USP24 | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-GFP USP24 V2308A

Parent Plasmid

pcDNA5-FRT/TO-GFP

Expressed

GFP-USP24 V2308A

DU Number

DU28020

Genbank

Gene 23358 NP_056121.2 NM_015306.2

Tag

GFP

Species

Human

Sequence of Insert

View Sequence

gcggccgctATGACCACGCTGCTGTGCATGGGCTTCTCAGACCCCGCCACCATCCGCAAGGCCCTGCGCCTGGCCAAGAACGACATTAACGAGGCCGTGG
CACTGCTCACCAACGAGCGGCCGGGCCTCGACTACGGCGGCTACGAGCCCATGGACAGCGGCGGTCTTTCCGAGTCTGATGAGAATTGTAGAAGGTTTAT
GGACAGGTGTATGCCTGAAGCATTTAAAAAGCTCCTGACATCAAGTGCTGTTCACAAGTGGGGTACTGAAATTCATGAAGGAATTTACAACATGTTGATG
CTATTAATAGAACTGGTCGCAGAGAGAATAAAACAAGATCCAATTCCCACTGGTCTCCTGGGTGTGCTTACAATGGCTTTCAATCCTGATAATGAATACC
ATTTTAAAAACAGAATGAAAGTGTCTCAAAGGAATTGGGCAGAAGTGTTTGGAGAGGGAAATATGTTTGCTGTTTCACCTGTATCGACTTTCCAAAAGGA
GCCTCATGGATGGGTTGTGGATTTGGTAAATAAGTTTGGAGAATTAGGTGGATTTGCAGCAATCCAAGCCAAGCTCCATTCAGAAGATATAGAACTTGGG
GCTGTCTCAGCACTGATTCAGCCCTTAGGAGTGTGTGCAGAGTACCTCAATTCCTCCGTGGTACAGCCCATGCTAGACCCAGTCATTCTTACTACAATCC
AGGATGTACGGAGTGTAGAAGAGAAAGACCTCAAAGACAAGAGATTGGTTAGCATCCCTGAGCTCTTGTCTGCCGTTAAGTTACTTTGCATGCGCTTCCA
ACCGGATCTGGTGACAATTGTGGATGACCTTCGACTAGATATTCTATTGCGCATGCTGAAATCACCACATTTCAGTGCTAAGATGAATTCTCTCAAAGAA
GTAACCAAACTAATAGAAGATAGCACTTTATCCAAATCTGTGAAGAATGCTATAGATACAGACAGATTATTAGATTGGCTAGTTGAAAACTCAGTTCTGT
CGATTGCACTGGAAGGCAACATAGACCAAGCACAATACTGTGACCGTATAAAGGGAATTATTGAACTCTTGGGTAGTAAATTGTCGTTAGATGAACTCAC
TAAAATTTGGAAGATACAGTCAGGACAATCATCTACTGTGATTGAGAACATTCATACTATTATTGCTGCAGCGGCTGTGAAATTTAATTCAGATCAGCTT
AATCATTTGTTTGTTCTCATTCAGAAGGTTTTAGACGTACTCTGGGAACTGGCTCACCTTCCAACCCTGCCCAGTAGCCTTATTCAGCAGGCCTTGGAGG
AGCACCTGACAATCCTTAGTGATGCATATGCAGTGAAAGAAGCAATCAAGAGGAGCTACATCATCAAGTGCATAGAAGATATTAAGAGGTCATCTCAGCT
TAATAATCCCCAGTTTGTATGGGTGGTACCAGCTTTGCGTCAGCTCCATGAAATTACTCGCTCATTCATAAAACAAACCTATCAAAAGCAAGACAAGAGC
ATTATTCAAGACTTGAAGAAGAATTTTGAAATAGTGAAATTGGTAACGGGAAGTTTGATCGCTTGTCATCGGCTTGCAGCTGCTGTGGCCGGGCCTGGAG
GCTTAAGTGGCTCGACACTAGTGGATGGCCGGTACACTTACCGGGAGTATTTAGAGGCACATCTAAAATTTCTAGCGTTTTTCTTGCAAGAAGCTACTCT
