OTUB1 | Ubiquitylation Resource

Plasmid

pNHD1.3-GST-OTUB S16TAG-6His

Parent Plasmid

pNHD1.3

Expressed

GST-OTUB S16TAG-6His

DU Number

DU24923

Tag

GST

6His

Species

Human

Sequence of Insert

View Sequence

tgtacaATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCA
ACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATT
TGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTG
GGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATT
AACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGT
TGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCG
GTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTT
TGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAA
TGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTA
ACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACAT
GGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAAC
GTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTAT
ATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCA
TCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCA
TGGCGGCGGAGGAACCTCAGCAGCAGAAGCAGGAGCCGCTGGGCTAGGAC
TCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGGCTCAGCA
GGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTGTCAGAGC
GGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGACAACATC
TATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACATCCGCAA
GACCAGGCCTGACGGCAACTGTTTCTATCGGGCTTTCGGATTCTCCCACT
TGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCAAGGCTGTG
TCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTGAATTCAC
AATTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAGGTGGAGA
AGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCAGAGCACC
TCCGACTACCTTGTGGTCTACCTGCGGCTGCTCACCTCGGGCTACCTGCA
GCGCGAGAGCAAGTTCTTCGAGCACTTCATCGAGGGTGGACGGACTGTCA
AGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGAGCGACCAC
ATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCCAGGTGGA
GTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATCTTCCCTG
AGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACACTACGAT
ATCCTCTACAAACACCATCATCATCATCACTAAGGTACC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAEEPQQQKQEPLG*DSE
GVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQ
QKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSA
KSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSD
YLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIH
IIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDIL
YKHHHHHH*

Vector Type

Bacterial

Antibiotic

Tetracycline

Comments

amber stop at S16, parental 47463

100042 R 1000 1001 1047 19007