SHARPIN | Ubiquitylation Resource

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Synonyms

SHANK-associated RH domain interactor (SHARPIN), SIPL1

Plasmid

pSc-B-Sharpin E173-D320

Parent Plasmid

pSC-B

DU Number

DU24884

Genbank

AAH25244.1 NM_030974.4 → NP_112236.3

Species

Human

Sequence of Insert

View Sequence

CATATGGAAGAGCTGGCAGGGAGCCTGGCCCGGGCTATTGCAGGTGGAGACGAGAAGGGGGCAGCCCAAGTGGCAGCCGTCCTGGCCCAGCATCGTGTGGCCCTGAGTGTTCAGCTTCAGGAGGCCTGCTTCCCACCTGGCCCCATCAGGCTGCAGGTCACACTTGAAGACGCTGCCTCTGCCGCATCCGCCGCGTCCTCTGCACACGTTGCCCTGCAGGTCCACCCCCACTGCACTGTTGCAGCTCTCCAGGAGCAGGTGTTCTCAGAGCTCGGTTTCCCGCCAGCCGTGCAACGCTGGGTCATCGGACGGTGCCTGTGTGTGCCTGAGCGCAGCCTTGCCTCTTACGGGGTTCGGCAGGATGGGGACCCTGCTTTCCTCTACTTGCTGTCAGCTCCCCGAGAAGCCCCAGCCACAGGACCTAGCCCTCAGCACCCCCAGAAGATGGACGGAAGGGCGAATTC

Amino Acid Sequence

View Sequence

EELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRL
QVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWV
IGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMD

Vector Type

Other

Antibiotic

Amp

Comments

silent change made at Pro303 (CCT > CCC) to knock out native Xho1 site at nt909. 1st round intermediate to producing Sharpin E173-S346 by primer extension
Transcript Variant 1 numbering

100042 R 1000 1001 1047 19007

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