GTATCTGGGCTGGAATCGTGCCAAGGAGATCTGGGAGTGTCTTGTAACTGGCCAGGATGTTTGTGAATTAGATAGAGAGATGTGTTTTGAATGGTTTACA
AAAGGACAGCATGATCTTGAGAGTGATGTTCAGCAGCAGCTCTTCAAGGAGAAAATTCTTAAATTGGAGTCATATGAAATCACTATGAATGGTTTTAACT
TATTTAAAACTTTTTTTGAAAATGTGAATCTTTGTGATCATCGATTGAAAAGACAAGGAGCTCAGTTGTATGTAGAAAAGCTGGAATTGATAGGAATGGA
TTTCATTTGGAAAATAGCCATGGAATCACCTGATGAAGAAATTGCTAATGAAGCTATTCAGCTAATCATAAACTATAGTTACATTAATCTAAATCCTAGA
TTAAAGAAGGATTCAGTATCTTTACATAAGAAATTCATTGCTGATTGCTACACAAGATTAGAAGCAGCCAGTTCAGCACTTGGTGGCCCCACTCTAACAC
ATGCTGTGACCAGAGCAACAAAAATGCTTACAGCAACTGCCATGCCAACTGTAGCAACCTCAGTTCAGTCTCCTTATAGATCTACTAAACTTGTAATAAT
TGAGAGATTGCTGCTTCTGGCAGAGCGCTATGTGATCACTATAGAGGATTTTTACTCTGTTCCACGAACTATTCTACCTCATGGTGCCTCATTTCATGGA
CATCTTTTAACCCTTAATGTTACCTATGAGTCTACCAAAGATACCTTCACTGTCGAGGCTCACAGTAATGAAACCATAGGGAGTGTCCGGTGGAAAATAG
CCAAGCAGTTGTGCTCTCCTGTGGATAATATACAGATATTTACAAATGATAGCCTGCTGACAGTGAATAAAGATCAAAAGCTACTCCACCAACTGGGCTT
TTCTGATGAACAAATCCTTACAGTGAAGACTTCTGGCAGTGGGACCCCATCTGGGAGTTCAGCAGATTCTTCAACCAGCTCCAGCAGCAGCAGCAGTGGG
GTTTTTAGTTCTTCATATGCCATGGAGCAGGAGAAATCCCTCCCTGGTGTAGTGATGGCTCTCGTATGTAACGTATTTGACATGCTTTATCAGCTCGCCA
ATCTGGAAGAGCCAAGGATAACTCTACGAGTACGGAAGCTTCTGCTCTTGATACCCACTGATCCAGCCATTCAGGAAGCCCTTGATCAACTTGATTCTTT
AGGAAGAAAGAAAACATTGCTGTCTGAATCAAGTTCTCAGTCCTCAAAATCTCCATCCCTGTCATCAAAGCAACAGCACCAGCCAAGTGCCAGTTCAATT
TTAGAAAGTCTGTTTCGATCTTTTGCCCCGGGAATGTCTACCTTCAGAGTGCTCTACAACTTAGAAGTTCTAAGCTCCAAACTCATGCCAACAGCTGATG
ATGACATGGCCAGAAGCTGTGCCAAATCCTTCTGTGAAAACTTCCTCAAAGCTGGCGGTTTGAGTTTGGTTGTAAATGTCATGCAGAGAGACTCCATCCC
ATCAGAAGTAGACTATGAAACAAGGCAGGGTGTTTATTCCATCTGTCTACAGCTTGCAAGATTTTTACTTGTCGGACAAACAATGCCCACGTTATTAGAT
GAAGACCTCACCAAAGATGGTATAGAAGCACTTTCTTCCCGCCCATTCCGAAATGTCAGCCGGCAGACAAGCAGACAGATGTCCTTATGTGGTACCCCAG
AAAAGTCATCCTACCGACAGTTGTCCGTGTCTGATAGGTCTTCTATTAGGGTTGAGGAAATCATCCCTGCTGCTCGAGTTGCAATACAAACAATGGAAGT
AAGTGATTTCACTTCTACTGTGGCTTGCTTCATGAGATTGTCATGGGCTGCGGCTGCAGGACGGCTTGATCTTGTTGGGAGTAGCCAGCCAATTAAAGAA
