This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Plasmid
pcDNA BirA R118G VCPIP1 C219A HA
Parent Plasmid
pcDNA BirA R118G-MCS-HA
Expressed
BirA R118G-VCPIP1 C219A-HA
DU Number
DU47478
Tag
HA
BirA
Sequence of Insert
View Sequence
AGATCTATGTCTCAGCCGCCGCCGCCGCCGCCTCCGTTGCCGCCGCCACCTCCTCCCCCTGAGGCTCCACAGACTCCGTCGTCCTTGGCGTCGGCGGCTGCTTCGGGGGGGCTTTTGAAGCGGAGAGACCGGAGAATCCTTTCCGGGAGCTGCCCGGATCCGAAGTGTCAGGCGCGTCTATTTTTCCCGGCCTCCGGTTCTGTCAGCATCGAGTGTACCGAGTGCGGCCAGCGGCACGAGCAGCAACAGCTGCTGGGGGTTGAGGAGGTGACCGACCCGGACGTAGTGCTACACAACCTGCTGCGGAACGCGCTGCTCGGGGTTACGGGGGCACCCAAGAAGAACACGGAACTGGTAAAGGTGATGGGCCTTTCCAACTATCACTGCAAATTGTTGTCGCCCATATTAGCTCGCTATGGAATGGACAAACAGACAGGCCGGGCCAAGCTTCTCCGGGACATGAACCAGGGCGAACTGTTCGATTGCGCCTTACTGGGTGACCGCGCCTTCCTCATAGAACCAGAGCATGTTAACACTGTGGGCTATGGCAAGGACCGCTCCGGAAGCCTCCTGTATTTGCATGACACTCTGGAGGACATTAAGCGGGCCAATAAAAGCCAGGAATGTCTCATTCCAGTGCATGTGGACGGGGATGGACACGCCTTGGTGCATGCTGTGTCTCGGGCTCTAGTAGGCCGAGAGCTCTTCTGGCATGCCTTAAGAGAGAATCTTAAACAGCACTTTCAGCAGCACCTGGCCCGATATCAAGCTCTGTTCCATGACTTCATTGATGCTGCTGAGTGGGAGGACATTATCAATGAGTGTGACCCTCTGTTTGTACCACCTGAGGGTGTTCCCTTGGGCCTGAGGAATATCCACATATTTGGTCTTGCCAATGTGCTACATCGTCCTATTATTCTGTTAGATTCCCTCAGTGGCATGAGAAGCTCTGGTGATTATTCAGCCACCTTTCTACCTGGGCTCATCCCTGCAGAGAAGTGCACTGGGAAAGATGGTCATTTGAACAAACCAATCTGTATTGCATGGAGCAGCTCCGGTAGAAACCATTATATCCCCTTGGTAGGCATAAAAGGGGCTGCTTTGCCCAAACTGCCTATGAATTTGCTTCCTAAAGCATGGGGTGTGCCTCAGGACCTTATTAAAAAGTACATAAAACTTGAAGAGGATGGTGGTTGTGTTATTGGAGGTGACAGAAGTTTGCAAGATAAATACTTACTTAGGCTTGTTGCTGCTATGGAAGAAGTCTTTATGGACAAACATGGTATCCATCCTAGTTTGGTTGCTGATGTCCATCAGTATTTCTACAGAAGGACTGGAGTGATAGGAGTTCAGCCTGAGGAAGTCACAGCAGCTGCTAAAAAAGCAGTAATGGATAATCGCCTTCACAAATGTTTGCTCTGTGGTGCCCTTTCTGAACTTCATGTTCCTCCAGAGTGGTTGGCTCCTGGAGGGAAATTGTATAACCTGGCAAAAAGTACTCATGGACAGCTGAGGACTGACAAAAATTACAGCTTTCCCTTGAACAATTTGGTTTGCTCATATGATTCAGTGAAAGATGTTCTGGTACCAGACTATGGAATGAGTAACCTAACAGCTTGTAATTGGTGCCATGGCACATCTGTGCGAAAGGTCAGAGGAGATGGGTCTATTGTGTATTTGGATGGAGACAGAACTAATTCTAGGTCCACTGGTGGCAAATGTGGTTGTGGATTCAAACACTTTTGGGATGGTAAGGAGTATGACAATCTACCAGAAGCTTTCCCTATTACTTTAGAATGGGGTGGAAGAGTGGTCAGAGAAACAGTATATTGGTTCCAGTATGAAAGTGATTCATCTTTGAATAGTAATGTTTACGATGTTGCAATGAAACTTGTTACCAAGCACTTTCCAGGTGAATTTGGGAGTGAAATCCTAGTTCAGAAAGTTGTCCACACTATATTGCATCAGACTGCCAAAAAGAATCCCGATGATTATACTCCTGTAAATATAGATGGTGCTCACGCCCAAAGAGTTGGAGATGTTCAAGGACAAGAATCAGAGTCTCAGCTCCCAACTAAAATTATTCTTACTGGACAGAAAACAAAAACTTTGCACAAGGAGGAGTTAAACATGAGTAAAACTGAAAGAACTATTCAACAGAATATTACGGAACAGGCTTCTGTAATGCAGAAACGGAAAACAGAGAAGTTAAAACAAGAACAAAAAGGGCAACCCAGGACTGTTTCTCCCAGTACCATTCGTGATGGTCCATCCTCTGCACCTGCTACACCTACCAAGGCTCCCTATTCACCGACAACTTCTAAGGAGAAGAAGATCCGAATCACAACTAATGATGGACGACAGTCCATGGTTACCCTTAAGTCTTCAACAACCTTTTTTGAACTTCAGGAAAGTATAGCCAGAGAATTCAACATTCCTCCATATTTACAGTGTATTCGATACGGGTTTCCTCCTAAAGAGTTAATGCCACCACAGGCAGGAATGGAAAAGGAACCAGTTCCTTTACAGCATGGCGACAGAATTACAATAGAAATTCTAAAAAGTAAAGCTGAAGGTGGTCAGTCTGCTGCAGCACACTCAGCCCACACTGTGAAACAAGAAGATATTGCTGTTACTGGTAAACTGTCATCTAAGGAACTTCAGGAGCAAGCTGAAAAAGAAATGTACTCCTTGTGTCTTTTAGCAACATTAATGGGAGAAGATGTATGGTCTTATGCAAAGGGACTTCCTCACATGTTTCAGCAGGGTGGTGTATTCTACAGTATTATGAAGAAAACCATGGGTATGGCTGATGGCAAGCATTGTACTTTTCCACATCTGCCTGGCAAAACCTTTGTCTATAATGCTTCTGAAGATAGACTGGAATTGTGTGTGGATGCTGCAGGACATTTCCCCATTGGTCCTGATGTTGAAGATTTAGTTAAAGAGGCTGTAAGTCAGGTTCGAGCAGAGGCTACTACAAGAAGTAGGGAATCAAGTCCCTCACATGGGCTATTAAAACTAGGTAGTGGTGGAGTAGTGAAAAAGAAATCTGAGCAACTTCATAACGTAACTGCCTTTCAGGGAAAAGGGCATTCTTTAGGAACTGCATCTGGTAACCCACACCTTGATCCAAGAGCTAGGGAAACTTCAGTTGTAAGAAAGCATAATACAGGGACAGACTTTAGTAATAGTTCCACTAAAACAGAGCCTTCTGTATTCACAGCTTCTTCTAGTAATAGTGAGCTTATTCGAATAGCTCCTGGAGTAGTAACAATGAGAGACGGCAGGCAGCTTGATCCTGATTTGGTTGAGGCCCAGCGAAAAAAATTGCAGGAAATGGTTTCTTCTATTCAGGCTTCAATGGACAGGCACCTTCGGGATCAAAGTACAGAGCAGTCACCATCTGATCTTCCTCAAAGGAAAACAGAAGTTGTGAGTTCTTCTGCAAAGTCTGGGAGTCTTCAGACTGGTTTGCCTGAATCTTTTCCTTTAACTGGTGGTACTGAAAATTTGAATACAGAAACAACTGATGGCTGTGTAGCAGATGCACTGGGAGCAGCCTTTGCCACAAGGTCAAAAGCACAAAGGGGAAATTCCGTGGAGGAGCTTGAAGAGATGGATAGTCAAGATGCTGAGATGACTAACACAACTGAGCCAATGGATCACTCTTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKGSMSQPPPPPPPLPPPPPPPEAPQTPSSLASAAASGGLLKRRDRRILSGSCPDPKCQARLFFPASGSVSIECTECGQRHEQQQLLGVEEVTDPDVVLHNLLRNALLGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELFDCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIPVHVDGDGHALVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTGKDGHLNKPICIAWSSSGRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDKYLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAKKAVMDNRLHKCLLCGALSELHVPPEWLAPGGKLYNLAKSTHGQLRTDKNYSFPLNNLVCSYDSVKDVLVPDYGMSNLTACNWCHGTSVRKVRGDGSIVYLDGDRTNSRSTGGKCGCGFKHFWDGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDSSLNSNVYDVAMKLVTKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGAHAQRVGDVQGQESESQLPTKIILTGQKTKTLHKEELNMSKTERTIQQNITEQASVMQKRKTEKLKQEQKGQPRTVSPSTIRDGPSSAPATPTKAPYSPTTSKEKKIRITTNDGRQSMVTLKSSTTFFELQESIAREFNIPPYLQCIRYGFPPKELMPPQAGMEKEPVPLQHGDRITIEILKSKAEGGQSAAAHSAHTVKQEDIAVTGKLSSKELQEQAEKEMYSLCLLATLMGEDVWSYAKGLPHMFQQGGVFYSIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIGPDVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGHSLGTASGNPHLDPRARETSVVRKHNTGTDFSNSSTKTEPSVFTASSSNSELIRIAPGVVTMRDGRQLDPDLVEAQRKKLQEMVSSIQASMDRHLRDQSTEQSPSDLPQRKTEVVSSSAKSGSLQTGLPESFPLTGGTENLNTETTDGCVADALGAAFATRSKAQRGNSVEELEEMDSQDAEMTNTTEPMDHSZAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet