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Plasmid
pBABED P U6 USP24 KO sense
Parent Plasmid
pBABED P U6
Expressed
USP24 KO sense
DU Number
DU48890
Genbank
NM_015306.2
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
Comments
CRISPR guide plasmid. Targets sense strand in exon 10, designed to KO USP24 when co-transfected with antisense guide 52013. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty flp-in line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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