AGTAATTCCCTGTGTCCTGCTGGAATTCGAAACAGACTCAGCAGTTCAGGAAGCAATTGCAGCTCTGGAAGTGAAGGAGAACCAGTAGCCCTGCATGCGG
GAATCTGTGTTCGACAACAGTCTGTATCCACCAAAGACTCGCTGATTGCGGGAGAGGCTTTGTCTCTTCTTGTTACGTGCCTACAGCTTCGGAGCCAGCA
ACTGGCATCTTTCTATAACTTGCCCTGTGTTGCTGATTTCATCATTGATATTCTGCTCGGATCACCAAGTGCTGAGATTCGCCGGGTTGCCTGTGATCAG
CTGTACACTCTTAGTCAGACAGACACATCAGCGCATCCAGATGTGCAGAAGCCAAATCAGTTTCTTCTAGGCGTAATCCTCACGGCTCAGCTGCCTCTCT
GGTCTCCAACTAGTATTATGAGAGGAGTCAATCAGAGACTGTTATCTCAGTGTATGGAGTATTTTGATTTGAGATGCCAGTTATTAGATGATCTGACAAC
TTCAGAAATGGAGCAGTTAAGGATCAGCCCAGCTACGATGCTTGAAGATGAGATTACTTGGCTGGATAACTTTGAACCTAATCGTACAGCTGAATGTGAG
ACCAGTGAAGCGGACAACATCTTACTGGCAGGGCACTTACGCCTCATCAAGACCCTTCTTTCACTCTGTGGGGCAGAAAAGGAAATGCTTGGTTCATCAC
TCATTAAACCATTGTTAGATGACTTCCTTTTCCGAGCTTCTAGAATTATTTTAAATAGTCATTCTCCAGCTGGCAGTGCCGCCATCAGTCAACAGGACTT
TCATCCAAAGTGTAGTACAGCGAATAGCCGATTGGCAGCCTATGAAGTCCTTGTGATGTTGGCTGATAGTTCACCTTCAAATCTTCAAATTATTATAAAA
GAACTGCTTTCTATGCATCACCAGCCTGACCCTGCTCTTACCAAGGAGTTTGATTACCTTCCCCCAGTGGATAGCAGGTCCAGTTCAGGGTTTGTGGGGC
TGAGAAATGGTGGTGCAACTTGTTATATGAATGCAGTCTTCCAGCAGCTGTATATGCAACCTGGGCTCCCTGAGTCATTACTTTCAGTGGATGATGACAC
AGACAATCCAGATGATAGCGTGTTTTACCAAGTGCAGTCTCTCTTTGGACATTTAATGGAAAGCAAGCTGCAGTACTATGTACCTGAGAATTTTTGGAAG
ATTTTCAAGATGTGGAATAAAGAACTTTATGTGAGAGAACAGCAGGATGCATATGAATTCTTTACTAGTCTCATTGATCAGATGGATGAATACCTCAAGA
AAATGGGGAGAGACCAAATTTTTAAGAATACATTTCAGGGCATCTACTCTGATCAGAAGATCTGTAAAGACTGTCCTCACAGATATGAGCGTGAAGAAGC
TTTCATGGCTCTCAATCTAGGAGTGACTTCTTGTCAGAGTTTGGAAATTTCTTTGGACCAATTTGTTAGAGGAGAAGTTCTAGAAGGAAGTAATGCGTAC
TACTGTGAAAAGTGTAAAGAAAAGAGAATAACAGTGAAAAGGACCTGTATTAAATCTTTACCTAGCGTCTTGGTAATTCACCTAATGAGATTTGGGTTTG
ACTGGGAAAGCGGACGCTCCATTAAATATGATGAACAAATAAGGTTTCCCTGGATGCTAAACATGGAGCCTTACACAGTTTCAGGAATGGCTCGCCAAGA
TTCTTCTTCTGAAGTTGGGGAAAATGGGCGAAGTGTGGATCAGGGCGGTGGAGGATCCCCACGAAAAAAGGTTGCCCTCACAGAAAACTATGAACTTGTC
GGTGTCATCGTACACAGTGGGCAGGCACACGCAGGCCACTACTATTCCTTCATTAAGGACAGGCGAGGGTGTGGAAAAGGAAAGTGGTATAAATTTAATG
