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Plasmid
pSc-B-(GGSG X2) SENP1 (aa415-602)-(GGSG)-eGFP-SV40-SENP1 C603A (aa415-644)
Parent Plasmid
pSC-B
DU Number
DU24760
Species
Human
Sequence of Insert
View Sequence
AAGCTTGATAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTCAGCAGATGAATGGAAGTGACGCTGGGATGTTTGCCTGCAAATATGCTGACTGTATTACCAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATACTTCCGGAAGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTAGGTCGAC
Vector Type
Other
Antibiotic
Amp; Kan
Comments
cloned overlap PCR product - SENP1 fragment with 5' and 3' linkers fused to eGFP-SV40 sequence. C603A SENP1 fragment then inserted into HindIII-Sal1 sites. 33bp of pSc-B sequence exists between nt1570-1601 (NW3414/3415 to remove). Entire SENP-eGFP-SV40-SENP C603A cassette can be excised with BamHI-Sal1.
100042 R 1000 1001 1047 19007
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