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Plasmid
pGEX C3 HALO thrombin-N4BP1 Glu396-Asp896
Parent Plasmid
pGEX C3 HALO thrombin
Expressed
GST HALO N4BP1 Glu396-Asp896
DU Number
DU25000
Genbank
NM_153029.3
Tag
HALO
GST
Species
Human
Sequence of Insert
View Sequence
ggatccgaattttcagctggtacagtgtatccagagaccaacaaaaccaa
aaataaaggtgtttatagcagcacaaatgagcttacaactgattccactc
caaagaaaacacaggctcacacacagcaaaatatggtagaaaaattttct
cagttaccattcaaagtggaagctaaaccatgtacctcaaattgtagaat
taatactttcagaacagtgccaatagaacagaaacatgaagtctggggtt
caaaccagaactacatttgtaacacagaccctgaaactgatggcctttca
ccttctgttgcctctccaagtcccaaagaagtcaattttgtttcaagggg
agcttcaagtcaccagcccagagttccactttttcctgaaaatggtttac
accagcagccagaacccttgcttccaaataatatgaaatctgcctgtgaa
aaacgtttaggatgttgtagttctcctcattctaagccaaattgctcaac
cctttctccaccaatgccactgccccagctgttaccttcggttactgatg
caaggtcggcaggaccttctgatcatattgattcctcagttactggggtt
caaaggtttcgagatactctaaaaataccctacaagctggaattaaaaaa
tgaaccagggagaacggatttgaaacacattgttatagatgggagcaatg
ttgcaattacccatggtctgaaaaagttcttttcttgtcgtggaattgca
attgcagttgaatatttttggaagcttggcaacagaaacatcactgtatt
tgtccctcagtggagaacaaggcgtgatcctaatgtcacagaacagcact
tcttaacccagctccaggagctcggaatattatctttaactcctgcccgg
atggtctttggagaaagaattgcttctcatgatgacaggtttctactaca
cttagcggacaaaactggtggcataattgtgacaaatgataatttcagag
aatttgtgaatgagtcagtctcttggagagaaattattacaaaaaggctg
ctgcagtacacgttcgtgggggacatatttatggttcccgatgatcctct
gggaagaagtggacctcgattagaggagtttcttcagaaggaagtctgtc
ttagagatatgcagcccctactcagtgccctgccaaatgtgggcatgttt
gaccccagcttcagagtccctggcacccaggcagccagcaccagccacca
gcctccgacccggattcagggagccccttcaagccactggctccctcagc
agccccacttcccactgctgccagccctccccagtctccagcagaacctg
cccatgccagctcagagatcttctgcagaaaccaacgagctgagggaagc
ccttctgaagatcttccctgactcagagcaaagactgaaaatagaccaga
tcttggtggcccacccatacatgaaagatctaaatgcgctttctgccatg
gtgttggattgagcggccgc
aaataaaggtgtttatagcagcacaaatgagcttacaactgattccactc
caaagaaaacacaggctcacacacagcaaaatatggtagaaaaattttct
cagttaccattcaaagtggaagctaaaccatgtacctcaaattgtagaat
taatactttcagaacagtgccaatagaacagaaacatgaagtctggggtt
caaaccagaactacatttgtaacacagaccctgaaactgatggcctttca
ccttctgttgcctctccaagtcccaaagaagtcaattttgtttcaagggg
agcttcaagtcaccagcccagagttccactttttcctgaaaatggtttac
accagcagccagaacccttgcttccaaataatatgaaatctgcctgtgaa
aaacgtttaggatgttgtagttctcctcattctaagccaaattgctcaac
cctttctccaccaatgccactgccccagctgttaccttcggttactgatg
caaggtcggcaggaccttctgatcatattgattcctcagttactggggtt
caaaggtttcgagatactctaaaaataccctacaagctggaattaaaaaa
tgaaccagggagaacggatttgaaacacattgttatagatgggagcaatg
ttgcaattacccatggtctgaaaaagttcttttcttgtcgtggaattgca
attgcagttgaatatttttggaagcttggcaacagaaacatcactgtatt
tgtccctcagtggagaacaaggcgtgatcctaatgtcacagaacagcact
tcttaacccagctccaggagctcggaatattatctttaactcctgcccgg
atggtctttggagaaagaattgcttctcatgatgacaggtttctactaca
cttagcggacaaaactggtggcataattgtgacaaatgataatttcagag
aatttgtgaatgagtcagtctcttggagagaaattattacaaaaaggctg
ctgcagtacacgttcgtgggggacatatttatggttcccgatgatcctct
gggaagaagtggacctcgattagaggagtttcttcagaaggaagtctgtc
ttagagatatgcagcccctactcagtgccctgccaaatgtgggcatgttt
gaccccagcttcagagtccctggcacccaggcagccagcaccagccacca
gcctccgacccggattcagggagccccttcaagccactggctccctcagc
agccccacttcccactgctgccagccctccccagtctccagcagaacctg
cccatgccagctcagagatcttctgcagaaaccaacgagctgagggaagc
ccttctgaagatcttccctgactcagagcaaagactgaaaatagaccaga
tcttggtggcccacccatacatgaaagatctaaatgcgctttctgccatg
gtgttggattgagcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSEFSAGTVYPETNKT
KNKGVYSSTNELTTDSTPKKTQAHTQQNMVEKFSQLPFKVEAKPCTSNCR
INTFRTVPIEQKHEVWGSNQNYICNTDPETDGLSPSVASPSPKEVNFVSR
GASSHQPRVPLFPENGLHQQPEPLLPNNMKSACEKRLGCCSSPHSKPNCS
TLSPPMPLPQLLPSVTDARSAGPSDHIDSSVTGVQRFRDTLKIPYKLELK
NEPGRTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITV
FVPQWRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLL
HLADKTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDP
LGRSGPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSH
QPPTRIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELRE
ALLKIFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSEFSAGTVYPETNKT
KNKGVYSSTNELTTDSTPKKTQAHTQQNMVEKFSQLPFKVEAKPCTSNCR
INTFRTVPIEQKHEVWGSNQNYICNTDPETDGLSPSVASPSPKEVNFVSR
GASSHQPRVPLFPENGLHQQPEPLLPNNMKSACEKRLGCCSSPHSKPNCS
TLSPPMPLPQLLPSVTDARSAGPSDHIDSSVTGVQRFRDTLKIPYKLELK
NEPGRTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITV
FVPQWRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLL
HLADKTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDP
LGRSGPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSH
QPPTRIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELRE
ALLKIFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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