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Plasmid
pGEX 3C HALO Thr-6His-4Gly-UbcH7 C17S C137S
Parent Plasmid
pGEX 3C HALO Thr
Expressed
GST-C3-Halo-Thr-His-4Gly-UbcH7 C17S C137S
DU Number
DU28226
Genbank
P68036
Tag
GST
6His
HALO
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGA
AGTTCTGGGTGGCGGGGGTATGGCGGCCAGCAGGAGGCTGATGAAGGAGC
TTGAAGAAATCCGCAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTT
GATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCC
TCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGT
ACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCA
AACATCGACGAAAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAA
CTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCAC
TGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAA
GAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAGGAGTTTAC
AAAGAAATATGGGGAAAAGCGACCTGTGGACTAAGCGGCCGC
AGTTCTGGGTGGCGGGGGTATGGCGGCCAGCAGGAGGCTGATGAAGGAGC
TTGAAGAAATCCGCAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTT
GATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCC
TCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGT
ACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCA
AACATCGACGAAAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAA
CTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCAC
TGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAA
GAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAGGAGTTTAC
AAAGAAATATGGGGAAAAGCGACCTGTGGACTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMGSSHHHHHHSSGL
EVLGGGGMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDN
PPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAE
NWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEF
TKKYGEKRPVD*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMGSSHHHHHHSSGL
EVLGGGGMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDN
PPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAE
NWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEF
TKKYGEKRPVD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
24192 Parental
100042 R 1000 1001 1047 19007
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