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Plasmid
pETDuet-1 GST Thrombin SAE1, 6-His Precission SAE 2 C173A
Parent Plasmid
pETDuet1
Expressed
6His-SAE2 C173A
DU Number
DU24687
Genbank
NM_005499.2
Tag
6His
Species
Human
Sequence of Insert
View Sequence
CCATGGGACACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCA
ATGGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGG
GGGCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCA
AGAATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGAT
ACTATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACA
TGTTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTT
ACCCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGAC
TATAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGA
TAACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATG
TTCCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACT
ATCAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCA
GAGAACCTTTCCTGGCgcTACAATTCGTAACACACCTTCAGAACCTATAC
ATTGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAA
GATGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTG
GGAACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTG
ACATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGAT
CCAGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTT
GACAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACT
GGGCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAG
AATGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAG
CTATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAG
CAGAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCT
GCAATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAG
TATGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTA
TTCCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTG
GAAGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTT
TTTGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCAC
TGGATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTG
ACTGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAA
GATAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAG
ATGGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCT
AATAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCT
TCAAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTC
ATAGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCC
CCGGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAAC
CAATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGC
AAGATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAAT
GCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAA
AGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTG
ATGATGTCATAGCATTAGATTGAGTCGAC
ATGGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGG
GGGCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCA
AGAATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGAT
ACTATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACA
TGTTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTT
ACCCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGAC
TATAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGA
TAACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATG
TTCCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACT
ATCAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCA
GAGAACCTTTCCTGGCgcTACAATTCGTAACACACCTTCAGAACCTATAC
ATTGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAA
GATGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTG
GGAACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTG
ACATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGAT
CCAGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTT
GACAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACT
GGGCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAG
AATGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAG
CTATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAG
CAGAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCT
GCAATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAG
TATGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTA
TTCCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTG
GAAGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTT
TTTGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCAC
TGGATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTG
ACTGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAA
GATAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAG
ATGGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCT
AATAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCT
TCAAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTC
ATAGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCC
CCGGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAAC
CAATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGC
AAGATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAAT
GCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAA
AGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTG
ATGATGTCATAGCATTAGATTGAGTCGAC
Amino Acid Sequence
View Sequence
MGHHHHHHLEVLFQGPMALSRGLPRELAEAVAGGRVLVVGAGGIGCELLK
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGATIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD*
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGATIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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