Ub-TEV-Halo | Ubiquitylation Resource

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Plasmid

pNHD 1.3 Ub20tag HALO 6HIS

Parent Plasmid

pNHD1.3

Expressed

Ub(Ser20stop)-TEV-Halo 6HIS

DU Number

DU51288

Species

Human

Sequence of Insert

View Sequence

CATATGCAGATTTTCGTCAAGACGTTAACCGGTAAGACCATAACTCTAGAAGTGGAACCGTAGGATACCATCGAAAACGTGAAAGCGAAAATCCAGGATA
AAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCAGATTATAACATCCAGAAAGAGAGTAC
CCTGCATCTGGTCTTAAGACTGCGTGGCGGGGAAAACCTGTATTTTCAGGGCATGGCAGAAATCGGTACTGGCTTTCCATTCGACCCCCATTATGTGGAA
GTCCTGGGCGAGCGCATGCACTACGTCGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTCCTACGTGTGGCGCA
ACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGACCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGA
CCACGTCCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCACGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCC
AAGCGCAATCCAGAGCGCGTCAAAGGTATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGCCCGCGAGACCTTCC
AGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCAGAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGA
AGTCGAGATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTTCCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCG
AACATCGTCGCGCTGGTCGAAGAATACATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGTTCTGATCCCACCGG
CCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGTGGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGG
CAGCGAGATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCCATCACCATCACCACCATTAATAAGGTACC

Amino Acid Sequence

View Sequence

MQIFVKTLTGKTITLEVEP*DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGENLYFQGMAEIGTGFPFDPHYVEV
LGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAK
RNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPAN
IVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGHHHHHH*

Vector Type

Bacterial

Antibiotic

Tetracycline

Comments

Plasmid pNHD Ub20TAG, C-ter His tag to be replaced with TEV-Halo. Thanks!
Created using in fusion

100042 R 1000 1001 1047 19007

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