSLPQIPTPTLPPPPSETDFMLQVFQPSPSLAPRMPFSIGQVTMPMVMPSADPRSLSFPILNPAL
SQPSQPSSPLPGSHGRNSPGLGSLVSPHGPHMPPAASIPPPPGLGGVKASAETPRPQPVDKLEKILEKLLTRFPQCNKAQMTNILQQIKTARTTMAGLTM
EELIQLVAARLAEHERVAASTQPLGRIRALFPAPLAQISTPMFLPSAQVSYPGRSSHAPATCKLCLMCQKLVQPSELHPMACTHVLHKECIKFWAQTNTN
DTCPFCPTLK*
DKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNA
GAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT
DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLY
FQGHMGSMAASEVAGVVANAPSPPESSSLCASKSDEGLPDGLSTKDSAQKQKNSPLLSVSSQTITKENNRNVHLEHSEQNPGSSAGDTSAAHQVVLGENL
IATALCLSGSGSQSDLKDVASTAGEEGDTSLRESLHPVTRSLKAGCHTKQLASRNCSEEKSPQTSILKEGNRDTSLDFRPVVSPANGVEGVRVDQDDDQD
SSSLKLSQNIAVQTDFKTADSEVNTDQDIEKNLDKMMTERTLLKERYQEVLDKQRQVENQLQVQLKQLQQRREEEMKNHQEILKAIQDVTIKREETKKKI
EKEKKEFLQKEQDLKAEIEKLCEKGRREVWEMELDRLKNQDGEINRNIMEETERAWKAEILSLESRKELLVLKLEEAEKEAELHLTYLKSTPPTLETVRS
KQEWETRLNGVRIMKKNVRDQFNSHIQLVRNGAKLSSLPQIPTPTLPPPPSETDFMLQVFQPSPSLAPRMPFSIGQVTMPMVMPSADPRSLSFPILNPAL
SQPSQPSSPLPGSHGRNSPGLGSLVSPHGPHMPPAASIPPPPGLGGVKASAETPRPQPVDKLEKILEKLLTRFPQCNKAQMTNILQQIKTARTTMAGLTM
EELIQLVAARLAEHERVAASTQPLGRIRALFPAPLAQISTPMFLPSAQVSYPGRSSHAPATCKLCLMCQKLVQPSELHPMACTHVLHKECIKFWAQTNTN
DTCPFCPTLK*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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