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Synonyms
CG13745; Dmel\CG13745; fanci
Plasmid
pBacPAK? 6His-GST 3C Drosophila FancI M1-K1411
Expressed
6His-GST 3C Drosophila FancI M1-K1411
DU Number
DU50185
Genbank
NM_136585.3 GI:665399661 with changes - see notes
Tag
GST
6His
Species
Drosophila Melanogaster
Amino Acid Sequence
View Sequence
MGHHHHHHGSTMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKER
AEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQI
DKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLSLDPCRRPMRSLGEKGRFDELELLIQNTPIEKLKETLKSTMTRSEGITYWNYLLRGFNPE
SKDALAKRFECVRSFMDGLSSTELSYKQTFDLITRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRD
TTVRSIATMQWPASILTPIADMFKTMNLSSGEIVTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLD
SIDLFSDKELREAEETILHHLNYCTMYKLTEKHLAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVW
YRDTLQRKQVDLQQVLTVLIDQNKDGKDVITPGLVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADT
YTVSECMKPIQSVLEHMQWLSGEQSMRMMNFILPLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGY
SIMTQNTLGSRSNPQRNFDMLTLEIIGMLRNCLQQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSS
DDQHMEIQLKDNLGKLIQFVANCLAVFDRAPSGYDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQI
LPLFNQHCKLLESLKALSDASKKAHKQLKQTAKNNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLS
VTASRVESIRLEPDYKQLAYSKRTFKQLTDITKVIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYT
IQDVLDDFMTEYGTDDSICKNEFVSKLPVYLLESLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHV
LGVQNEDAPSESVELTLKSISTVTIESCLQHLYAAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPL
GSLMDGLMKLLIQHYTCLKSLTKHYISSYTSDTSIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKV
IMVIEDFNKHIILLSKKTKSKNRLTDYLHLGTMRDFDIKSTDLRAAIERSISDGRNISTEDSNAEDRDEDEDENENVEEDDDAQSTTYPEDLEEKEDECL
VIEKKEERRRRRAPSSEAEDPQPKRKRGRKAAEQLEAEDEEQVEEEQPETTLKLRRNKKSGAKNQKECGH*
AEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQI
DKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLSLDPCRRPMRSLGEKGRFDELELLIQNTPIEKLKETLKSTMTRSEGITYWNYLLRGFNPE
SKDALAKRFECVRSFMDGLSSTELSYKQTFDLITRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRD
TTVRSIATMQWPASILTPIADMFKTMNLSSGEIVTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLD
SIDLFSDKELREAEETILHHLNYCTMYKLTEKHLAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVW
YRDTLQRKQVDLQQVLTVLIDQNKDGKDVITPGLVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADT
YTVSECMKPIQSVLEHMQWLSGEQSMRMMNFILPLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGY
SIMTQNTLGSRSNPQRNFDMLTLEIIGMLRNCLQQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSS
DDQHMEIQLKDNLGKLIQFVANCLAVFDRAPSGYDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQI
LPLFNQHCKLLESLKALSDASKKAHKQLKQTAKNNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLS
VTASRVESIRLEPDYKQLAYSKRTFKQLTDITKVIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYT
IQDVLDDFMTEYGTDDSICKNEFVSKLPVYLLESLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHV
LGVQNEDAPSESVELTLKSISTVTIESCLQHLYAAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPL
GSLMDGLMKLLIQHYTCLKSLTKHYISSYTSDTSIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKV
IMVIEDFNKHIILLSKKTKSKNRLTDYLHLGTMRDFDIKSTDLRAAIERSISDGRNISTEDSNAEDRDEDEDENENVEEDDDAQSTTYPEDLEEKEDECL
VIEKKEERRRRRAPSSEAEDPQPKRKRGRKAAEQLEAEDEEQVEEEQPETTLKLRRNKKSGAKNQKECGH*
Vector Type
Baculovirus
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Various silent changes, plus residue differences:
A8G
P224T
K947M.
Extra residues at C-terminus: SGAKNQKECGH*
Recommend growth at 30 degrees.