ACACAGTTATAGAAGAATTTGACCTAAATGACGAGACCCTGGAGTATGAATGCTTTGGAGGAGAATATAGACCAAAAGTTTATGATCAAACAAACCCATA
CACTGATGTGCGCCGAAGATACTGGAATGCCTATATGCTTTTCTACCAAAGGGTGTCTGATCAGAACTCCCCAGTATTACCAAAGAAAAGTCGAGTCAGC
GTTGTACGGCAGGAAGCTGAGGATCTCTCTCTGTCAGCTCCATCTTCACCAGAAATTTCACCTCAGTCATCCCCTCGGCCCCATAGGCCGAACAATGACC
GGCTGTCTATTCTTACCAAGCTGGTTAAAAAAGGCGAGAAGAAAGGACTGTTTGTGGAGAAAATGCCTGCTCGAATATACCAGATGGTGAGAGATGAGAA
CCTCAAGTTTATGAAGAATAGAGATGTATACAGTAGTGATTATTTCAGTTTTGTTTTGTCTTTAGCTTCATTGAATGCTACTAAATTAAAGCATCCATAT
TATCCTTGCATGGCAAAGGTGAGCTTACAGCTTGCTATTCAATTCCTTTTTCAAACTTATCTACGGACAAAGAAGAAACTCAGGGTTGATACTGAAGAAT
GGATTGCTACCATTGAAGCATTGCTTTCAAAAAGTTTTGATGCTTGTCAGTGGTTAGTTGAATATTTTATTAGTTCTGAAGGACGAGAATTGATAAAGAT
TTTCTTACTGGAGTGCAATGTGAGAGAAGTACGAGTTGCTGTGGCCACCATTCTGGAGAAAACCCTAGACAGTGCCTTGTTTTATCAGGATAAGTTAAAA
AGCCTTCATCAGTTACTGGAGGTACTACTTGCTCTGTTGGACAAAGACGTCCCAGAAAATTGTAAAAACTGTGCTCAGTACTTTTTCCTGTTCAACACTT
TTGTACAAAAGCAAGGAATTAGGGCTGGAGATCTTCTTCTGAGGCATTCAGCTCTGCGGCACATGATCAGCTTCCTCCTAGGGGCCAGTCGGCAAAACAA
TCAGATACGTCGATGGAGTTCAGCACAAGCACGAGAATTTGGGAATCTTCACAATACAGTGGCGTTACTTGTTTTGCATTCAGATGTCTCATCCCAAAGG
AATGTTGCTCCTGGCATATTTAAGCAACGACCACCCATTAGCATTGCTCCCTCAAGCCCTCTGTTGCCCCTCCATGAGGAGGTAGAAGCCTTGTTGTTCA
TGTCTGAAGGGAAACCTTACCTGTTAGAGGTAATGTTTGCTTTGCGGGAGCTGACAGGCTCGCTCTTGGCACTCATTGAGATGGTAGTGTACTGCTGTTT
CTGTAATGAGCATTTTTCCTTCACAATGCTGCATTTCATTAAGAACCAACTAGAAACGGCTCCACCTCATGAGTTAAAGAATACGTTCCAACTACTTCAT
GAAATATTGGTTATTGAAGATCCTATACAAGcAGAGCGAGTCAAATTTGTGTTTGAGACAGAAAATGGATTACTAGCTTTGATGCACCACAGTAATCATG
TGGACAGTAGTCGCTGCTACCAGTGTGTCAAATTTCTTGTCACTCTTGCTCAAAAGTGTCCTGCAGCTAAGGAGTACTTCAAGGAGAATTCCCACCACTG
GAGCTGGGCTGTGCAGTGGCTACAGAAGAAGATGTCAGAACATTACTGGACACCACAGAGTAATGTCTCTAATGAAACATCAACTGGAAAAACCTTTCAG
CGAACCATTTCAGCTCAGGACACGTTAGCGTATGCCACAGCTTTGTTGAATGAAAAAGAGCAATCAGGAAGCAGTAATGGGTCGGAGAGTAGTCCTGCCA
ATGAGAACGGAGACAGGCATCTACAGCAGGGTTCAGAATCTCCCATGATGATTGGTGAGTTGAGAAGTGACCTTGATGATGTTGATCCCTAGgcggccgc

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTIDAAAMTTLLCMGFSDPATIRKALRLAKNDINEAVALLTNERPGLDYGGYE
PMDSGGLSESDENCRRFMDRCMPEAFKKLLTSSAVHKWGTEIHEGIYNMLMLLIELVAERIKQDPIPTGLLGVLTMAFNPDNEYHFKNRMKVSQRNWAEV
FGEGNMFAVSPVSTFQKEPHGWVVDLVNKFGELGGFAAIQAKLHSEDIELGAVSALIQPLGVCAEYLNSSVVQPMLDPVILTTIQDVRSVEEKDLKDKRL
VSIPELLSAVKLLCMRFQPDLVTIVDDLRLDILLRMLKSPHFSAKMNSLKEVTKLIEDSTLSKSVKNAIDTDRLLDWLVENSVLSIALEGNIDQAQYCDR
IKGIIELLGSKLSLDELTKIWKIQSGQSSTVIENIHTIIAAAAVKFNSDQLNHLFVLIQKVLDVLWELAHLPTLPSSLIQQALEEHLTILSDAYAVKEAI
KRSYIIKCIEDIKRSSQLNNPQFVWVVPALRQLHEITRSFIKQTYQKQDKSIIQDLKKNFEIVKLVTGSLIACHRLAAAVAGPGGLSGSTLVDGRYTYRE
YLEAHLKFLAFFLQEATLYLGWNRAKEIWECLVTGQDVCELDREMCFEWFTKGQHDLESDVQQQLFKEKILKLESYEITMNGFNLFKTFFENVNLCDHRL
KRQGAQLYVEKLELIGMDFIWKIAMESPDEEIANEAIQLIINYSYINLNPRLKKDSVSLHKKFIADCYTRLEAASSALGGPTLTHAVTRATKMLTATAMP
TVATSVQSPYRSTKLVIIERLLLLAERYVITIEDFYSVPRTILPHGASFHGHLLTLNVTYESTKDTFTVEAHSNETIGSVRWKIAKQLCSPVDNIQIFTN
DSLLTVNKDQKLLHQLGFSDEQILTVKTSGSGTPSGSSADSSTSSSSSSSGVFSSSYAMEQEKSLPGVVMALVCNVFDMLYQLANLEEPRITLRVRKLLL
LIPTDPAIQEALDQLDSLGRKKTLLSESSSQSSKSPSLSSKQQHQPSASSILESLFRSFAPGMSTFRVLYNLEVLSSKLMPTADDDMARSCAKSFCENFL
KAGGLSLVVNVMQRDSIPSEVDYETRQGVYSICLQLARFLLVGQTMPTLLDEDLTKDGIEALSSRPFRNVSRQTSRQMSLCGTPEKSSYRQLSVSDRSSI
RVEEIIPAARVAIQTMEVSDFTSTVACFMRLSWAAAAGRLDLVGSSQPIKESNSLCPAGIRNRLSSSGSNCSSGSEGEPVALHAGICVRQQSVSTKDSLI
AGEALSLLVTCLQLRSQQLASFYNLPCVADFIIDILLGSPSAEIRRVACDQLYTLSQTDTSAHPDVQKPNQFLLGVILTAQLPLWSPTSIMRGVNQRLLS
QCMEYFDLRCQLLDDLTTSEMEQLRISPATMLEDEITWLDNFEPNRTAECETSEADNILLAGHLRLIKTLLSLCGAEKEMLGSSLIKPLLDDFLFRASRI
ILNSHSPAGSAAISQQDFHPKCSTANSRLAAYEVLVMLADSSPSNLQIIIKELLSMHHQPDPALTKEFDYLPPVDSRSSSGFVGLRNGGATCYMNAVFQQ
LYMQPGLPESLLSVDDDTDNPDDSVFYQVQSLFGHLMESKLQYYVPENFWKIFKMWNKELYVREQQDAYEFFTSLIDQMDEYLKKMGRDQIFKNTFQGIY
SDQKICKDCPHRYEREEAFMALNLGVTSCQSLEISLDQFVRGEVLEGSNAYYCEKCKEKRITVKRTCIKSLPSVLVIHLMRFGFDWESGRSIKYDEQIRF
PWMLNMEPYTVSGMARQDSSSEVGENGRSVDQGGGGSPRKKVALTENYELVGVIVHSGQAHAGHYYSFIKDRRGCGKGKWYKFNDTVIEEFDLNDETLEY
ECFGGEYRPKVYDQTNPYTDVRRRYWNAYMLFYQRVSDQNSPVLPKKSRVSVVRQEAEDLSLSAPSSPEISPQSSPRPHRPNNDRLSILTKLVKKGEKKG
LFVEKMPARIYQMVRDENLKFMKNRDVYSSDYFSFVLSLASLNATKLKHPYYPCMAKVSLQLAIQFLFQTYLRTKKKLRVDTEEWIATIEALLSKSFDAC
QWLVEYFISSEGRELIKIFLLECNVREVRVAVATILEKTLDSALFYQDKLKSLHQLLEVLLALLDKDVPENCKNCAQYFFLFNTFVQKQGIRAGDLLLRH
SALRHMISFLLGASRQNNQIRRWSSAQAREFGNLHNTVALLVLHSDVSSQRNVAPGIFKQRPPISIAPSSPLLPLHEEVEALLFMSEGKPYLLEVMFALR
ELTGSLLALIEMVVYCCFCNEHFSFTMLHFIKNQLETAPPHELKNTFQLLHEILVIEDPIQAERVKFVFETENGLLALMHHSNHVDSSRCYQCVKFLVTL
AQKCPAAKEYFKENSHHWSWAVQWLQKKMSEHYWTPQSNVSNETSTGKTFQRTISAQDTLAYATALLNEKEQSGSSNGSESSPANENGDRHLQQGSESPM
MIGELRSDLDDVDP*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Transcript variant 2 - compared to NCBI ref has 8 residues missing at the extreme N-terminus plus 3 internal deletions (aa61-164) (aa519-550) and (aa605-620). V2308A in variant 2 corresponds to V2468A in NCBI ref.
Parental 33042 is also Transcript variant 2

100042 R 1000 1001 1047 19007

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