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Plasmid
pSCB USP30 Cter GFP donor guide protected
Parent Plasmid
pSC-B
DU Number
DU48996
Genbank
NM_032663.3
Species
Human
Sequence of Insert
View Sequence
GGATCCGTCCAAAATGGAAATAACTTTTAAATTAAAAATTTTTAAAGTACATAAAATTTACATATGCATATGTATGTGTGTATATATGTGTGTATGTATAAAATATAAAGAAAATTTAAAATTTCTTGGGATACCATCTAGAGATAGCCAATTAAACACTTAGGGTCATGCACTCTATAAAGTTGTACATCACACATACATATAGTGTACATAAACACATACACTCATTGATACAAACATCCACATATACACAATATACATATACTCATAGAATGTACATCTTGCCATGCCAGTAAATACAGATCTGTATTTTAATAGTTGTGTAGCATTTGGTGGTATGGACTTACCATTGTATTCATCCCCTATTTAACATTAGCATTCTTTTGTTTTTGCCTATAAAGTCTTTTTGTTAAAATTGTAAACCCCTGACATGTGTTCGTATCATTCAGCTCCTCCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCTTACAAGAGGTCTTGAGTTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTAGTCGTATGCAGCATCAGTCTCAGGAGTGCAAGTCTGAAGAAGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGACTGTGCCCTCCTGCAATTCTAGAGCTGATGGCACTGTCTGCACTGTCCAGGAAAAAAGTAAAACTGTACTGTTGCGTGTGCAAGCGGCCCCACTAGAGCCTTCCAGCCTTCTGGTGTGTTCTAAGAGCAGGCTCCACCTGGGAGCCAGCCCCAGTTCACACCAAACCAGGCTCCCTGAACAGTCCTGTTCATGTGTGTAGGTGGTTCTGTTGTGTTAAGAAAGCATTCATTATGTCCGGAGTGTCTTTTTACTCATCTGATACAGGTAATTAAAAGAACTCAGATTCTTGAAGCCACCGTTTTCATATTGTAATGTTAGGTGTTCTCAGAGGGGAGGTACCTTTGTCTAATCAACGTTTCCACTTAGATCTTTTATTTTTAATAAGCAGGCCCATAAAAATTGTTGACAAGAATTAATGAAATTATTAAAGGCAACAATTTAGAAGAAAAAGTGCCTTTCACTTTCGATTGCTTTTGTAGCACGTCCATTGTGAAATATTCCTTCCAGGCTACTCAAAGGATAGCAAGAGAACAGGTAAATGATGCCTAAAGAACACCGCGGCCGC
Vector Type
Other
Antibiotic
Amp
Comments
CRISPR donor plasmid. Designed to KI a GFP reporter to the c-terminus of USP30 when co-transfected with guide pairs 48939/48953 or 48939/48954. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary. Modified to prevent targeting by guides.
100042 R 1000 1001 1047 19007
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