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Plasmid
pET15b 6His 3C- DCUN1D1-Ub (KallR)
Parent Plasmid
pET15b 6His 3C
Expressed
6His-DCUN1D1-Ubiquitin (KallR)
DU Number
DU24684
Genbank
NM_020640
Tag
6His
Species
Human
Sequence of Insert
View Sequence
gcggccgctggcggctccggcggaggttccggaATGCAGATATTCGTGAG
GACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGAGTGACACCA
TTGAGAATGTCAGGGCAAGGATCCAAGACAGGGAAGGCATCCCTCCTGAC
CAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACGCACCCT
GTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCCTCCGTC
TCAGAgctgcgTGAgcggccgc
GACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGAGTGACACCA
TTGAGAATGTCAGGGCAAGGATCCAAGACAGGGAAGGCATCCCTCCTGAC
CAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACGCACCCT
GTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCCTCCGTC
TCAGAgctgcgTGAgcggccgc
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLEVLFQGPGSMNKLKSSQKDKVRQFMIFTQSSEKTAV
SCLSQNDWKLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQD
ENKIGIDGIQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMT
ELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEM
AIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDM
SNYDEEGAWPVLIDDFVEFARPQIAGTKSTTVAAAGGSGGGSGMQIFVRT
LTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLS
DYNIQRESTLHLVLRLRAA*
SCLSQNDWKLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQD
ENKIGIDGIQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMT
ELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEM
AIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDM
SNYDEEGAWPVLIDDFVEFARPQIAGTKSTTVAAAGGSGGGSGMQIFVRT
LTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLS
DYNIQRESTLHLVLRLRAA*
Vector Type
Bacterial
Antibiotic
Amp
Comments
DCUN1D1 carries g318a mutation (silent Ala106). vector 24673
100042 R 1000 1001 1047 19007
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