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Plasmid
pGEX6P-1-YOD Ala132-Val348 C160A
Parent Plasmid
pGEX 6P-1
Expressed
GST-YOD Ala132-Val348 C160A
DU Number
DU28144
Genbank
NM_018566.3
Tag
GST
Species
Human
Sequence of Insert
View Sequence
ggatccGCATTTACTAAACGTGGTGCTTCTAGTTACGTCAGGGAAACTTTGCCTGTGCTTACCAGAACCGTGGTCCCAGCAGACAACTCTgcCCTCTTTA
CTAGTGTGTACTATGTCGTCGAAGGAGGAGTCTTGAATCCAGCTTGTGCCCCTGAGATGAGACGCCTCATAGCACAAATTGTAGCAAGCGATCCAGACTT
CTATAGTGAGGCAATACTGGGAAAAACAAATCAAGAGTACTGTGACTGGATCAAAAGGGATGACACTTGGGGAGGAGCAATAGAGATATCGATTTTGTCC
AAGTTTTACCAATGTGAAATATGTGTAGTGGATACACAGACAGTAAGAATTGATCGTTTTGGGGAAGATGCAGGATATACCAAAAGGGTTCTGCTTATTT
ATGATGGCATCCACTATGATCCACTTCAGCGTAACTTCCCTGATCCAGATACACCTCCTCTGACCATTTTCTCCTCTAATGATGATATTGTTCTTGTACA
AGCACTGGAATTAGCAGATGAAGCTAGAAGAAGGAGACAGTTTACTGATGTCAACCGCTTCACCCTGAGATGCATGGTATGTCAGAAAGGATTAACTGGA
CAAGCAGAAGCAAGGGAACATGCCAAGGAGACAGGCCATACCAACTTTGGAGAAGTGTGATCGGCCGAAGGGCGAATTCCACATTGGTCGCTGCAGCCCG
GGGGATCC
CTAGTGTGTACTATGTCGTCGAAGGAGGAGTCTTGAATCCAGCTTGTGCCCCTGAGATGAGACGCCTCATAGCACAAATTGTAGCAAGCGATCCAGACTT
CTATAGTGAGGCAATACTGGGAAAAACAAATCAAGAGTACTGTGACTGGATCAAAAGGGATGACACTTGGGGAGGAGCAATAGAGATATCGATTTTGTCC
AAGTTTTACCAATGTGAAATATGTGTAGTGGATACACAGACAGTAAGAATTGATCGTTTTGGGGAAGATGCAGGATATACCAAAAGGGTTCTGCTTATTT
ATGATGGCATCCACTATGATCCACTTCAGCGTAACTTCCCTGATCCAGATACACCTCCTCTGACCATTTTCTCCTCTAATGATGATATTGTTCTTGTACA
AGCACTGGAATTAGCAGATGAAGCTAGAAGAAGGAGACAGTTTACTGATGTCAACCGCTTCACCCTGAGATGCATGGTATGTCAGAAAGGATTAACTGGA
CAAGCAGAAGCAAGGGAACATGCCAAGGAGACAGGCCATACCAACTTTGGAGAAGTGTGATCGGCCGAAGGGCGAATTCCACATTGGTCGCTGCAGCCCG
GGGGATCC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAFTKRGASSYVRETLPVLTRTVVPADNSALFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEA
ILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVLVQALEL
ADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGEV*
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAFTKRGASSYVRETLPVLTRTVVPADNSALFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEA
ILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVLVQALEL
ADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGEV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
OTU+ZnF domains. faulty 3' Not1 site on source clone - can be excised using pSc-B derived 3' BamHI
100042 R 1000 1001 1047 19007
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