USP15 | Ubiquitylation Resource

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Plasmid

pcDNAfrtto-Flag-USP15 tv1

Parent Plasmid

pcDNA5 FRT/TO FLAG1

Expressed

Flag-USP15 tv1

DU Number

DU24863

Genbank

Gene 9958 isoform 1 = NM_001252078.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

gcggccgcTATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTC
TGACATCGCGACGCTGCTCAAAACCTCGCTCCGGAAAGGGGACACCTGGT
ACCTAGTCGATAGTCGCTGGTTCAAACAGTGGAAAAAATATGTTGGCTTT
GACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTGTATCCTGGACC
CATTGATAACTCTGGACTTCTCAAAGATGGTGATGCCCAGTCACTTAAGG
AACACCTTATTGATGAATTGGATTACATACTGTTGCCAACTGAAGGTTGG
AATAAACTTGTCAGCTGGTACACATTGATGGAAGGTCAAGAGCCAATAGC
ACGAAAGGTGGTTGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAG
TATATCTCACAGAATTGAAGCTATGTGAAAATGGAAACATGAATAATGTT
GTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACAATTGAAAAGGA
AATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGGA
ACAAATACATGAGTAACACATTTGAACCACTGAATAAACCAGACAGCACC
ATTCAGGATGCTGGTTTATACCAAGGACAGGTATTAGTGATAGAACAGAA
AAATGAAGATGGAACATGGCCAAGGGGTCCTTCTACTCCTAAGTCCCCAG
GTGCATCCAATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCA
AATAATTATAACAACATGAACAACAGAAATGTGAAAAACTCAAATTACTG
TCTTCCATCATATACCGCTTATAAGAACTATGATTATTCGGAACCTGGAA
GAAACAATGAACAGCCAGGCCTCTGTGGCCTAAGTAACTTGGGAAATACG
TGTTTCATGAACTCAGCTATTCAGTGTTTGAGCAACACACCTCCACTTAC
TGAGTATTTCCTCAATGATAAGTATCAAGAAGAACTGAATTTTGACAATC
CCTTAGGAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTGATCAAG
CAAATGTGGTCTGGAAAGTTTAGCTACGTCACCCCAAGAGCCTTTAAGAC
ACAGGTAGGACGTTTTGCACCTCAGTTCTCTGGATATCAGCAGCAAGACT
GTCAAGAACTGTTAGCTTTCCTATTAGATGGATTACATGAGGATTTGAAT
AGAATTAGGAAAAAACCATATATACAATTAAAAGATGCAGATGGAAGGCC
AGATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCATTTAAAACGAAATG
ATTCTATCATAGTAGATATATTTCATGGCCTTTTCAAATCAACTTTAGTT
TGTCCTGAGTGTGCTAAGATTTCAGTAACATTTGATCCTTTTTGTTACTT
GACACTTCCATTGCCCATGAAAAAAGAACGCACCTTGGAAGTTTACTTAG
TTAGAATGGATCCACTTACCAAACCTATGCAGTACAAAGTGGTTGTCCCC
AAAATTGGAAACATATTAGATCTTTGTACAGCATTGTCTGCTTTGTCAGG
AATACCTGCAGATAAGATGATAGTTACTGATATATACAATCATAGATTTC
ACAGAATATTCGCTATGGATGAAAACCTTAGTAGTATTATGGAACGGGAT
GATATTTATGTGTTTGAAATTAACATCAATAGGACAGAAGATACAGAGCA
CGTGATTATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCGAGTTATA
CCCACCATACTGGTTCTTCACTTTTTGGTCAGCCCTTTCTTATGGCTGTA
CCACGAAACAATACTGAAGACAAACTTTATAATCTCCTGCTCTTGAGAAT
GTGCCGATATGTCAAAATATCTACTGAAACTGAAGAAACTGAAGGATCCC
TACACTGCTGTAAGGACCAAAATATTAATGGGAATGGCCCAAATGGCATA
CATGAAGAAGGCTCACCAAGTGAAATGGAAACAGATGAGCCAGATGATGA
ATCCAGCCAGGATCAAGAACTTCCCTCAGAGAATGAAAACAGTCAGTCTG
AAGATTCAGTTGGAGGAGATAATGATTCTGAAAATGGATTATGTACTGAG
GATACTTGCAAAGGTCAACTCACGGGACACAAAAAACGATTGTTTACATT
CCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGATGATA
CCAGGCATATAAGATTTGATGATAGGCAGCTTAGGCTAGATGAAAGATCT
TTTCTTGCTTTGGATTGGGATCCTGATTTGAAAAAAAGATATTTTGATGA
AAATGCTGCCGAGGACTTTGAAAAACATGAAAGTGTGGAGTATAAACCTC
CTAAAAAACCCTTTGTGAAATTAAAAGATTGCATTGAACTTTTTACAACA
AAAGAAAAGCTAGGTGCTGAAGATCCCTGGTATTGTCCGAATTGTAAAGA
ACATCAGCAAGCCACAAAGAAATTGGATTTATGGTCCCTGCCTCCAGTAC
TTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGACAAG
TTGGATACCTTAGTTGATTTTCCTATCAATGACTTGGATATGTCGGAATT
CTTAATTAATCCAAATGCAGGTCCTTGCCGCTATAATCTGATTGCTGTTT
CCAACCACTATGGAGGGATGGGAGGAGGACACTATACTGCTTTTGCAAAA
AATAAAGATGATGGAAAATGGTACTATTTTGATGACAGTAGTGTCTCCAC
TGCATCTGAAGACCAAATTGTGTCCAAAGCAGCATATGTACTCTTCTACC
AGAGACAAGACACTTTCAGTGGAACTGGCTTTTTTCCTCTTGACCGAGAA
ACTAAAGGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATGAAGA
TAGCAATGATAATGACAATGATATAGAAAATGAAAACTGTATGCACACTA
ACTAAgcggccgc

Amino Acid Sequence

View Sequence

MDYKDDDDKGSPGEFASLEKLAAAMAEGGAADLDTQRSDIATLLKTSLRK
GDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDA
QSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKH
CKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKE
TRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPST
PKSPGASNFSTLPKISPSSLSNNYNNMNNRNVKNSNYCLPSYTAYKNYDY
SEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEEL
NFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGY
QQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENH
LKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERTL
EVYLVRMDPLTKPMQYKVVVPKIGNILDLCTALSALSGIPADKMIVTDIY
NHRFHRIFAMDENLSSIMERDDIYVFEININRTEDTEHVIIPVCLREKFR
HSSYTHHTGSSLFGQPFLMAVPRNNTEDKLYNLLLLRMCRYVKISTETEE
TEGSLHCCKDQNINGNGPNGIHEEGSPSEMETDEPDDESSQDQELPSENE
NSQSEDSVGGDNDSENGLCTEDTCKGQLTGHKKRLFTFQFNNLGNTDINY
IKDDTRHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESV
EYKPPKKPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWS
LPPVLVVHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFLINPNAGPCRYN
LIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAY
VLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDIENEN
CMHTN*

Vector Type

Mammalian

Antibiotic

Amp

Comments

two rounds of insertion mutagenesis on 42486 with NW3444/45 and then NW3539/40 to build full length tv1. Silent mutation at Ala767 compared to NCBI ref

100042 R 1000 1001 1047 19007

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