1. Home
  3. SHARPIN

SHARPIN

This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
SHANK-associated RH domain interactor (SHARPIN), SIPL1
Plasmid
pCDFDuet-1-6His-HOIP UBA-RBR domain/ Sharpin E173-S346
Parent Plasmid
pSC-B
Expressed
Sharpin E173-S346
DU Number
DU24937
Genbank
AAH25244.1 NM_030974.4 → NP_112236.3
Species
Human
Sequence of Insert
CATATGGAAGAGCTGGCAGGGAGCCTGGCCCGGGCTATTGCAGGTGGAGACGAGAAGGGGGCAGCCCAAGTGGCAGCCGTCCTGGCCCAGCATCGTGTGGCCCTGAGTGTTCAGCTTCAGGAGGCCTGCTTCCCACCTGGCCCCATCAGGCTGCAGGTCACACTTGAAGACGCTGCCTCTGCCGCATCCGCCGCGTCCTCTGCACACGTTGCCCTGCAGGTCCACCCCCACTGCACTGTTGCAGCTCTCCAGGAGCAGGTGTTCTCAGAGCTCGGTTTCCCGCCAGCCGTGCAACGCTGGGTCATCGGACGGTGCCTGTGTGTGCCTGAGCGCAGCCTTGCCTCTTACGGGGTTCGGCAGGATGGGGACCCTGCTTTCCTCTACTTGCTGTCAGCTCCCCGAGAAGCCCCAGCCACAGGACCTAGCCCTCAGCACCCCCAGAAGATGGACGGGGAACTTGGACGCTTGTTTCCCCCATCATTGGGGCTACCCCCAGGCCCCCAGCCAGCTGCCTCCAGCCTGCCCAGTTAGCTCGAG
Amino Acid Sequence
MEELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRL
QVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWV
IGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDG
ELGRLFPPSLGLPPGPQPAASSLPS*
Vector Type
Bacterial
Antibiotic
Spectinomycin
Comments
silent change made at Pro303 (CCT > CCC) to knock out native Xho1 site at nt909.
Transcript Variant 1
100042 R 1000 1001 1047 19007
Download datasheet